Objective To study the effect of hypoxial endothelia cell conditional medium(HECCM)on the proliferation of pulmonary arterial muscle cell(PASMC).Methods MTT assay was used to test the proliferation,immuno-cytochemistry was used to identify the expression of ?-SM-actin.Results(1)HECCM could promote the proliferation of PASMC,down-regulate their expression of ?-SM-actin.(2)DDPH could up-regulate the expression of ?-SM-actin in PASMC which was time-dependant.Conclusions DDPH could reverse the phenotype transformation of PASMC exposed to HECCM,the action was time-dependant.After some time DDPH could reversly transform PASMC to the normal contractile phenotype.

Objective:To observe expression level change of miRNA?126 in the myocardial tissue of rats with myocardial infarction and to observe the influence of Shuxuetong injection. Methods:Myocardial infarction model was established in 80 SD rats, 60 surviv?al rats were randomly divided into Shuxuetong group and model group, the other 30 rats were randomly selected as control group. After the success of the modeling, Shuxuetong group received Shuxuetong injection treatment, model group and control group received same amount of normal saline. Real?time quantitative PCR was used to detect miRNA?126 expression levels in rat myocardial tissue at dife?rent time after operation. Results: After 7 days and 14 days, miRNA?126 expression levels in rats myocardial tissue in Shuxuetong group and model group were significantly lower than that in the control group ( P<0?05) , but there was no difference between the two groups. After 14 days, miRNA?126 expression levels in the Shuxuetong group were higher than that after 7 days ( P<0?05) . Conclu?sions:miRNA?126 in 7 days after myocardial infarction significantly reduced. With the passing of time, miRNA?126 expression levels increased gradually and Shuxuetong can increase the expression levels of miRNA?126 .

Objective To observe the ehanges in plasma OX40L level in patients with stable angina pectoris (SAP) after percutaneous coronary intervention (PCI) and to investigate the relationship between OX40L level and prognosis in patients with SAP.Methods We collected 200 cases of SAP after PCI.Plasma OX40L levels were examined at different time points,including before and after stent placement,in all patients withSAP.The patients with SAP were followed up for 18 months,and the end point was adverse cardiovascular events.Results The plasma OX40L level increased more apparently after PCI than before PCI (P ＜ 0.05) and peaked at 24 hours.We followed up 185 patients successfully,including 39 (21.08％) patients with and 146 (78.92％) without adverse cardiovascular events.The mean maximum OX40L level was higher in patients with than in those without adverse events (P ＜ 0.05).Multivariable logistic regression analysis revealed that the mean maximum OX40L level was related to adverse cardiovascular events (P ＜ 0.05).Conclusion PCI may cause upregulation of plasma OX40L expression.Mean maximum OX40L level showed good clinical predictive value of occurrence of cardiovascular events 18 months after PCI.

Objective:To investigate the effect of helicobacter pylori (Hp) infection and gastric polyps on colorectal cancer lesions.Methods:The clinical data of 2 034 patients with colorectal polyps and 118 patients with colorectal cancer detected by gastroscopy and colonoscopy examination in General Hospital of Beijing Jingmei Group from January 2020 to June 2021 were retrospectively analyzed. A total of 178 patients without colorectal polyps in the same period were treated as controls to analyze Hp infection and gastric polyps in patients with different ages, genders and pathological types.Results:The age and the proportion of male patients in colorectal cancer group were higher than those in the control group and colorectal polyps group (all P < 0.05). The Hp infection rates of the control group, colorectal polyps group and colorectal cancer group were 20.8% (37/178), 23.0% (467/2 034) and 27.1% (32/118), respectively, and the differences were statistically significant (all P > 0.05). Among 2 034 cases of colorectal polyps, there were 612 cases of inflammatory polyps, 371 cases of proliferative polyps and 1 051 cases of adenomatous polyps. The infection rates of Hp in the three kinds of colorectal polyps were 24.5% (150/612), 22.4% (83/371) and 22.3% (234/1 051), respectively. The incidence of patients with gastric polyps in the 3 groups was 34.6% (212/612), 38.3% (142/371) and 39.3% (413/1 051), respectively, and the differences were statistically significant (all P > 0.05). The age of the three colorectal polyps groups was significantly different from that of the control group and colorectal cancer group (all P < 0.05) except for inflammatory polyps and proliferative polyps groups. The proportion of female in three kinds of colorectal polyps combined with gastric polyps was higher than that in male patients (all P < 0.05). The age of Hp positive patients in the control group, different types of colorectal polyps groups and colorectal cancer group was lower than that in negative patients, but there was a statistically significant difference only in adenomatous polyps group ( P = 0.002). The age of patients with different types of colorectal polyps combined with gastric polyps was older than that of those without gastric polyps, and the age of patients with colorectal cancer combined with gastric polyps was younger than that of those without gastric polyps (all P < 0.05). There was no assocaiton of Hp infection and gastric polyps with colorectal polyps and colorectal cancer (all P > 0.05). Conclusions:Hp infection may promote the early occurrence of colorectal adenomatous polyps. There is no evidence that gastric polyps are associated with the risk of colorectal polyps. Female patients with colorectal polyps have a higher risk of gastric polyps.