1.Total serum bilirubin and bilirubin to albumin values in predicting neonatal acute bilirubin encephalopathy: a multicenter study
Xiaofan SUN ; Qiufen WEI ; Zhankui LI ; Jie GU ; Jing QIAN ; Danhua MENG ; Jinzhen GUO ; Xiaoli HE ; Hui NAN ; Zhangbin YU ; Shuping HAN ; Xiaoyue DONG
Chinese Journal of Neonatology 2022;37(1):25-29
Objective:To study the predictive value of total serum bilirubin (TSB) and the ratio of bilirubin to albumin (B/A) in neonatal acute bilirubin encephalopathy (ABE).Methods:Neonates with extremely severe hyperbilirubinemia (TSB≥425 μmol/L) treated in the Nanjing Maternal and Child Health Hospital, Maternity and Child Health Care of Guangxi Zhuang Autonomous Region, Northwest Women and Children's Hospital, Yinchuan Maternal and Child Health Hospital and Liaocheng People's Hospital from March 2018 to August 2019 were selected as prospective subjects for this study. According to the score of brain injury induced by bilirubin, the subjects were divided into ABE group and non-ABE group, and the predictive value of TSB peak and B/A for neonatal ABE were analyzed.Results:A total of 194 infants with extremely severe hyperbilirubinemia were recruited in this study, including 20 in ABE group and 174 in non-ABE group. The peak value of bilirubin ranged from 427 to 979 μmol/L. The optimal critical values of TSB peak value and B/A for ABE prediction were 530 μmol/L and 9.48, respectively. The sensitivity and specificity of ABE prediction were 85.0% and 92.8% when combined with TSB peak and B/A values.Conclusions:TSB peak combined with B/A value can effectively identify neonatal ABE. When the TSB peak value was greater than 530 μmol/L and the B/A value was greater than 9.48, the neonates had a higher risk of neonatal ABE.
2.Targeting TRMT5 suppresses hepatocellular carcinoma progression via inhibiting the HIF-1α pathways.
Qiong ZHAO ; Luwen ZHANG ; Qiufen HE ; Hui CHANG ; Zhiqiang WANG ; Hongcui CAO ; Ying ZHOU ; Ruolang PAN ; Ye CHEN
Journal of Zhejiang University. Science. B 2023;24(1):50-63
Accumulating evidence has confirmed the links between transfer RNA (tRNA) modifications and tumor progression. The present study is the first to explore the role of tRNA methyltransferase 5 (TRMT5), which catalyzes the m1G37 modification of mitochondrial tRNAs in hepatocellular carcinoma (HCC) progression. Here, based on bioinformatics and clinical analyses, we identified that TRMT5 expression was upregulated in HCC, which correlated with poor prognosis. Silencing TRMT5 attenuated HCC proliferation and metastasis both in vivo and in vitro, which may be partially explained by declined extracellular acidification rate (ECAR) and oxygen consumption rate (OCR). Mechanistically, we discovered that knockdown of TRMT5 inactivated the hypoxia-inducible factor-1 (HIF-1) signaling pathway by preventing HIF-1α stability through the enhancement of cellular oxygen content. Moreover, our data indicated that inhibition of TRMT5 sensitized HCC to doxorubicin by adjusting HIF-1α. In conclusion, our study revealed that targeting TRMT5 could inhibit HCC progression and increase the susceptibility of tumor cells to chemotherapy drugs. Thus, TRMT5 might be a carcinogenesis candidate gene that could serve as a potential target for HCC therapy.
Humans
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Carcinoma, Hepatocellular/pathology*
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Cell Hypoxia
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Cell Line, Tumor
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Gene Expression Regulation, Neoplastic
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Hypoxia-Inducible Factor 1, alpha Subunit/metabolism*
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Liver Neoplasms/pathology*
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Signal Transduction/genetics*
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tRNA Methyltransferases/metabolism*