OBJECTIVE:To discuss the application of pharmacoeconomics in "Treating Preventable Diseases" in traditional Chinese medicine. METHODS:Based on the brief introduction of pharmacoeconomics and the theory of "Treating Preventable Diseases",and taking preventing cerebral infarction by using drugs for invigorating blood circulation and eliminating stasis as example to analyze the feasibility and significance of pharmacoeconomics in the study of "Treating Preventable Diseases". RESULTS & CONCLUSIONS:The application of pharmacoeconomics in evaluating the theory of "Treating Preventable Diseases" will establish an objective evaluation system of Traditional Chinese Medicine and guide the rational use of drugs in clinic.

Objective To understand the status of indoor air formaldehyde pollution in a certain city in China.Methods 50 newly decorated houses in a certain city were chosen and the formaldehyde concentration in indoor air in different types of room were determined.Results The formaldehyde concentrations in indoor air in 56.6% of the investigated 50 houses exceeded the State Standard.And the highest concentration was 1.05 mg/m3 which was 10.5 times of the limit in the State Standard.Child's bedrooms were most seriously polluted,73% of which exceeded the State Standard.Conclusion The status of indoor air formaldehyde pollution in the newly decorated houses in a certain city in China is serious,this problem needs to be paid more attention to.

Cholangiolocellular carcinoma (CLC) is a rare type of primary liver cancer,which is thought to originate from hepatic progenitor cells.CLC is categorized as a different tumor type from the conventional intrahepatic cholangiocarcinoma (cICC) due to its unique histological and embryological features.This review summarizes the clinical,radiological and pathological characteristics and surgical outcomes of cICC and CLC.This pathological classification may provide important clinical implications for the treatment and outcome evaluation of this disease.

Objective To evaluate the feasibility and indications of endoscopic submucosal dissection (ESD) by analyzing the early gastric cancer cases.Methods All the pathological materials of radical gastric resections in Huashan hospital from 2007 to 2011 were retrieved and the EGC cases were selected.All the cases in EGC group which met the extended ESD criteria were reviewed.Extended ESD criteria were cited from Treatment guidelines for gastric cancer in Japan edited by JGCA in 2001.The risk factors relevant with regional metastasis,such as invasive depth and differentiation degrees,were also analysed.Results In the recent five years,there were 1159 cases of gastric cancer undergoing radical gastrectomy in Huashan hospital including 210 EGC.According to the extended criteria of JGCA,151 cases could be candidates for ESD while 126 cases could be candidates for ESD according to the typical criteria for ESD.The local metastases of lymphatic or vascular involvement for mucosa cancer,muscularis mucosae cancer,sm1 and sm2 cancer were 6.75％,15.00％,23.25％,and 39.62％ respectively (P ＜0.05).Twenty-five cases had local metastases that met the extended criteria for ESD.Within the 210 EGC cases,the local metastases of differentiated type and undifferentiated type were 13.95％ and 47.37％ respectively (P ＜ 0.05).Conclusion Most EGC cases undergoing radical gastrectomy can be cured by ESD.Its metastases are highly correlated with invasion depth and differentiation,so indications of ESD should be assessed.

The human immunodeficiency virus type 1(HIV-1)can interact with and exploit the host cellular machinery to replicate and propagate itself.Numerous studies have shown that the Mitogen-activated protein kinase(MAPK)signal pathway can positively regulate the replication of HIV-1,but exactly how each MAPK pathway affects HIV-1 infection and replication is not understood.In this study,we used the Extracellular signal-regulated kinase(ERK)pathway inhibitor,PD98059,the Jun N-terminal kinase(JNK)pathway inhibitor,SP600125,and the p38 pathway inhibitor,SB203580,to investigate the roles of these pathways in HIV-1replication.We found that application of PD98059 results in a strong VSV-G pseudotyped HIV-1NL4-3 luciferase reporter virus and HIV-1NL4-3 virus inhibition activity.In addition,SB203580 and SP600125 also elicited marked VSV-G pseudotyped HIV-1NL4-3 luciferase reporter virus inhibition activity but no HIV-1NL4-3 virus inhibition activity.We also found that SB203580 and SP600125 can enhance the HIV-1 inhibition activity of PD98059when cells were treated with all three MAPK pathway inhibitors in combination.Finally,we show that HIV-1virus inhibition activity of the MAPK pathway inhibitors was the result of the negative regulation of HIV-1 LTR promoter activity.

Objective To compare the different expressions of transforming growth factor beta1 (TGFβ1) and its type one receptor(TGFβR1) between primary pancreatic cancer cell line AsPC-1 and metastatic pancreatic cancer cell line BxPC-3. Methods The mRNA expressions of TGFβ1 and TGFβR1 in AsPC-1 and BxPC-3 pancreatic carcer cell lines were quantatitived by real-time RT-PCR. The protein levels of TGFβ1 and TGFβR1 in these two cell lines were measured by Western blot assay. Results Compared with the primary pancreatic cancer cell AsPC-1, the mRNA and protein expressions of TGFβ1 and TGFβR1 were much higher in BxPC-3 pancreatic cancer cells(P<0.05). Conclusions Upregulations of TGFβ1 and TGFβR1 might be a pivotal incidence in the procedure of malignant progressing and metastasis in pancreatic cancer cells.

Glucaric acid (GA) is derived from glucose and commonly used in chemical industry. It is also considered as one of the "Top value-added chemicals from biomass" as carbohydrate monomers to produce various synthetic polymers and bioenergy. The demand for GA in food manufacture is increasing. GA has also attracted public attentions due to its therapeutic uses such as regulating hormones, increasing the immune function and reducing the risks of cancers. Currently GA is produced by chemical oxidation. Research on production of GA via microbial synthesis is still at preliminary stage. We reviewed the advances of glucaric acid applications, preparation and quantification methods. The prospects on production of GA by microbial fermentation were also discussed.
Biomass ; Chemical Industry ; Fermentation ; Glucaric Acid ; chemistry ; Glucose ; Polymers

In order to analyze the correlation between critical residues in the catalytic centre of BSH and the enzyme substrate specificity, seven mutants of Lactobacillus salivarius bile salt hydrolase (BSH1) were constructed by using the Escherichia coli pET-20b(+) gene expression system, rational design and site-directed mutagenesis. These BSH1 mutants exhibited different hydrolytic activities against various conjugated bile salts through substrate specificities comparison. Among the residues being tested, Cys2 and Thr264 were deduced as key sites for BSH1 to catalyze taurocholic acid and glycocholic acid, respectively. Moreover, Cys2 and Thr264 were important for keeping the catalytic activity of BSH1. The high conservative Cys2 was not the only active site, other mutant amino acid sites were possibly involved in substrate binding. These mutant residues might influence the space and shape of the substrate-binding pockets or the channel size for substrate passing through and entering active site of BSH1, thus, the hydrolytic activity of BSH1 was changed to different conjugated bile salt.
Amidohydrolases ; genetics ; metabolism ; Bile Acids and Salts ; metabolism ; Escherichia coli ; metabolism ; Gene Expression ; Lactobacillus ; enzymology ; genetics ; Substrate Specificity

Objective To study the renal oncocytoma CT eosinophil performance,improve the rate of correct diagnosis.Methods Retrospective analysis of seven cases confirmed by pathology of renal oncocytoma CT.Results Tumor cortex to the growth mainly three cases to the pelvis grow mainly two cases,two cases are located entirely within the renal parenchyma.Plain;Four cases of tumor performance for the more homogeneous density.Three cases density uniform,which show two cases within Stelletta tenui Lindgren-sharp edge,low-density,one cases show multiple internal circular low-density category,there calcification in 2 cases.Enhanced cortex and medulla period was more homogeneous strengthen four cases,three cases heterogeneous enhance and strengthen below the renal cortex.Pelvis period density decreased,but the level not lower than the same muscle density,and normal kidney tissue clear,two cases showed a complete fake capsule.Conclusions Renal oncocytoma scar except center outside homogeneous enhancement,pelvis with a period of not less than muscle density levels,and normal kidney tissue.Coated with a complete sham and center when scar more precise diagnosis.Other typical performance required when combined with clinical analysis.

Objective To determine the association between fatty liver and coronary risk fcators in elderly men. Methods A cross-sectional study of 920 elderly men with or without fatty liver and obesity was performed in 2002. Age, body mass index (BMI), GPT, cholesterol, triglyceridemia, fast glucose, mean blood pressure, smoking and drinking consumption were analyzed. Results The prevalence of fatty liver was 11.1% in the non-obese participants (BMI