ObjectiveToestablishanionchromatography(IC)methodfordetectionofammoniainthe environmental air of laboratory animal facilities .Methods Dilute sulfuric acid was used as the absorption liquid to collect ammonia in the air.Ammonia was converted into ammonium ion in the absorption solution .With methylene sulfonic acid as the pouring liquid , the content of ammonium ion in the absorption solution was detected , and the concentration of ammonia in the air was calculated.Results The ammonium ion showed good linearity in the range of 0 to 1μg/mL (r=0.9994), and high precision ( RSD<6.38%) .The lowest detected concentration was 0.012 mg/m3 , and the average recovery was 98.8%.Conclusions This is a simple method , easy to operate , not interfered by other ions , and can be used to detect the ammonia in the environmental air of laboratory animal facilities .

Objective To investigate the clinical effects of phacoemulsification with foldable posterior cham-ber intraocular lens implantation in the treatment of primary angle closure glaucoma with cataract .Methods 82 patients(82 eyes) with primary angle closure glaucoma with cataract were selected as the research objects ,and taken the treatment of combination of phacoemulsification and posterior chamber foldable intraocular lens implanta -tion.c The vision,intraocular pressure ,anterior chamber depth and angle opening changes before and after 6 months of operation were ompared ,and the postoperative complications were observed .Results 54 eyes achieved best corrected visual acuity of more than 0.5,12 eyes achieved best corrected visual acuity of 0.4,6 eyes achieved best corrected visual acuity among 0.1 to 0.3 and 6 eyes achieved best corrected visual acuity of less than 0.1.The difference between postoperative best corrected visual acuity and that before operation had significant statistical differences (χ2 =43.244,P<0.01).After having the operation ,the intraocular pressure of acute closed angle glaucoma pre clinic , onset period and chronic angle closure glaucoma had significantly improved compared to pre -operation ( t=7.955, 4.741,5.638,all P<0.01).The chamber depth of the three groups had also improved substantially compared to that of pre-operation(t=13.080,11.890,16.124,all P<0.01).The degree of angle opening of acute closed angle glau-coma and chronic angle closure glaucoma were better than that of pre-operation,and postoperatively ,the degree of an-gle opening of the former turned out to be wider than that of the latter (χ2 =9.246,P<0.05).There were no postop-erative intraocular lens clamping,corneal decomposition,severe complication of retinal detachment and macular cys-toids edema .Conclusion The phacoemulsification combined with intraocular lens implantation for the treatment of primary angle closure glaucoma with cataract can effectively improve visual acuity ,control intraocular pressure ,deepen the anterior chamber ,open angle ,and the adverse reactions occurred less ,which is worthy of clinical application .

Objective To explore the clinical effects of phacoemulsification with intraocular lens implantation for cataract patients who have had glaucoma and have undergone trabeculectomy before.Methods Phacoemulsification with intraocular lens implantation was performed on 33 patients(38 eyes)with cataract after trabeculectomy.The postoperative visual acuity,intraocular pressure(IOP),filtering bleb,corneal endothelium and complications were observed.Results No posterior capsular rupture occurred and all the lenses were inserted into the capsular bags.Follow up for 3 months at least.The correct visual acuities were≥4.7 in 30 cases(78.9%),≥5.0 in 6 cases(15.8%).Postoperatively,the IOP of all eyes was below 20mmHg and there was no change in the filtering bleb.The loss rate of the corneal endothelial cells was 15.07%after the operation.The density of the postoperative corneal endothelial cells was statistically significant different from those of the preoperative(t=4.582,P＜0.01).The complications occurred more than that of regular phaeoemulsification.Conclusions Phacoemulsification is convenient and effective in patients with cataract after trabeculectomy.The incision should be made without damaging the filtering bleb.The surgery requires skillfully and actively enlarging the small pupils and protecting the corneal endothelium.

The application of genetic engineering technology in modern agriculture shows its outstanding role in dealing with food shortage. Traditional medicinal plant cultivation and collection have also faced with challenges, such as lack of resources, deterioration of environment, germplasm of recession and a series of problems. Genetic engineering can be used to improve the disease resistance, insect resistance, herbicides resistant ability of medicinal plant, also can improve the medicinal plant yield and increase the content of active substances in medicinal plants. Thus, the potent biotechnology can play an important role in protection and large area planting of medicinal plants. In the development of medicinal plant genetic engineering, the safety of transgenic medicinal plants should also be paid attention to. A set of scientific safety evaluation and judgment standard which is suitable for transgenic medicinal plants should be established based on the recognition of the particularity of medicinal plants.
Genetic Engineering ; Plant Diseases ; genetics ; prevention & control ; Plants, Genetically Modified ; chemistry ; genetics ; growth & development ; metabolism ; Plants, Medicinal ; chemistry ; genetics ; growth & development ; metabolism

Adult ; Humans ; Male ; Malondialdehyde ; blood ; Submarine Medicine ; Superoxide Dismutase ; blood ; Young Adult

Animals ; Diazepam ; pharmacology ; Drug Antagonism ; Electroencephalography ; Female ; Male ; Pyrazoles ; poisoning ; toxicity ; Rats ; Rats, Sprague-Dawley

Aconitum ; poisoning ; Animals ; Disease Models, Animal ; Female ; Hemoperfusion ; methods ; Male ; Plant Poisoning ; therapy ; Rabbits

[Objective]To discuss the common complications and the methods of prevention and treatment in the operation of displaced acetabular fracture.[Method]Sixty-six cases with displaced aceuabular fracture were treated operatively from August 2005 to June 2009.Approach included anterior ilioinguinal approach,posterior Kocher-Langenbeck approach,or combined ilioinguinal and Kocher-Langenbeck approach.The functional results and complications were analyzed.[Result]All cases were followed up for an average of 29 months(12-46 months).According to the Matta evaluation,the anatomical reduction of acetabular fracture were in 34 cases,perfect in 25,and unsatisfactory in 7.According to the modified Merle d' Aubigne and Postel clinical evaluation standard,the clinical outcome was graded as being excellent in 23 cases,good in 29,fair in 8,poor in 6.Sciatic nerve injury after operation were noted in 2 hips,osteoarthritis in 14,heterotopic ossification in 10,and avascular necrosis of femoral head in 2.No infection and death were founded in this group.[Conclusion]The important factors to decrease the operative complications of acetabular fracture include correct fracture classification,suitable operative time,good operative approach,proficient technique of reduction and fixation and reasonable perioperative measures.

Objective To observe the effect and machanism of Xiaogu Granule (Granules for treating tympanites) plus ultrafiltration and reinfusion of ascites on refractory ascites resulted from hepatocirrhosis. Methods The 100 cases of refractory ascites due to hepatocirrhosis were randomized into two groups,with 50 cases in each. Both groups were administered basic treatment + ultrafiltration and reinfusion of ascites. The experimented group was added Xiaogu Granule and the control groups was added placebo. Before treatment, 3 and 6 months after treatment, the portal vein diameter (PVD), splenic vein diameter (SVD), blood flow quantity of portal vein (PVQ) and of splenic vein (SVQ), and the level of serum alanine aminotransferase, total bilirubin, endotoxin, nitrous oxide (NO) and endothelin (ET) of the two groups were measured respectively. Results After treatment,all the indices of two groups showed that the effect of experimented group was obviously better than that of control group, the difference was significant (P

Objective To compare the feasibility,effectivity and security of modified trabeculectomy in advanced stage angle-close glaucoma with cataract.Methods One hundred and forty-five patients (151 eyes) of advanced stage angle-close glaucoma with cataract were enrolled in this study.Among them,85 patients(91 eyes,observation group) were treated with modified trabeculectomy with the resection of deep scleral resection and 60 patients (60 eyes,control group) were treated with routine trabeculectomy.The visual acuity,intraocular pressure (IOP),type of filtering bleb and postoperative complication were compared.Results There was significant difference in the visual acuity ＜ 0.1,0.4-0.5,＞ 0.5 between after operation for 1 month and before operation in two groups (P ＜ 0.05).but there was no significant difference in the visual acuity 0.1-0.3 (P ＞ 0.05).The IOP before operation in observation group was (29.6 ± 4.1) mmHg (1 mmHg =0.133 kPa),12 months after operation was (13.1 ± 4.9) mmHg,the degree of decrease was (16.6 ± 5.0) mmHg.The IOP before operation in control group was (30.4 ± 6.3) mmHg,12 months after operation was (18.9 ± 3.0) mmHg,the degree of decrease was (10.4 ± 4.0) mmHg.The IOP after operation and the degree of decrease between two groups had significant difference (P ＜ 0.05).The functional filtering bleb(Ⅰ type and Ⅱ type) in observation group was 77 eyes(84.6％,77/91),and 51 eyes(85.0％,51/60) in control group,there was no significant difference (P ＞ 0.05).After operation for 1 month,there was 8 eyes appeared transient hypotony in observation group and 5 eyes in control group,there was no significant difference (P ＞ 0.05).Conclusion The modified trabeculectomy in advanced stage angle-close glaucoma with cataract can reduce IOP more effectively and not increase the intraoperative risk and postoperative complication.