Objective To clarify the genotype of wild-type strains of varicella zoster virus (VZV) in Jilin province in 2014,and to discriminate between v-Oka vaccine strains and wild-type strains.Methods Vesicle fluid and throat swab samples were collected from 13 individuals with suspected VZV in Jilin province from January to December 2014.Viral DNA was extracted,the fragments of 15 open reading fragments (ORFs) were amplified by polymerase chain reaction (PCR),and viral genotypes were determined by single nucleotide polymorphisms (SNP).PCR restriction fragment length polymorphism (RFLP) was used to distinguish between wild-type strains and v-Oka vaccine strains.The results were analyzed with MEGA5 software,using the VZV reference strain sequences from GenBank.Results The 13 suspected samples included 5 males and 8 females,aged 11-27 years (mean:(16.69±5.48) years).Sampling was performed on days 0 to 3 of suspected infection.VZV strains were detected in 8 samples,all belonging to Clade 2.There was a synonymous mutation (T＞C) in SNP18082 compared with the v-Oka vaccine strain.Analysis of PCR-RFLPs showed that all 8 positive samples were wild-type strains (Pst Ⅰ +Bgl Ⅰ +Sma Ⅰ-).Conclusions The study revealed that the VZV strains circulating in Jilin province in 2014 were wild-type strains belonging to Clade 2.

Objective To clarify the genotype of wild-type strains of varicella zoster virus (VZV) in Jilin province in 2014,and to discriminate between v-Oka vaccine strains and wild-type strains.Methods Vesicle fluid and throat swab samples were collected from 13 individuals with suspected VZV in Jilin province from January to December 2014.Viral DNA was extracted,the fragments of 15 open reading fragments (ORFs) were amplified by polymerase chain reaction (PCR),and viral genotypes were determined by single nucleotide polymorphisms (SNP).PCR restriction fragment length polymorphism (RFLP) was used to distinguish between wild-type strains and v-Oka vaccine strains.The results were analyzed with MEGA5 software,using the VZV reference strain sequences from GenBank.Results The 13 suspected samples included 5 males and 8 females,aged 11-27 years (mean:(16.69±5.48) years).Sampling was performed on days 0 to 3 of suspected infection.VZV strains were detected in 8 samples,all belonging to Clade 2.There was a synonymous mutation (T＞C) in SNP18082 compared with the v-Oka vaccine strain.Analysis of PCR-RFLPs showed that all 8 positive samples were wild-type strains (Pst Ⅰ +Bgl Ⅰ +Sma Ⅰ-).Conclusions The study revealed that the VZV strains circulating in Jilin province in 2014 were wild-type strains belonging to Clade 2.

Objective To explore the effects of focused settlement mode applied in caring the acute paraquat poisoning patients.Methods 106 hospitalized patients with acute paraquat poisoning were randomly divided into the control group and the experimental group.All patients in the control group were performed conventional nursing instruction and the experimental group was nursed with focused settlement mode on the basis of conventional nursing instruction.The efficacy of nursing was evaluated when they were discharged from hospital.Results The self-care skills and knowledge,consciously following the medical behavior,satisfaction degree with clinical service of the experimental group were significandy better than the control group,while the hospitalization days were shorter than the control group.Conclusions Focused settlement mode can improve the effect of nursing care for patients with acute paraquat poisoning.