AIM:The paper is to explore a rapid and simple method for the culture of mouse primary thyroid cells.METHODS:Mouse thyroid cells were isolated by enzyme digestion and cultured with improved medium,and their morphology,characteristics and secretory function were observed within 14 d.RESULTS:In the cultures,the active pri-mary cells were obtained from the thyroid tissue after digestion for 25 min;adherent growth was observed on the 2nd day.And secondary follicles appeared from the 5th to 7th day.Over 95%cells were detected with thyroglobulin.The secretion of total triiodothyronine and total thyroxine maintains over 60%in 7 d.The expression levels of specific genes can still maintain more than 50%in 10 d.CONCLUSION:Mouse thyroid primary cells can be rapidly cultured by this method,and the cells can be used for studying thyroid endocrine secretion within 7 d and studying thyroid genes within 10 d.

Objective To investigate the impact of Coronavirus Disease 2019(COVID-19)on long-term renal function in patients with chronic kidney disease(CKD)who received no renal re-placement therapy.Methods Thirty-nine CKD patients with an estimated glomerular filtration rate(eGFR)＜60 mL/(min·1.73 m2)at the time of COVID-19 diagnosis,admitted to Guizhou Aero-space Hospital from December 2022 to March 2023,were enrolled in the COVID-19 group.Addition-ally,40 CKD patients without COVID-19 from September to December 2021,were included in control group.Changes in eGFR were compared between the two groups,and the temporal trend of eGFR in the COVID-19 group was analyzed using a mixed-effects linear model.Results No statistically sig-nificant differences were observed in eGFR levels at baseline,1-and 3-month of follow-up between the COVID-19 and control groups(P＞0.05).However,at 1 year of follow-up,the eGFR level in the COVID-19 group was significantly lower than that in the control group(P＜0.05).The mixed-effects linear model analysis revealed a decrease in eGFR by 1.84 mL/(min·1.73 m2)within 1 year of COVID-19 onset in the COVID-19 group,which inclined 4.62％from baseline,with the most significant decline(7.45％)observed among diabetic patients.Conclusion Patients with CKD who have not undergone renal replacement therapy experience a substantial decline in eGFR and worsened renal function within 1 year of COVID-19,necessitating close long-term renal function mo-nitoring and timely interventions.

Objective To investigate the impact of Coronavirus Disease 2019(COVID-19)on long-term renal function in patients with chronic kidney disease(CKD)who received no renal re-placement therapy.Methods Thirty-nine CKD patients with an estimated glomerular filtration rate(eGFR)＜60 mL/(min·1.73 m2)at the time of COVID-19 diagnosis,admitted to Guizhou Aero-space Hospital from December 2022 to March 2023,were enrolled in the COVID-19 group.Addition-ally,40 CKD patients without COVID-19 from September to December 2021,were included in control group.Changes in eGFR were compared between the two groups,and the temporal trend of eGFR in the COVID-19 group was analyzed using a mixed-effects linear model.Results No statistically sig-nificant differences were observed in eGFR levels at baseline,1-and 3-month of follow-up between the COVID-19 and control groups(P＞0.05).However,at 1 year of follow-up,the eGFR level in the COVID-19 group was significantly lower than that in the control group(P＜0.05).The mixed-effects linear model analysis revealed a decrease in eGFR by 1.84 mL/(min·1.73 m2)within 1 year of COVID-19 onset in the COVID-19 group,which inclined 4.62％from baseline,with the most significant decline(7.45％)observed among diabetic patients.Conclusion Patients with CKD who have not undergone renal replacement therapy experience a substantial decline in eGFR and worsened renal function within 1 year of COVID-19,necessitating close long-term renal function mo-nitoring and timely interventions.

Objective:To introduce and validate the Pediatric Nausea Assessment Tool (PeNAT) and the Baxter Retching Faces Scale (BARF) in the assessment of chemotherapy induced nausea in Chinese children with malignant neoplasms, and to explore the cut-off value for rescue antiemetic.Methods:A prospective descriptive study was conducted, 244 children in Sun Yat-sen University Cancer Center with malignant neoplasms who received chemotherapy were selected by convenience sampling from July to August 2021. PeNAT, BARF, Visual Analogue Scale (VAS) and the Faces Pain Scale-Revised(FPS-R) were used to assess the severity of nausea and pain before and after chemotherapy, before and 30-60 minutes after the use of rescue antiemetic or analgesic. After chemotherapy, the children also were asked the changes of nausea severity and whether antiemetic was needed.Results:A test-retest reliability was conducted on the patients with the same severity of nausea before and after chemotherapy, and the intraclass correlation coefficient of the PeNAT and BARF were 0.940 (both P<0.05). After chemotherapy, the PeNAT and BARF were 1.5(1.0, 2.0) and 2.0(0, 2.0) points, which were significantly higher than the 1.0(1.0, 1.0) and 0(0, 0) points before chemotherapy ( Z = - 9.19, - 9.09, both P<0.01). The PeNAT and BARF of 11 cases receiving antiemetic before medication were 4.0 (4.0, 6.0) and 3.0(2.0, 4.0) points, which were higher than the 0(0, 2.0) and 1.0(1.0, 2.0) points without antiemetic ( Z = - 4.03, - 3.86, both P<0.05). After chemotherapy, the correlation coefficients between PeNAT or BARF and VAS-nausea were r = 0.933, 0.957 (both P<0.01), and FPS-R were r = 0.192, 0.189 (both P<0.05). After using antiemetic, PeNAT and BARF were 2.0(2.0, 3.0) and 2.5(2.0, 4.0) points, which were significant different than the 3.0(3.0, 3.8) and 4.0(4.0, 8.0) points before using antiemetic ( Z = - 2.97, - 2.83, both P<0.05). According ROC curves and cut-off values, it was determined that PeNAT≥3 and BARF≥4 had clinical significance and require clinical intervention. Conclusions:PeNAT and BARF have excellent reliability and validity in the assessment of chemotherapy induced nausea in children with malignant neoplasms, they can effectively identify the requirement of rescue antiemetic, and evaluate the efficacy of antiemetic.