Objective: To analyze the clinical data of 153 patients with novel coronavirus pneumonia (COVID-19) in chongqing ,and provide reference and thinking for the diagnosis and treatment. Methods: Analyze the clinical data, laboratory examination and chest imaging characteristics of 153 COVID-19 patients in Chongqing Public Health Medical Center from January 26 to February 5, 2020. According to the relevant diagnostic criteria ,patients were divided into non-severe group(n=132) and severe group(n=21),and analyze the correlation between serum index changes and disease severity. Results: Combined with diabetes and chronic respiratory diseases, the severity of the disease was statistically significant (χ²=11.04和6.94, P<0.05). No symptoms were found in patients with mild illness (χ²=4.09, P<0.05) .The proportion of fever and muscle soreness in the severe group was higher than that in the non-severe group (χ²=4.40 and 22.67,P<0.05).Among the concomitant symptoms, the proportion of cough and shortness of breath in the severe group was higher than that in the non-severe group (χ²=8.46 and 4.80,P<0.05).C-reactive protein and d-dimer were higher in the severe group than in the non-severe group (t=43.44 and 37.13, P<0.05), and the number of CD₃⁺T lymphocyte cells, CD₄⁺T lymphocyte cells and CD₈⁺T lymphocyte cells in the severe group was lower than that in the non-severe group (Z=27.25, 20.60 and 17.36, P<0.05).Compared with the non-severe group, both lungs and the right lung lower lobe were more susceptible to involved (χ²=6.95和20.39, P<0.05) . Conclusion Severity of COVID-19 was associated with underlying disease, symptoms, site of involvement, C-reactive protein, d-dimer, CD₃⁺T lymphocyte count, CD₄⁺T lymphocyte count, and CD₈⁺T lymphocyte count. 

Objective To explore the temporal trend of cancer death rates in different age and the influencing factors in Kunshan,Jiangsu province,1981 to 2015.Methods Data were derived from cancer rcgistry and vital registration system.The Chinese age structure in 2000 was used to calculate age-standardized death rates (ASR),and annual percentage changes (APC) and 95％ confidence interval (Cl) were used to estimate the temporal trend of cancer death rates.Difference decomposition method was applied to analyze the contribution of demographic and non-demographic factors for the change of cancer mortality.Results Between 1981 and 2015,the age standardized all cancers death rate decreased from 162.49 to 93.74 per 100,000 (APC=-l.6％,95％ CI:-1.8％--1.5％).However,the ASR for those aged 70 years or above was stable over time (APC=0.2％,95％ CI:-0.2％-0.5％),whereas aged 30-69 years was decreased from 240.01 in 1981 to 93.28 in 2015 (APC=-2.8％,95％ CI:-3.0％--2.6％).In addition,the proportion of leading cancers were changed obviously.The proportion of lung cancer increased from 1981 to 2015,while gastric cancer,liver cancer,esophageal cancer and colorectal cancer decreased.Compared with the crude cancer mortality in 1993,the effect of the demographic and non-demographic factors to the increased death rate in 2015 were 308.93％ and-208.93％,respectively.Conclusion The ASR death rate of all cancers was decreasing,and the rate in those aged 30 to 69 years decreased significantly,whereas stable in those aged 70 years or above.The effect of demographic characteristics on cancer mortality was significantly greater than that of non-demographic characteristics.

Objective:To analyze the clinical features of patients with COVID-19 in Chongqing Municipality.Methods:The clinical data, laboratory tests and chest imaging findings of 153 patients COVID-19 admitted in Chongqing Public Health Medical Center from January 26 to February 5, 2020 were retrospectively reviewed. According to the relevant diagnostic criteria, patients were divided into non-severe group (n=132) and severe group (n=21). The correlation between serum index changes and disease severity was analyzed.Results:The proportion of patients with underlying diabetes or chronic respiratory diseases in severe group was significantly higher than that in non-severe group ( χ2=11.04 and 6.94, P<0.05). The proportion of symptom-free patients in non-severe group was significantly higher than that in severe group ( χ2=4.09, P<0.05). The symptoms of fever, fatigue and muscle soreness in the severe group were more common than those in the non-severe group ( χ2=4.40, 14.42 and 22.67, P<0.05). Among the concomitant symptoms, the proportion of cough and shortness of breath in the severe group was higher than that in the non-severe group ( χ2=8.46 and 4.80, P<0.05). C-reactive protein and D-Dimer levels were higher in the severe group than those in the non-severe group ( Z=-4.39 and -1.96, P<0.05), and the number of CD3 + T lymphocyte cells, CD4 + T lymphocyte cells and CD8 + T lymphocyte cells in the severe group was lower than that in the non-severe group ( Z=27.25, 20.60 and 17.36, P<0.05). Compared with the non-severe group, both lungs and the right lung lower lobe were more susceptible to be involved( χ2=9.71和23.61, P<0.05). Conclusions:There are significant differences in underlying diseases, clinical symptoms, imaging manifestations and laboratory findings between severe and non-severe patients with COVID-19.

Objective To observe the risk factors and prevalence of cardiovascular disease,and predict the 10-year risk of ischemic cardiovascular disease (ICVD) of a rural residents in Xianghe of Hebei province.Methods Two thousand five hundred and thirty two adults (≥ 35 years old) were surveyed at internal medicine outpatient department of Xianghe asthma hospital in Hebei province by face-to-face interview,physical examination and biochemical test.Subjects aged 35 to 59 were also evaluated using the National 10-year Risk Assessment for ICVD.Results The prevalence of stroke and coronary heart disease was 2.2％ (56/2532) and 6.9％ (176/2532) respectively,the age-and sex-standardized prevalence was 1.3％ and 5.9％ respectively.The prevalence of hypertension,diabetes,dyslipidemia,overweight,obese and central obesity was 59.9％ (1516/2532),26.9％ (682/2532),68.5％ (1735/2532),40.9％ (1038/2532),14.8％ (374/2532) and 49.5％ (1254/2532) respectively,the age-and sex-standardized prevalence was 43.8％,19.9％,56.5％,35.1％,15.6％,41.9％,respectively.Ten-year ICVD risk was higher than 10％ in 14.1％ (188/1336) residents aged between 35 to 59 years.Conclusions Risk factors and prevalence of cardiovascular disease as well as 10-year risk of ICVD are high in this rural population in Xianghe of Hebei province.Intensive prevention and therapy strategies are urgently needed to attenuate the ICVD risk factors and treat ICVD in rural area of China.

Objective To evaluate the effect of pain management training to junior nurses' practice of pain assessment,to provide evidence for continuing education of pain management.Methods 60 junior nurses were randomly assigned to experimental group(30 cases) and the control group (30 cases).Nurses in control group received convention education,whereas nurses in experimental group received a pain management training for four weeks.The practice of pain assessment of two groups were compared after 4-week training.Results After training,the knowledge and attitude of pain management of nurses in experimental group scored an average of (29.20 ± 3.25),whereas the average score of nurses in control group is (16.83 ± 2.87).There was significance difference between two groups (t =-15.625,P ＜ 0.01) ; in terms of the contents of pain assessment,nurses in experimental group were more sufficient and more accurate (t =-14.726,P ＜ 0.01).Regarding the practice of pain assessment the mean score of nurses in experimental group and control group were (6.70±1.47) and (2.93 ±0.87).There was significant difference between two groups (t =12.11,P ＜0.01).For using pain assessment tool,the nurses in experimental group get 100.0％ correct rate,whereas nurses in control group get 30.0％ correct rate,which shows significant differences statistically (x2 =27.81,P ＜ 0.01).Conclusions The pain management training can improve nurses' pain knowledge,attitude,as well as promote the accuracy of nurses 'assessment practice consequently.

With the increase of elderly population, more and more implant operations need to be performed in osteoporotic bone, while different forms of microdamage will be produced in peri-implant bone intraoperatively, including high- and low-density diffuse damages, as well as linear cracks. The length and location of the microcracks are the main factors in affecting the biomechanical performance of bone. Suppression of bone remodeling by bisphosphonates may lead to microdamage accumulation, which is often accompanied with the decrease of bone strength and the increase of bone fragility. Microdamage can be repaired by bone remodeling or mineralization to maintain the strength and structural integrity. Both remo- deling and mineralization can affect the bone quality and long-term implant stability. In this paper, we make a brief summary of some important issues and research progresses in this field.
Bone Remodeling ; Humans

OBJECTIVETo explore the overlap ratio of the target volume in different respiratory statuses of active breath control (ABC) and their differences during external-beam partial breast irradiation (EB-PBI), and from the perspective of target volume overlap to determine the influence of the ABC-assisted breathing condition on intra-fractional target movement of EB-PBI.

METHODSThe patients, who received breast-conserving surgery with silver clips marked at the margins of the cavity and were suitable for EB-PBI, were immobilized on the breast bracket to undertake CT simulation assisted by ABC device, six sets of CT simulation images including two sets of image in state of moderate deep inspiration breathing control (mDIBH), two sets of images in state of free breath (FB) and two sets of images in state of deep expiration breathing control (DEBH) were obtained. The six sets of images were transferred to Pinnacle(3) treatment planning system (TPS), then automatic fusion and registration between two sets of mDIBH images, two sets of FB images, two sets of DEBH images and mDIBH image and DEBH image were achieved separately. Thereafter, the overlap ratios of GTV with GTV, CTV with CTV, PTV with PTV were calculated by the Pinnacle(3) TPS. The differences between the overlap ratios of the three kinds of targets in the same registered image and the difference between the overlap ratios of the same kind of target in the different registered images were statistically analyzed using statistical package of SPSS 11.5.

RESULTSBased on mDIBH/mDIBH registration, the overlap ratios of GTV/GTV, CTV/CTV and PTV/PTV were (83.54 ± 11.41)%, (93.00 ± 6.49)%, and (95.26 ± 4.90)%, respectively, and the differences of the overlap ratios between GTV/GTV and CTV/CTV, GTV/GTV and PTV/PTV were all statistically significant (P < 0.05), but statistically not significant between CTV/CTV and PTV/PTV (P > 0.05). Based on FB/FB registration, the overlap ratios of GTV/GTV, CTV/CTV and PTV/PTV were (72.55 ± 29.10)%, (89.36 ± 9.53)% and (92.47 ± 7.25)%, respectively, and the differences of the overlap ratios between GTV/GTV and CTV/CTV, CTV/CTV and PTV/PTV were all not statistically significant (P > 0.05), but statistically significant between GTV/GTV and PTV/PTV (P < 0.05). Based on DEBH/DEBH registration, the overlap ratios of GTV/GTV, CTV/CTV and PTV/PTV were (79.48 ± 22.31)%, (92.83 ± 6.77)% and (95.05 ± 4.81)%, respectively, and the differences of the overlap ratios between GTV/GTV and CTV/CTV (P = 0.000), CTV/CTV and PTV/PTV (P = 0.037), GTV/GTV and PTV/PTV (P = 0.000) were statistically all significant (P = 0.000). The differences of the overlap ratios of GTV/GTV, CTV/CTV, and PTV/PTV (P = 0.000) between mDIBH/mDIBH and DEBH/DEBH, mDIBH/mDIBH and FB/FB, FB/FB and DEBH/DEBH were all statistically significant (P = 0.000), and not statistically significant between mDIBH/mDIBH and mDIBH/DEBH, FB/FB and mDIBH/DEBH.

CONCLUSIONSDuring the delivering of EB-PBI assisted by ABC, the intra-fractional overlap ratios of the target volume between the same breathing state is increasing in the order of GTV/GTV → CTV/CTV → PTV/PTV. The difference of the overlap ratios of the target volumes between mDIBH and mDIBH, FB and FB, DEBH and DEBH is not significant, and the overlap ratios of PTV/PTV in the three breathing statuses of mDIBH, FB and DEBH reaches a higher level. Therefore, from the perspective of target volume overlap, if the setup error is corrected online before delivering, the necessity of breathing control during delivering of EB-PBI is worthy discussing.

Breast Neoplasms ; radiotherapy ; surgery ; Dose Fractionation ; Female ; Humans ; Immobilization ; Mastectomy, Segmental ; Postoperative Period ; Radiotherapy, Conformal ; methods ; Respiration ; Tomography, X-Ray Computed

Objective To observe the clinical efficacy and safety of tiotropium bromide powder inhalation combined with salmeterol xinafoate and fluticasone propionate powder inhalation in the treatment of acute exacerbation of chronic obstructive pulmonary disease (COPD) complicated with respiratory failure.Methods Seventy-six patients with acute exacerbation of COPD complicated with respiratory failure were randomly divided into control group and treatment group with 38 cases per group.Control group received routine treatment and ventilator-assisted ventilation treatment.Treatment group received salmeterol xinafoate and fluticasone propionate powder inhalation 1 suction,bid,inhalation treatment +tiotropium bromide powder inhalation 18 μg,qd,inhalation treatment,on the basis of control group.Two groups were treated for 2 weeks.The clinical efficacy,arterial blood gas indexes,procalcitonin,immune functions and adverse drug reactions were compared between two groups.Results After treatment,the total effective rates of treatment and control groups were 92.11％ (35 cases/38 cases) and 73.68％ (28 cases/38 cases) with significant difference (P ＜0.05).After treatment,the main indexes in treatment and control groups were compared:arterial partial pressure of oxygen were (86.35 ±7.04) and (77.49 ±6.85)mmHg,oxygenation index were (310.89 ±29.87) and (281.34 ±27.53)mmHg,procalcitonin were (1.42 ± 0.54) and (1.93 ± 0.61)ng · mL-1,tumor necrosis factor-α were (275.49 ±48.62) and (310.05 ±54.73)ng · L-1,interleukin-8 were (312.62 ±75.64) and (389.75 ±78.76)pg · mL-1,high sensitive C-reactive protein were (4.73 ±2.45) and (8.34 ±2.53)mg · L-1,the differences were statistically significant (all P ＜ 0.05).There were no adverse drug reactions in the two groups during the treatment.Conclusion Tiotropium bromide powder inhalation combined with salmeterol xinafoate and fluticasone propionate powder inhalation has a definitive clinical efficacy and safety in the treatment of acute exacerbation of COPD complicated with respiratory failure,which can significantly reduce the levels of procalcitonin,improve the blood gas indexes and immune function.

OBJECTIVETo study the feasibility of MRI of human colon adenocarcinoma cell line (Lovo) labeled with superparamagnetic iron oxide(SPIO) nanoparticles in vitro.

METHODSLovo cells (5 × 10(5) and 1 × 10(6)) were cultured in medium containing different SPIO nanoparticles (50 microl and 500 microl). Transmission electron microscopy was used to observe cellular ultrastructure and to determine the uptake and distribution of particles in Lovo cells at 1-, 3-, 6-hours. MRI of Lovo cells was performed with T1WI, T2WI sequences. Unlabeled cells were used as controls.

RESULTSUptake of SPIO nanoparticles occurred within 6 hours. On T1 weighted imaging, there was no significant difference in signal intensity between the experimental groups and the control group. On T2 weighted imaging, there was no significant difference in signal intensity between the experimental groups and the control group after culture of 1 h. Signal intensity began to decrease in 1 × 10(6) Lovo cells labeled with 500 microl SPIO nanoparticle after 3 hours culture. Signal intensity decreased in all the experimental groups after 6 hours culture.

CONCLUSIONHuman colon adenocarcinoma cell line (Lovo) can be labeled with SPIO nanoparticles, and the labeled cells can be imaged with MRI equipment.

Adenocarcinoma ; pathology ; Cell Line, Tumor ; Colonic Neoplasms ; pathology ; Humans ; Iron ; Magnetic Resonance Imaging ; methods ; Magnetics ; Nanoparticles ; Oxides ; Pilot Projects ; Staining and Labeling ; methods

Objective:To construct HIV-1 pseudovirus containing enhanced green fluorescent protein ( EGFP ) gene. To understand the interaction between the virus and the cells. Methods: HIV-1 pseudovirus containing EGFP gene was constructed by lentiviral packaging systems, and its EGFP gene was amplified using RT-PCR. The level of genomic integration and transcription of HIV-1 pseudovirus containing EGFP gene were detected on iDCs infected with HIV-1 pseudovirus. At the same time, research on expression of the EGFP gene in iDCs infected with HIV-1 pseudovirus was performed. Results:The EGFP gene of HIV-1 pseudovirus was detected through RT-PCR. The EGFP gene was identified in iDCs infected with HIV-1 pseudovirus through PCR and RT-PCR. The EGFP was observed in iDCs infected with HIV-1 pseudovirus under fluorescence microscopy. Conclusion: HIV-1 pseudovirus containing EGFP gene has been successfully produced. The HIV-1 pseudovirus that we constructed can infect iDCs,then its RNA can integrate into the genome of iDCs in the way of reverse transcription,and the EGFP gene could express in the iDCs after infected with HIV-1 pseudovirus.