After purifying thyroglobulin (TG) and thyromicrosomal (TM) antigens,the ELISA (enzyme-linked immunosorbent assay) for detecting anti-TG,anti-TM,serum TG level,TG-antithyroglobulin immune complex (TG-IC) and IgG-,IgM-,IgA-specfic antithyroglobulin,antithyromicrosome were established.These specific immunplogical assays were sensitive,reproducible and did not have the shortcomings associated with the impurity of the TM antigen.The results of clinical studies in patients with Hashimoto's thyroiditis (n=35),hyperthyroidism (n=86),primary hypothyroidism (n=7) suggested that these specific assays were helpful to improve the diagnosis and investigation into pathogenesis for aformetioned autoimmune thyroid diseases (AITD).

The endometrial antigens from human endometrium were purified by collagenase (Type I), 250um, 40um, sieves and affinity chromatography. Indirect ELISA was used to detect antiendometrial antibodies (EMAb). The findings showed that the positive rates of EMAb in this assay were 43.4% (infertile, n=129), 37.1% (abortion, n=113), 6.7% (middle and old man, n = 120), 3.8% (pregnant woman, n = 52) and 0% (male, n = 43), respectively.

Abstract Enzyme linked immunosorbent assay (ELISA) was used to detect free endometrial antigens (EMAg) and their immune complex (EMAg-ICs) in the serum of pregnancy, endometri-osis (EMT). abortive and infertile women. EMAg levels were 16.4 ?11.5 (U/ml, xis) in pregnant women (n = 63), 56.0131.7, 53.9?44.6 and 44.3?26.0 in EMT (n = 30), abortive (n = 37) and infertile (n = 48) patients, respectively. EMT was considered as positive when the level was 40 U/ml or higher. The positive rates of EMAg in three patient groups were 56.7%, 32.4% and 31.3%. respectively; they were all significantly higher than pregnant women group (0, P