1.Early event-related potential components in face perception reflect the sequential neural activities.
Yuan YANG ; Guan GU ; Hong GUO ; Yi-Hong QIU
Acta Physiologica Sinica 2011;63(2):97-105
Event-related potential (ERP) studies report that early components P120, N170 and VPP are associated with face processing. Several lines of evidence suggest that VPP is the positive counterpart of N170, and they are generated by the same brain sources. However, whether P120 has a negative counterpart and the relations among these early components (i.e. P120, N170, VPP) remain unclear. In this study, the scalp electroencephalogram was recorded when the subjects passively viewed different stimuli, and ERP was calculated. The synchronization of electroencephalography signals between fronto-central and bilateral occipitotemporal sites was evaluated, and independent component analysis was employed to seek the face-sensitive independent components and their corresponding sources. We found that P120 had the negative counterpart, i.e., VN120; moreover, VN120-VPP and P120-N170 complexes were generated by the same sources located in fusiform gyrus, which reflected the same sequential neural activities of face processing.
Adolescent
;
Brain
;
physiology
;
Electroencephalography
;
Evoked Potentials
;
physiology
;
Face
;
Female
;
Humans
;
Male
;
Pattern Recognition, Visual
;
physiology
;
Perception
;
physiology
;
Sensation
;
physiology
;
Young Adult
2.Cerebral pathological evaluation following neural stem cells intraventricular transplantation in neonatal rats with periventricular leukomalacia.
Yue-Qiu HE ; Hui-Jin CHEN ; Long-Hua QIAN ; Guan-Yi CHEN
Chinese Journal of Contemporary Pediatrics 2008;10(3):362-366
OBJECTIVETo evaluate the brain pathological changes following exdogenous neural stem cells (NSCs) intraventricular transplantation in neonatal rats with periventricular leukomalacia (PVL), and to explore the feasibility of NSCs transplantation for the treatment of PVL in premature infants.
METHODSNSCs were prepared from E14 embryonic rat brain. Two-day-old neonatal rats were randomly divided into six groups: PVL, PVL+culture medium, PVL+NSCs, sham operation, sham operation+culture medium, and sham operation+NSCs (18-21 rats each group). Intraventricular transplantation of exdogenous NSCs was performed 72 hrs after PVL induction or sham operation. The cerebral pathological evaluation was undertaken by light microscopy 7, 14 and 21 days after transplantation.
RESULTSThe pathological changes in the cerebral white matter were gradually improved with the prolonged time after transplantation. After 21 days of transplantation, 50% of the cerebral white matter showed mild pathological changes and 50% of that showed severe pathological changes, with neuronal pathological scores of 1.28+/-0.86, in the untreated PVL group. In the PVL+NSCs group, 30% of normal white matter, 40% of mild and 30% of severe pathological changes in the white matter were observed, with neuronal pathological scores of 0.32+/-0.16, 21 days after transplantation. There were very significant differences in both of pathological changes in the cerebral white matter and neuronal pathological scores between the PVL and PVL+NSCs groups (x2=10.7, P<0.01; F=29.664, P<0.01).
CONCLUSIONSIntraventricular transplantation of exdogenous NSCs can apparently improve cerebral white matter damage. It is suggested that intraventricular transplantation of NSCs is of a great potential feasibility for the treatment of PVL in premature infants.
Animals ; Animals, Newborn ; Brain ; pathology ; Female ; Humans ; Infant, Newborn ; Leukomalacia, Periventricular ; pathology ; therapy ; Neurons ; cytology ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Stem Cell Transplantation
3.Study on the sexual development of adolescent male.
Ling GAI ; Dan-Tong YANG ; Hui-Qing SUN ; Guan-Zhao XU ; Yi QIU ; Li-Feng ZHANG ; Zheng-Da REN
National Journal of Andrology 2002;8(5):353-366
OBJECTIVESThe investigation of the testicular volume, the penis length and the T, FSH, LH, PRL levels in serum were taken in 289 adolescent males to provide the valuable data for andrology.
METHODSThe adolescent males were grouped according to their age. The testicular volume was measured with testicular model and the T, FSH, LH, PRL levels in serum were determined by immunoenzymetric assay.
RESULTSThe male sexual development was rapid from age 11 to 16 and close to that of adult at age 18. Serum PRL of adolescent males was higher than that of adult males.
CONCLUSIONSThe age 11 to 16 is a period of rapid growth in sexual maturation. PRL may play an important role in sexual maturation.
Adolescent ; Body Height ; Body Weight ; Gonadal Steroid Hormones ; blood ; Humans ; Male ; Penis ; physiology ; Testis ; physiology
4.Establishment of a neonatal rat model of periventricular leukomalacia and its concomitant cataract.
Yue-Qiu HE ; Hui-Jin CHEN ; Long-Hua QIAN ; Guan-Yi CHEN
Chinese Journal of Contemporary Pediatrics 2007;9(3):220-224
OBJECTIVETo establish a reliable neonatal rat model of periventricular leukomalacia (PVL) which is expected to be similar to PVL of human preterm infants pathologically, and to explore the concomitant eye lesions in the PVL model.
METHODSTwo-old-day neonatal rats were randomly divided into a PVL group and a sham-operated group (n=19 each). The PVL model was established by the ligation of bilateral common carotid arteries, followed by a 30-min exposure to 8% oxygen. The cerebral infarction area was assessed with TTC staining 1 day after operation. Cerebral pathology was examined under a light micsrocope 2 and 21 days after operation. The examinations of eyes under a slip lamp and the pathology of eyeballs under a light microscope were performed 21 days after operation.
RESULTSThe TTC staining cerebral slices showed there were extensive white areas of infarction in the brain of the PVL group, with an infarction area of 53.45 +/- 33.90 mm3 and a percentage of infarction of (24.98 +/- 15.44)% . Significant cystic necrosis and apoptosis around the periventricular and subcortical white matter and mild damage in cortical neurons were observed in the PVL group 2 days after operation. The more obvious cystic necrosis around the periventricular area was found in the PVL group 21 days after operation. There were no pathological changes in the brain of the sham-operated group. All of rats in the PVL group had bilateral cataracts, however, no pathological changes were observed in their postbulbar tissues. The sham-operated group did not show eye abnormal.
CONCLUSIONSThe PVL animal model that was similar to PVL of human preterm infants pathologically was successfully established by the ligation of bilateral common carotid arteries, followed by 30-min hypoxia exposure, with a positive effect and a good repeatability. Cataract can also be induced by the method.
Animals ; Animals, Newborn ; Brain ; pathology ; Cataract ; etiology ; pathology ; Disease Models, Animal ; Female ; Humans ; Hypoxia-Ischemia, Brain ; complications ; Infant, Newborn ; Leukomalacia, Periventricular ; etiology ; pathology ; Male ; Rats ; Rats, Sprague-Dawley
5.Research status on the mechanism of drug resistance in pancreatic cancer
Wan-Qin CHEN ; Qiu-Tong GUAN ; Xiao-Xian HUANG ; Min-Jie WEI ; Zhen-Hua LI
The Chinese Journal of Clinical Pharmacology 2024;40(3):454-458
Objective Pancreatic cancer(PC)is a malignant tumor of the digestive tract that is difficult to diagnose early,easily metastasizes and relapses,and resistant to conventional chemotherapy.PC is a very difficult disease to treat.The key regulatory factors of PC resistance,such as epithelial-mesenchymal transition phenotypic cells,tumor stem cells,and miRNAs,have been reviewed in the past few years,and some new regulatory factors have been discovered as supplements.This review mainly focuses on the characteristics and properties of the key regulatory factors of PC chemotherapy resistance including long noncoding RNAs,nuclear factor KB and exosomes,drug resistance mechanisms,and treatment related strategies,and future treatment directions were predicted.
6.Analysis of phenotype and genotype in three Chinese pedigrees with inherited dysfibrinogenemia.
Qi OUYANG ; Qiu-lan DING ; Dan-dan HUANG ; Guan-qun XU ; Li-wei ZHANG ; Jing DAI ; Ye-ling LU ; Xue-feng WANG ; Xiao-dong XI ; Hong-li WANG
Chinese Journal of Hematology 2011;32(3):153-157
OBJECTIVETo analyze the phenotype and genotype in three Chinese pedigrees with inherited dysfibrinogenemia.
METHODSLaboratory tests including activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT), reptilase time (RT), and the activities of antithrombin (AT:C), protein C (PC:C) and protein S(PS:C) were detected in three pedigrees. The activity and antigen of plasma fibrinogen (Fg) were analyzed by Clauss and immunoturbidimetry methods, respectively. The Fg of three probands was assessed by Western blot and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The sequences of all the exons and exon-intron boundaries of the three Fg genes FGA, GFB and FGG were amplified by PCR and analyzed by direct sequencing.
RESULTSThree probands had normal APTT, PT, PC:C, PS:C and AT:C, but prolonged TT and RT. The activity levels of the 3 probands's plasma Fg were reduced, but antigen levels were normal. Western blot and SDS-PAGE showed no abnormal molecular weight of Fg. The 3 heterozygous mutations of γ Arg275His, Aα Pro18Leu and Aα Arg16Cys were identified in the 3 probands, respectively.
CONCLUSIONThe three probands with dysfibrinogenemia were caused by the mutations of γ Arg275His, Aα Pro18Leu and Aα Arg16Cys, respectively. Both Aα Pro18Leu and Aα Arg16Cys were first reported in Chinese population.
Adult ; Afibrinogenemia ; genetics ; Asian Continental Ancestry Group ; genetics ; Base Sequence ; Female ; Fibrinogen ; genetics ; Genotype ; Humans ; Middle Aged ; Mutation, Missense ; Pedigree ; Phenotype
7.Genotype and function analyses of four inherited dysfibrinogenemia pedigree caused by Arg16 amino acid substitution in fibrinogen Aα chain.
Lin-lin JIANG ; Xue-feng WANG ; Qiu-lan DING ; Guan-qun XU ; Li-wei ZHANG ; Jing DAI ; Ye-ling LU ; Xiao-dong XI ; Hong-li WANG
Chinese Journal of Hematology 2012;33(6):475-479
OBJECTIVETo analyze the phenotype, genotype and function in four Chinese pedigrees with inherited dysfibrinogenemia.
METHODSRouting tests including activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT), reptilase time (RT), the activities of antithrombin (AT), protein C (PC) and protein S (PS) were detected in four pedigrees. The activity and antigen of plasma fibrinogen were analyzed by Clauss and immunoturbidimetry methods, respectively. The molecular weight of fibrinogen of four probands was assessed by Western blot. The function of abnormal fibrinogen was evaluated by fibrinogen clottability, fibrinogen dynamic polymerization and fibrinolysis velocity, respectively. The sequences of all the exons and exon-intron boundaries of the three fibrinogen genes were amplified by PCR and analyzed by direct sequencing.
RESULTSFour probands had prolonged TT and RT, reduced plasma fibrinogen activity levels and normal antigen levels. The assays of Western blot showed no abnormal molecular weight of fibrinogen. Function tests revealed reduced fibrinogen clottability, delayed and decreased fibrinogen dynamic polymerization and reduced fibrinolysis velocity. Aα chain Arg16His and Arg16Cys mutations were identified in the four probands, respectively.
CONCLUSIONThe four probands with dysfibrinogenemia were caused by the mutations of Aα chain Arg16His or Arg16Cys. Mutation of the fibrinogen induced dysfunction of plasma fibrinogen.
Adult ; Afibrinogenemia ; blood ; genetics ; Blood Coagulation Tests ; Female ; Fibrinogen ; genetics ; Fibrinogens, Abnormal ; genetics ; Genotype ; Humans ; Male ; Middle Aged ; Pedigree ; Phenotype ; Thrombin Time
8.Molecular mechanisms of recurrent venous thrombosis in two pedigrees with type I antithrombin deficiency.
Yan XIA ; Qiu-lan DING ; Guan-qun XU ; Li-wei ZHANG ; Jing DAI ; Ye-ling LU ; Xue-feng WANG ; Xiao-dong XI ; Hong-li WANG
Chinese Journal of Hematology 2011;32(12):848-853
OBJECTIVETo investigate the clinical phenotype, genotype and molecular mechanism of recurrent venous thrombosis in two Chinese pedigrees with type I antithrombin (AT) deficiency.
METHODSThe routine coagulation screening tests were detected, thrombin generation tests was performed to evaluate the hypercoagulation. Anticardiolipin antibody (ACA) and lupus anticoagulant (LA) were detected with enzyme-linked immunosorbent assay (ELISA) and diluted viper venom time assay (DVVT), respectively. The activities of protein C, protein S and AT (PC:A, PS:A, AT:A) were tested with chromogenic substrate assay or clotting method. The antigen of AT (AT:Ag) was performed with immunoturbidimetry methods. Western blot was used to analyze the molecular weight (MW) and the plasma levels of AT:Ag. All 7 exons and the flanking sequences were amplified by PCR. The mutation of AT gene and thrombophilia associated gene polymorphisms were analyzed by direct DNA sequencing. The expression plasmid of Ala404Asp mutant was constructed with site-directed mutagenesis method based on the wild-type (WT) AT cDNA contained in pcDNA 3.1 vector, and transiently expression of AT WT and the Ala404Asp mutant was performed using HEK293T cells. Cultured supernatant and cell lysates were collected and measured for AT:Ag by ELISA and Western blot.
RESULTSThe results of routine coagulation tests in two probands were normal, thrombin generation tests indicated that proband 1 presented hypercoagulable state with 2.8 and 1.5 times higher of the endogenous thrombin potential (ETP) and peak height compared with that of normal, respectively. The levels of PC:A, PS:A, ACA and LA were normal. AT:A in proband 1 and proband 2 were 45% and 32%, and AT:Ag were almost half of the normal (121 mg/L and 158 mg/L), respectively. The results of Western blot showed that both probands' plasma levels of AT:Ag were lower than the normal pooled plasma and MW was normal. Two heterozygous mutations of g.3291C→T(Thr98Ile), g.13863C > A(Ala404Asp) were identified in the probands, respectively. No proband had venous thrombosis associated gene polymorphisms. Expression in vitro showed that AT:Ag in culture media and lysates of Ala404Asp are 4.8% and 60.6% of that of WT, respectively.
CONCLUSIONThr98Ile and Ala404Asp mutation of AT gene significantly correlate with recurrent venous thrombosis in the two probands, respectively. Ala404Asp has not been described before. The mutant Ala404Asp protein can not be expressed due to impaired secretion and increased intracellular degradation, resulting in type I AT deficiency.
Adult ; DNA Mutational Analysis ; Female ; Fibrin ; deficiency ; genetics ; Humans ; Middle Aged ; Mutation ; Pedigree ; Phenotype ; Venous Thrombosis ; genetics
9.Transcatheter coil embolization of multiple coronary artery-to-left ventricle fistulas: report of a rare case.
Ruo-gu LI ; Wei-yi FANG ; Hong-yu SHI ; Xin-kai QU ; Hui CHEN ; Xin-biao QIU ; Ying-jia XU ; Jia-lin DONG ; Shao-feng GUAN ; Bei JIANG ; Wei-hua WU
Chinese Medical Journal 2008;121(14):1342-1344
10.Effect of two kinds of fermented food on blood glucose and lipids in induced diabetic mice.
Yong-Qiu YAN ; Guan-Hai DAI ; Shi-Ping JIANG ; Yu LU ; Ying-Peng TONG ; Ping WANG
Chinese Journal of Applied Physiology 2018;34(2):130-133
OBJECTIVES:
To investigate the effect of two kinds of fermented food, noni juice and natto, on blood glucose and lipids in induced diabetic mice.
METHODS:
Female (ICR) mice were induced into diabetes by an injection of alloxan (55 mg/kg, i. v.). After 72 hours, those mice whose fasting blood glucose levels were over 12.00 mmol/L and urine sugar was strongly positive (+ + +) were regarded as diabetical model and were randomly divided into three groups (=10):diabetical model (DM) group, noni juice (NJ) group and natto (NT) group. Another ten normal female ICR mice were taken as normal control (NC) group. The mice in NJ and NT groups were gavaged with noni juice (25.0 ml/kg) and natto (0.6 g/kg) respectively. The other two groups were given normal saline (25.0 ml/kg). Continuous gavage administration was given for 30 days, the water-drinking volume and food-intake were recorded. After 1.5 h of the last administration, the glucose tolerance of mice was measured. Finally, the changes in glycated serum protein(GSP), insulin(Ins) and blood lipids of blood samples of mice, taken from the femoral artery, were determined.
RESULTS:
Compared to the NC group, the water-drinking amount and food-intake, GSP and total cholesterol (TC), triglyceride(TG) and low density lipoprotein (LDL) in DM group were increased significantly (<0.01), while glucose tolerance, Ins and high density lipoprotein (HDL) were decreased significantly (<0.01). However, when it came to DM group, NJ and NT could significantly (<0.01 or <0.05) reduce, GSP, TG and LDL, meanwhile improve glucose tolerance, Ins and HDL (<0. 01).
CONCLUSIONS
Both noni juice and natto could reduce the blood glucose levels in induced diabetical mice and improve blood lipids, which suggested that they may have certain application value in prevention and treatment of diabetic mellitus.
Animals
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Blood Glucose
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Diabetes Mellitus, Experimental
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blood
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Female
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Fermented Foods
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Fruit and Vegetable Juices
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Lipids
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blood
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Mice
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Mice, Inbred ICR
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Random Allocation