In order to cultivate students' quality of scientific research thinking,self-learning ability,innovation ability and practical ability,Open Innovation Experiment Project which is suitable for the undergraduate students has been carried out in Jilin university.The feature of experiment is based on the teacher's scientific research project,and the experimental design is completed by the students independently.By the stage inspection as a monitoring mechanism,the overall effect evaluation of the experimental project has been conducted by the combination of the process and the results.Through the selection,design,implementation and results of practice,students can not only cultivate innovative consciousness and comprehensive thinking and the spirit of unity and cooperation,but also have a basic understanding of scientific research work.

Ninety-two cases of enuresis were treated with auricular point pressure onthe kidney (MA-SC), bladder (MA-SC 8), lung (MA-IC 1), heart (MA-IC), liver (MA-SC 5), Er Shenmen(MA-TF 1),Zhen (MA-AT) and Subcortex (MA-AT 1) plus tuina. After treatment, 61 cases were cured, 28 cases were effective and 3 cases were ineffective, with an effective rate of 96.7%.

To observe the therapeutic effect of acupuncture and moxibustion on the climacteric syndrome, patients with climacteric syndrome were treated by acupuncture and moxibustion and randomly compared with the patients in control group treated by Chinese herbs. There was a significant difference between two groups (P＜0.05). The results showed that the therapeutic effect in acupuncture and moxibustion group was obviously better than that in Chinese herbs group.

Hearing Loss ; complications ; diagnosis ; metabolism ; Humans ; Multiple Acyl Coenzyme A Dehydrogenase Deficiency ; complications ; diagnosis

Hearing Loss, Central ; genetics ; Humans ; Vestibulocochlear Nerve Diseases

Purpose To study the clinicopathologic features, diagnosis and differential diagnosis of extranodal NK/T-cell lymphoma, nasal type ( EN-NK/TCL) . Methods Twenty-eight cases of EN-NK/TCL were analyzed according to WHO classification of tumors of haematopoietic and lymphoid tissues, the cases were studied by microscopy, immunohistochemistry and in situ hybridization. Results In 28 cases, the male to female ratio was 1 ∶ 1. 2. The mean age was 46 years, with the median of 44. 5. Eighteen cases involved nasal cavity, 5 cases of skin, and 1 case of tonsil, upper palate, root of tongue, adrenal glands and gastric, respectively. The main presentation was nasal obstruction in cases with nasal involvement, and the cases with skin involvement present with rash, ulcer, plaque or nodular mass. Patients might be accompanied by B-type symptoms, lymphoadenopathy or with multiple sites involvement. The tumor cells were small, medium, large-sized, or mixed, and angiocentric and angiodestructive growth pattern was frequently pres-ent. Most cases were mixed with inflammatory cells infiltration. The typical immunophenotype was T-cell ( the most sensitive, CD3ε) , CD56 and cytotoxic markers. In situ hybridization for EBER was positive. Ten cases were followed up, and 1 case died. Conclusions The most common site of EN-NK/TCL is nasal cavity, followed by skin. EN-NK/TCL can be definitely diagnosed by integration of pathological morphology, immunophenotype and in situ hybridization of EBER.

The authors described two cases with primitive trigeminal artery. Case 1 was a 32-year-old woman who suffered dizziness and a serious pulsatile intracranial bruit on the left ear, and sometimes associated with pulsatile intracranial bearing-pain on the left temporal side six months before she was admitted to the hospital. She also suffered from obvious diplopia on left lateral gaze for the last 5 months. She had suffered no recent trauma. Magnetic resonance imaging(MRI) demonstrated a suspected intracranial aneurysm located in left cavernous sinus. Digital subtraction angiography (DSA) was performed and a primitive trigeminal artery-cavernous sinus fistula in left side was found. Intraluminal occlusion of the fistula was successfully performed immediately after angiography using 6 Guglielmi detachable coils (GDC), and the patient was cured finally. Case 2 was a 28-year-old woman who suffered a serious intermittent cephalodynia associated with soreness on the left body two years before she was admitted to the hospital. She had suffered no recent trauma. Magnetic resonance angiography(MRA) demonstrated a suspected intracavernous aneurysm of the right internal carotid artery, Digital subtraction angiography (DSA) was performed. Right internal carotid angiography showed a primitive trigeminal artery (PTA) run between the cavernous segment of the internal carotid artery and the distal portion of the basilar artery. On initiation of PTA of R-ICA a small wide-necked saccular aneurysm was incidentally visualized. The aneurysm was successfully embolized after angiography using 2 Stent (Neuroform, 4.5mmm × 20mmm)-assisted detachable coils (Matrix), the ICA and PTA were preserved, and the patient was cured finally.

Objective :To sutdy the effects of Shenyanling on chronic glomerulonephritis in rabbits. Meth-ods:Rat glomerular base membrane (GBM) and Freund＇s adjuvant were injected into rabbits subcuta-neously or intracutaneously to build the Steblay nephritis model. Shenyanglin was administered. Kidneyfunction was evaluated by measuring urine protein,urea nitrogen ,creatinine ,albumin and globulin in serumas wellas the histopathological changes of kidney were observed. Results:Compared with model group,Shenyanling could dose-dependently reduce the level of the urine protein,urea nitrogen and creatinine inserum and the damage of kidney;while,the serum albumin could maintain the normal level (P＜0.05).Conclusion:Shenyanling has protective effects on chronic glomerulonephritis in rabbits.

Objective To develop a balloon-type head fixation pillow for interventional neuroradiology operation.Methods Some radiotransparent sponge with high elasticity and strength and some nonopaque cloth with high skin compatibility were employed to make pillow base adaptable to the posterior fossa, neck and shoulder. A inflatable balloon made of anti-tensile airtight nylon was used to fix the underjaw.Results The fixation pillow gained advantages over the common one in imaging times, X-ray exposure dose and abnormal condition.Conclusion The head fixation pillow may decrease operating time, exposure dose and complications during interventional neuroradiology operation, and thus is worth popularizing clinically.

Objective To establish a multiplex real-time PCR mothed for rapid detection of seven species of intracranial bacteria after surgery. Methods Firstly ,the Gram′s identification was deter mined. Secondly , according to the results of Gram identification ,the bacterium was typed by the specific primers and probes to deter mine the distribution of bacteria. Simul taneously ,the sensitivity and specificity of this method were verified by making intracranial infected sim ulated samples and contrasting national standard method. Results The method established could complete detention within 4 hours with a good specificity. Staphylococcus aureus,Streptococcus pneumoniae ,Escherichia coli ,Pseudomonas aeruginosa pseudomonas ,Klebsiella pneumoniae and Acinetobacter baumannii could be detected at concentrations of ≥102 CFU/mL. Enterococcus faecalis could be detected at concen-trations of≥103 CFU/mL. The lowest detection limita of this method is higher than culture method for Streptococcus pneumoniae. Conclusions Real-time Multiplex PCR method was with high sensitivity and specificity. It reduced the detection time greatly and has great value in early diagnosis of bacteria in intracranial infection. It should be of great significance for guiding clinical treatment.