Problems such as detached knowing and doing , utilitarian tendency , moral practice ability is weak and so forth are now existing in the medical college students'moral practice in the new period .There are not only medical students'personal factors , but also school and social factors .Therefore , it should excavate potential , build good medical students moral practice platform , innovative form of moral practice , motivate medical students to par-ticipate in the moral practice enthusiasm , strengthen the construction of management system , ensuring the medical students moral practice into effect .

Objective To compare the clinical-pathology features between male breast cancer ( MBC) and female breast cancer(FBC)patients.Methods Clinical data of 37 MBC and 300 FBC patients admitted in the First Affiliated Hospital of Zhengzhou University from Jun .2003 to Jun.2013 was retrospectively reviewed . Results The ratio of MBC to FBC was 54:10000.The age of MBC patients ranged from 39 to 80 years with the median age of 62 years old, older than FBC patients(t=3.117, P=0.002).The percentage of invasive ducal carcinoma was much higher in MBC patients than in FBC patients (χ2 =7.535,P=0.023).MBC patients in clinically stage III and IV were more than FBC patients (χ2 =16.471,P=0.000).The positive rate of estrogen receptor(ER)was significantly higher in MBC patients than in FBC patients (χ2 =6.294,P=0.043).The posi-tive rate of human epidermal growth factor receptor-2 ( HER2 ) was lower in MBC patients than in FBC patients (χ2 =11.222,P=0.004).The main surgical method of MBC was modified radical mastectomy , and there was no breast-conserving surgery for MBC, while the surgical method of FBC was various (χ2 =7.720,P=0.021). Conclusion There are significant differences between FBC and MBC patients in clinicopathological features , surgical method and even prognosis , which were important to guide the treatment of MBC and FBC .

OBJECTIVE:To establish a method for determination of Morphine in Pipazhike soft capsules by using SPE-HPLC.METHODS:SPE condition:C18 cartridge.15mL MeOH-water(3∶ 1)and 5mL water were used as elution solvent.RESULTS:Morphine had a good linearity in the rang of 0.233 5～ 1.868 0? g(r=0.999 9).The average recovery rate was 97.6%(RSD=1.2%,n=5).CONCLUSION:This method is simple,accurate,and reproducible,and can be used to effectively control the content of Morphine in Pipazhike soft capsules.

Azone. PG combined with azone showed a higher synergic effect of enhancing effectiveness. Conclusion National Starch polyacrylate pressure-sensitive adhesive was the most effective pressure-sensitive adhesive matrix, PVP-K30 was the most effective crystallization inhibitor, PG combined with azone were the most effective penetration enhancers.

IPA.Skin permeation was significantly enhanced by increasing the loading concentration of OA or NMP,larger IPA amount(8%) or PG amount(20%) could inhibit the permeation of artesunate.Conclusion Oleic acid was found to be the most efficient enhancer for artesunate,10% is the most effective concentration.

0.05).Conclusions Serum DAO can be used as a sensitive marker to early diagnose gastrointestinal dysfunction in critical neonates.Serum D-lactate will be of high specific clinical significance in diagnosis,the change of D-lactate may have relations to the features of intestinal microflora in early neonates.

Objective To determine if fetal stem cells can enter the maternal circulation during pregnancy and re-pair the injuries of maternal heart.Methods C57 female mice at the age of 6-8 weeks were randomly assigned to three groups:sham control, surgery without pregnancy, and surgery with pregnancy ( n=8,eath group) .The control sham group was developed by opening and closing of the chest.The other two groups underwent heart surgery.The myocardial infarc-tion ( MI) model was induced by ligation of the left anterior descending coronary artery.Half of the surgical mice mated with e-GFP transgenic male mice, and another half group was not.Electrocardiogram ( ECG) and echocardiographic images were recorded at pre-operation, post-operation and postpartum.The collected data were used to evaluate the heart function. The GFP expression was detected by immunohistochemistry and q-PCR.Results When compared with the sham group, both the ischemia surgery groups with and without pregnancy, the ECG ST segment was significantly increased.This meas-urement indicated that the myocardial ischemia surgery was successful, and no significant difference in the ST segments be-tween two ischemia surgery groups was found.However, when ECG was measured in the surgical mice after postpartum, their myocardial ischemia was dramatically improved when compared with that of the ischemia surgery only mice.Echocar-diographic images also indicated that both the surgery groups had myocardial ischemia, however, no significant difference was observed in the pregnant mice before and after postpartum.The order of the cardiac function indexes from high to low was the sham group, surgery with pregnancy group, and surgery with no pregnancy group;in particular, the cardiac func-tion of pregnancy group was significantly enhanced compared with that of the surgery with no pregnancy group (P<0.05). More importantly, both immunofluorescence and q-PCR results showed that the embryonic stem cell translocation through circulation system with GFP expression in the heart of pregnancy group, while negative in other two groups.Conclusions Embryonic stem cells can be transferred into the maternal circulation of pregnant mice, and play a role in the repairing of their cardiac injuries.

AIM:To investigate the effects of 17β-estradiol on the expression of macrophage migration inhibi-tory factor ( MIF) in cultured endometrial stromal cells from endometriosis .METHODS:Immunohistochemistry was used to identify the endometrial stromal cells .The expression of MIF at mRNA and protein levels was assessed by RT-PCR and Western blotting .RESULTS:Elevated expression of MIF at mRNA and protein levels was observed in the cultured endom-etrial stromal cells treated with 17β-estradiol.In endometrial stromal cells from the women with endometriosis , the level of MIF up-regulation by 17β-estradiol was significantly higher than that in the cells from the women without endometriosis . CONCLUSION:Endometrial stromal cells from endometriosis are more sensitive to 17β-estradiol, which up-regulates the expression of MIF and contribute to the pathogenesis and progression of endometriosis .

Objective To summarize the clinical efficacy of Millikan's modified modality using tension-free mesh-plug inguinal herniorrhaphy. Methods A retrospective study was performed in 185 cases with in-guinal hernias. They received surgical treatment using Millikan's modality in our hospital from Jan. 2005 to Dec. 2006. Results There were 184 males and 1 female in these patients with a average age of 47 years ( range 32 - 75 years). Among them, 7 cases had bilateral hernia. The mean operative time of each hernia was 49 min (range 30 -70 min), and the average postoperative hospital stay was 5. 1 days (range 3 - 18 d). The complication rate was 10. 8% (20/185). All patients had no recurrence after following-up over 24 months. Conclusion Millikan's modified mesh-plug hemioplasty is a safe and effective modality in the pri-mary inguinal hernia repair, and has fewer complications and lower recurrence rate.

Objective To investigate the protection effect of dexmedetomidine against H2O2 injury in Human renal tubular epithelial cells(HK-2 cells). Methods HK-2 cells cultured in vitro were randomly divided into four groups(n = 24): control group, dexmedetomidine pretreatment group, H2O2 injury group, H2O2 injury +dexmedetomidine pretreatment group. Cell viabilities were measured by MTS assay, cell apoptosis were detected using flow cytometry, and expression of HIF-1α protein was quantified by western blot. HK-2 cells were divided into 8 groups by combining with three treatment factors such as PI3K inhibitor LY294002, dexmedetomidine and H2O2 injury. MTS assay was used to detect cell viability and western blot was used to quantify protein expression of HIF-1α,Bcl-2 and Bax after treatment in each group. Results Dexmedetomidine significantly increased the level of HIF-1α、 Bcl-2 in HK-2 cells after H2O2 injury, thus improved viabilities and reduced apotosis of cells. Moreover, effect on H2O2 injury cells of Dexmedetomidine was reversed by PI3K inhibitor LY294002. Conclusion Dexmedetomidine could protect against H2O2 injury by up-regulating HIF-1α expression through activating PI3K/Akt/mTOR signaling pathway in HK-2 cells.