[Objective]The relationship of inheritance and innovation is one of most important problems of TCM.Wind of historyis a historical theory pro-posed by Liu Xianxin. We use the theory to analyze the inheritance and innovation in the history of TCM, so that we can identify the relationship of inheri-tance and innovation of TCM under current background. [Methods]Based onWind of historytheory, we try to analyze classic innovated case in the history of TCM.After that, we analyze the current relationship and controversy of inheritance and innovation of TCM. [Results]We find that al innovation comes from the interaction of inheritance and the Wind of history. Different innovation comes from the interaction of different problems and different wind. [Conclusions]We need not make innovation as the purpose or requirements imposed on TCM. If we have better inheritance, innovation is just there when we confront new problem.

Objective To analyze the characteristics of differential diagnosis in medical record of ZuiHuaChuang, so that to improve level of medical diagnosis and treatment in clinical work. [Methods] Analyze and research 58 cases of medical records which involved in differential diagnosis in the book, to summarize the characteristic of differential diagnosis inmedical record of ZuiHuaChuang. [Results] There are 58 cases in 101 cases medical records in the whole book written by WangYu in Qing Dynasty. The differential diagnosis methods base on perimal symptoms, concomitant symptoms and pulse diagnosis, and the pulse diagnosis is attributed to the most importantly. The differential diagnosis based on pulse diagnosis is the key charcteristic ofmedical record of ZuiHuaChuang.

Objective] To analyze the character of diagnosis and treatment of excessive fluid zheng in medical record of ZuiHuaChuang, so that to improve level of medical diagnosis and treatment in clinical work. [Methods] Analyze the diagnosis,treatment and nurse of medical records of excessive fluid zheng in the book, to summarize the characteristic of diagnosis and treatment of excessive fluid zheng in medical record of ZuiHuaChuang. [Results] There are 6 cases involvingexcessive fluid zhengin 101 cases medical records in the whole book which written by WangYu in Qing Dynasty. The key symptoms of excessive fluid zheng in medical record of ZuiHuaChuang including difficulty in micturition,dyspnea,thirsty,edema, cardiopalmus, vomiting, insomnia and string-tight pulse accompanied with rapid or stasis pulse. It was treated with WuLin San most of time, and used the method ofinvigorating spleen to eliminate dampnessto regulate the constitution.[Conclusion] The character of diagnosis and treatment ofexcessive fluid zheng in medical record of ZuiHuaChuang including:1. diagnosed by pulse diagnosis, especial y combined with symptoms,2.Treat with WulinSan,and emphasize regulation of constitution after erasing excessive fluid.

[Objective] Analyze the discussion of Qi and Qi transformation in Huangdi Neijing, therefore to give insight into the Qi Theory of TCM. [Methods] Analyze articles about Qi and Qi transformation in Huangdi Neijing, as well as comments from doctors of all dynasties, to summerize the understanding on Qi and Qi transformation of Huangdi Neijin. [Result] In the whole book of Huangdi Neijing, there are 19 chapters named after Qi, 1700 (2997) characters of Qi are mentioned, and there are 7 discussions upon Qi transformation. The Qi mentioned in Huangdi Neijing can be material, or functional, or be able to transform. The conception of Qi transformation includes six parts: the transformation among Yin and Yang, among the five elements, and the growth and transformation among all kinds of essences, the transportation, nutrition and transformation among all kinds of matters, as well as the transportation of Qi and the transformation of five elements' motion and six kinds of natural factors.[Conclusion] Qi transformation is mentioned in all but a few chapters in Huangdi Neijing. Everything, from matters growing to turning into essences is actually Qi transformation. In Huangdi Neijing's view, Qi transformation is the fundamental of all matters coming into being, and the fundamental to stay healthy, therefore the fundamental of preserving health, diagnosis and treatment.

Objective] The article analyzed the application of qi transformation for diagnosis and treatment in clinic from four aspects: theory, method, formula and herbs. [Methods] Wang Kungen is instructor of the fourth,fifth project of academic experience inheriting the old Chinese medicine experts. Director Wang believes that the Qi transformation is the fundamental of everythings,including the humanbody.To analyze the relationship between qi transformation and theory-method-formula-herbs in clinical TCM based on the study of Huangdi Neijing and textual works of famous Chinese physicians. [Result]Qi transformation is the foundation of life, therefore the foundation of health. Abnormal Qi transformation leads to process and development of diseases, so the key to the treatment is to achieve the condition of“seizing the mechanism of disease and keeping qi in harmony”through all methods, rather than holding on to specific treatments like acupuncture and herbs. [Conclusion]Qi transformation is not only an important content of TCM theory system, but also the key point of treatment in clinic, and the core clinical theory of Director Wang.

[Objective] A randomized control ed trial of traditional Chinese medicine recipe Gujing Tang treatment of premature ejaculation was studied. [Methods] The subjects: 60 patients with premature ejaculation form department of andrologic outpatient, the patients were randomly divided into two groups, Integrated TCM-WM Groups and paroxetine Groups. 30 cases in each group. The Integrated TCM-WM Groups were given Gujing Tang , with paroxetine 10mg, oral, Qd at the same time; The paroxetine groups were given paroxetine 10mg, oral, Qd. Both groups were treated for 3 months. Ejaculation status was evaluated at the timepoint:before treatment, 3 months after treatment,and fol ow-up evaluation of six months after treatment. Evalu-ate parameter including Intravaginal ejaculation latency(IELT) and score of sexual function and Chinese patients with premature ejaculation evaluation -5 (CIPE-5). [Results] After 3 months, both groups were significantly improved, and Integrated TCM-WM Groups improved more significantly. After 6 months of fol ow-up, IELT, motion times and CIPE-5 score of Integrated TCM-WM Groups decreased slightly, but stil had significant difference com-pared to before treatment, and this parameter of Paroxetine groups was returned to the level of baseline, and had no significant difference compared with baseline. [Conclusion] Traditional Chinese medicine Recipe Gujing Tang combined with paroxetine, could not only have rapid onset, enhance patient confidence, improve the psychological state, but also improve the long-term efficacy and avoid recurrent disease after treatment, therefore can achieve ef-fective treatment of premature ejaculation.

The article introduces the training of basic skill,renovating of the experiment contents,the diversifi cation and abundance in interest of experiment teaching methods of the students of medical laboratory speciality of higher profession.

ObjectiveTo investigate the applicability and superiority of modified Seldinger technique (MST).MethodsTotally 108 patients receiving peripherally inserted central catheter (PICC) through vascular ultrasound combined with MST technology were enrolled as MST group and another 108 patients receiving PICC using traditional direct vision method as control group.The first puncture success rate,PICC succesa rate,catheterization time required,and early complication rate were compared between these two groups.ResultsThe first puncture success rate was 88.89％ in MST group and 72.22％ in control group (P=0.002) ; the catheterization success rate was 82.41％ in MST group and 57.41％ in control group ( P =0.000).In the MST group,there were two cases where the catheter within neck was ectopic ( P =0.003 ),two cases experienced bleeding at the puncture sites (P =0.017),three cases had phlebitis (P =0.001 ),and one case suffered from catheter blockage (P=0.010).In the eontrol group,the corresponding numbers were 13,10,18,and 9.However,the time required for PICC showed no significant difference between these two groups (P=0.685).In the MST group,15 cases were catheterized in less than 15 minutes,72 cases between 15 and 25 minutes,and 21 cases for more than 25 minutes.In the control group,12 cases were catheterized in less than 15 minutes,70 cases between 15 and 25 minutes,and 26 cases for more than 25 minutes.ConclusionPICC through MST can be more effective in positioning the targeting blood vessels,increasing the success rate of catheter insertion,reducing various tissue damages,and lowering potential complications.

Objective To investigate the effects of fluid shear stress on interleukin-8(IL-8)mRNA expression in human endothelial cell line-EA.Hy926 cells.Methods Weibel-Palade body and factor Ⅷ related antigen were detected to identify cultured EA.Hy926 cells.Quantitative reversal transcription-polymerase chain reaction was also used to assay IL-8 mRNA expression.Results It was found that the growth feature of EA.Hy926 cells in vitro culture was similar with that of human umbilical vein endothelial cells(HUVECs).Meanwhile,it also had the typical features of endothelial cells,i.e.Weibel-Palade body in plasma and express factor Ⅷ related antigen.IL-8 mRNA expression of endothelial cells exposed to low shear stress(0.420 Pa)increased at 1 h and reached its peak value at 2 h,then gradually decreased at 3 h and kept the descending trend throughout the remained time course of the study,as compared with that in cells not treated with shear stress.Also after exposed to shear stress of different levels(0.182,0.420,1.000,1.640 Pa)for 2 h,in which IL-8 mRNA expression of EA.Hy926 cells decreased with the increase of the intensity of the shear stress.Conclusion The results suggest that fluid stress can induce the expression of IL-8 mRNA in EA.Hy926 cells.EA.Hy926 cells might be used as a cell source in the field of biorheological research of endothelial cells.

The paper quantizes symptom data through binary coding,divides 8 syndromes summed up by experts into excess and de-ficiency syndromes,values and quantizes them,and establishes the model for classification of excess and deficiency syndromes of colorec-tal cancer based on BP neural network and decision tree.The result shows that BP neural network classification model is more applicablefor the handling of the nonlinear mapping relation compared with decision tree classification model.