Objective To analyse the criterion-related validity of comprehensive ICF Core Sets for stroke. Methods Comparison of concepts of items among SF-36, Barthel index, Mini-mental State Examination, WHO quality of life-BREF, WHO Disability Assessment Scale Ⅱ and Comprehensive ICF Core Sets for stroke had been conducted using the linking rules established by Cieza and Stucki. Field test had been conducted for 93 patients with stroke with the above 6 scales. Results 16 body function items and 27 activities and participation items matched with items of the selected functioning measurement scales (0.4

Objective To assess the courses of evidence-based medicine among graduate students. Methods A sample of 118 graduate students who took the course were surveyed twice by using the same self-designed questionnaire at the beginning and the end of the course.Pre-post comparison was conducted to measure the impact. Results The students were able to grasp the most of the contents of the course,and reshaped their opinions on evidence-based medicine,with which,a positive impact on their medical practice was expected. Conclusion The course of evidence-based medicine for graduate students has resulted in a positive consequence.

To study the antiarrhythmic effect of the newly developed alpha/beta-blocker TJ0711, a variety of animal models of arrhythmia were induced by CaCl2, ouabain and ischemia/reperfusion. Glass microelectrode technique was used to observe action potentials of right ventricular papillary muscle of guinea pig. The onset time of arrhythmia induced by CaCl2 was significantly prolonged by TJ0711 at 0.75, 1.5 and 3 mg x kg(-1) doses. TJ0711 (1.5 and 3 mg x kg(-1)) can significantly shorten the ventricular tachycardia (VT) and ventricular fibrillation (VF) duration, the incidence of VF and mortality were significantly reduced. On ischemia-reperfusion-induced arrhythmic model, TJ0711 (0.25, 0.5, 1 and 2 mg x kg(-1)) can significantly reduce the ventricular premature contraction (PVC), VT, VF incidence, mortality, arrhythmia score with a dose-dependent manner. At the same time, rats serum lactate dehydrogenase (LDH) and creatine kinase (CK) activities decreased significantly by TJ0711 (1 and 2 mg x kg(-1)). Ouabain could cause arrhythmia in guinea pigs, when TJ0711 (0.375, 0.75, 1.5 and 3 mg x kg(-1)) was given, the doses of ouabain inducing a variety of arrhythmia PVC, VT, VF, cardiac arrest (CA) were significantly increased with a dose-dependent manner. In the TJ0711 0.1-30 micromol x L(-1) concentration range, guinea pig right ventricular papillary muscle action potential RP (rest potential), APA (action potential amplitude) and V(max) (maximum velocity of depolarization) were not significantly affected. APD20, APD50 and APD90 had a shortening trend but no statistical difference with the increase of TJ0711 concentration. TJ0711 has antiarrhythmic effect on the sympathetic nerve excitement and myocardial cell high calcium animal arrhythmia model. Myocardial action potential zero phase conduction velocity and resting membrane potential were not inhibited by TJ0711. APD20, APD50 and APD90 were shortened by TJ0711 at high concentration. Its antiarrhythmic action mechanism may be besides the action of blocking beta1 receptor, may also have a strong selective blocking action on alpha1 receptor and reducing intracellular calcium concentration.
Action Potentials ; drug effects ; Adrenergic alpha-Antagonists ; administration & dosage ; pharmacology ; Adrenergic beta-Antagonists ; administration & dosage ; pharmacology ; Animals ; Anti-Arrhythmia Agents ; administration & dosage ; pharmacology ; Arrhythmias, Cardiac ; blood ; chemically induced ; etiology ; pathology ; physiopathology ; Calcium Chloride ; Creatine Kinase ; blood ; Dose-Response Relationship, Drug ; Female ; Guinea Pigs ; Heart Ventricles ; cytology ; Lactate Dehydrogenases ; blood ; Male ; Myocardial Reperfusion Injury ; complications ; Myocytes, Cardiac ; drug effects ; physiology ; Ouabain ; Papillary Muscles ; cytology ; Phenoxypropanolamines ; administration & dosage ; pharmacology ; Random Allocation ; Rats ; Rats, Sprague-Dawley

In the study we determined the content of malondialdehyde(MDA) , which is the metabolite of lipid peroxide(LPO) , in plasm and lung homogenate by thiobarbituric acid(TBA) colorimetry, and the content of phosphatidyl-choline(PC), which is the major functional composition of pulmonary surfactant (PS).and total phospholipid (TPL) by thin-layer chromatogra-phy and inorganic phosphorus quantitative analysis in rabbits with lung injury induced by oleic acid. The results showed that the MDA level wasincreased in plasm and lung homogenate; PC and TPL were decreased in lung homogenate. Administrating of 654-2 before and after (0. 5 h)lung injury could both inhibit the production LPO and prevent the decrease of PS. The findings suggest that the decrease of PS may be due to the increase of LPO and that G54-2 could modify the process.

Objective To investigate the effects of OGP (10-14) and its derivate H86A on bone metrology in rats with ovariecto-my-reduced osteoporosis. Methods Thirty-two female Sprague-Dawley rats, three months old, were randomly divided into 4 groups. Rats in groups 1 to 3 were bilaterally ovariectomized(OVX) and the others in group 4 were sham-operated. After the operation, rats in group 1 and group 2 were received derivate of OGP(10-14) H86A daily at different concentration. Group 3 were given placebo. Rats were killed after 12 weeks. Left tibias were disconnected and made into non-decalcified bone slice, and then they were detected and analyzed on indexes which were relative with bone metrology. Results Compared with Group 3, no other indexes except OV/BV was obviously different in rats with high dose H86A (P

Objective To study plasma tumor necrosis factor ?(TNF ?) level and its relationship with insulin resistance in familial type 2 DM. Methods Plasma TNF ? were determined by RIA in 32 familial type 2 DM patients, their 37 non diabetic first degree relatives and 40 healthy control subjects. Results Plasma TNF ? was significantly higher in familial type 2 DM patients[(1.19?0.21)?g/L] than in healthy control subjects[(1.00?0.18)?g/L]( P 0.05). Regression analysis indicated that TNF ? was inversely related to ISI and was a significantly independent contributor to variations in ISI. 39% of the variance in ISI was explained by TC, body mass index(BMI)and TNF ?. Conclusion The finding of an association between high plasma levels of TNF ? and insulin resistance suggests that TNF ? may be involved in the pathophysiology of insulin resistance in familial type 2 DM patients.

Objective To investigate the association of ILK expression in human renal interstitium and the pathological injury of renal interstitium in the patients with chronic nephropathy.Methods The expressions of ILK,TGF?1,E-cadherin and fibronectin(FN) in renal tissue were investigated with immunohistochemistry and computer image analysis system in 49 patients with chronic kidney disease.According to the score of the renal interstitial pathologic injury,49 patients were divided into four groups: 4 in no injury group(the normal control group),17 in slight injury group,16 in mild injury group,12 in severe injury group.Results ILK was hardly detected in normal control group,and ILK was observed in tubulointerstitial area in 3 injury groups.The expression of ILK was prominent in tubular epithelial cells,next in the damaged tubulointerstitial area.A significant positive correlation was found between the expression of ILK in renal interstitial tissue and the degree of renal tubulointerstitial pathological injury(r=0.736),as well as that of TGF?1(P

Objective: To sieve the bioactive constituents of Radix Puerariae,serum pharmacochemistry research was performed.Method: Based on the establishment of HPLC fingerprints of Radix Puerariae,the constituents absorbed into blood were determined by comparing the HPLC fingerprints of the methanol extracts,tested serum samples and blank serum sample.Results: Four compounds absorbed into blood were detected,among which two were original constituents of Radix Puerariae(including puerarin),the other might be metabolites of the original constituents.Conclusion: These four constituents absorbed into blood were possible bioactive components of Radix Puerariae.Further studies on them will help clarify the bioactive constituents and mechanisms of Radix Puerariae.

Objective To explore the influence of Azithromycin on helper T lymphocyte cell(Th)1/Th2 in peripheral blood of children with bronchitic asthma.Methods Twenty-four asthmatic children and 20 healthy children were selected.Peripheral blood mononuclear cells (PBMC) were isolated from venous blood and made into cells suspension in aseptic condition.0.2 mg/L,0.1 mg/L,0.05 mg/L and 0 mg/L Azithromycin were added into the cultures in asthmatic group.The control group was not interfered with Azithromycin.The supernatant was collected after 48 h.The levels of IFN-?,IL-4 and IL-10 in the supernatant were determined by enzyme-linked immunosobent assay(ELISA).SPSS 11.5 software was used to analyze data.Results 1.The level of IL-4 produced from PBMC of asthmatic group was significantly higher than that of control group(P0.05).2.Azithromycin 0.1 mg/L more promoted the secretion of IL-4 than the other 3 concentrations(Pa0.05).3.Azithromycin 0.2 mg/L and 0.1 mg/L more increased the level of IL-10 than the control group(P0.05).Conclusions The routine drug level of Azithromycin(0.1 mg/L) had no effects on the imbalance of Th1/Th2 of asthmatic children,but could modulate the immunological function by up-regulating the level of IL-10.

Background and purpose:Pterostilbene is a natural antioxidant, whose role in retinoblastoma remains unclear. The aim of this study is to probe the effects of pterostilbene on the proliferation, apoptosis and autophagy in retinoblastoma WERI-Rb-1 cell lines.Methods:Cell counting kit-8 (CCK-8) assays were used to analyze the effects of pterostilbene on the proliferation of WERI-Rb-1 cells. Apoptosis rate was determined by Annexin V/PI. Autophagic vacuoles were observed by acridine orange staining. LC3 and P62 protein expressions were determined using Western blot.Results:Pterostilbene significantly inhibited the proliferation of WERI-Rb-1 cells (P<0.01). The cell viability were (93.02±0.47)%, (55.10±2.04)% and (30.33±1.45)% after WERI-Rb-1 cells were treated with 25, 50 and 100 μmol/L pterostilbene for 24 h, and the cell viability were (88.38±3.70)%, (53.37±1.17)%, (29.60±1.05)% after WERI-Rb-1 cells were treated with 50 μmol/L pterostilbene for 12, 24 and 48 h. Pterostilbene induced cell apoptosis (P<0.01), the apoptosis rates of control group, 24 h treated group and 48 h treated group were (4.08±0.79)%, (13.44±2.12)% and (23.49±2.01)%. Pterostilbene induced autophagy of WERI-Rb-1 cells, increased LC3 expression, downregulated P62 expression and increased the number of autophagic vacuoles in WERI-Rb-1 cells (P<0.01). 3-MA and Beclin1 were able to rescue pterostilbene-induced cell death (P<0.01). After 3-MA was used to blunt autophagosome formation, the apoptosis rate markedly decreased in 3-MA+pterostilbene-treated cells compared with cells treated with pterostilbene alone [(12.97±2.09)%vs (8.35±1.11)%], and after siRNA was used to knockdown Beclin1, the apoptosis rate had the same change [(13.80±2.19)%vs (9.62±0.52)%].Conclusion:Pterostilbene can inhibit the proliferation of WERI-Rb-1 cells and induce cell apoptosis via autophagy activation.