OBJECTIVETo investigate the effect of celecoxib on adhesion, invasion, migration and matrix metalloproteinase-2(MMP-2) expression of tongue squamous cell carcinoma cell line Tca8113 cells.

METHODSFollowing incubation with celecoxib, the Tca8113 cells were detected for cell adhesion and migration using cell adhesion assay and Boyden chamber invasion assay. The expression of cyclooxygenase-2 (Cox-2) protein in Tca8113 cells was tected with SP immunohistochemistry staining. The MMP-2 level in supernatant was detected with enzyme-linked immunosorbent assay. RESULTS; The adhesion and Boyden chamber invasion assays showed that, after treatment celecoxib, the ability of adhesion and migration of Tca8113 cells was significantly inhibited. Celecoxib could decrease the expression of Cox-2 protein in Tca8113 cell and decrease the MMP-2 level in supernatant.

CONCLUSIONCox-2 inhibitor celecoxib can significantly inhibit the adhesion and migration of Tca8113 cells. The inhibitory effect on hesion and migration may be correlative with its effect on decrese of Cox-2 protein expression and secretion MMP-2 in Tca8113 cells.

Carcinoma, Squamous Cell ; Celecoxib ; Cell Adhesion ; Cell Line, Tumor ; Cyclooxygenase 2 Inhibitors ; Humans ; Matrix Metalloproteinase 2 ; Pyrazoles ; Sulfonamides ; Tongue Neoplasms

OBJECTIVETo investigate the role of COX-2 inhibitor celecoxib in inhibiting the migration of human tongue squamous cell carcinoma Tca8113 cells.

METHODSThe effects of celecoxib on Tca8113 cell migration were tested using scrape motility assay, cell-matrix adhesion assay and Boyden chamber motility assay.

RESULTSFollowing a 24-hour incubation with 10 and 20 micromol/L celecoxib, the migration of Tca8113 cells was significantly decreased (Plt;0.05). Celecoxib treatment for 24 h also resulted in significantly decreased adhesion of Tca8113 cells on Fn-coated surface in a dose-dependent manner.

CONCLUSIONCOX-2 inhibitor celecoxib can inhibit Tca8113 cell migration, the mechanism of which awaits further investigation.

Carcinoma, Squamous Cell ; pathology ; Celecoxib ; Cell Line, Tumor ; Cell Movement ; drug effects ; Cyclooxygenase 2 Inhibitors ; pharmacology ; Humans ; Pyrazoles ; pharmacology ; Sulfonamides ; pharmacology ; Tongue Neoplasms ; pathology