Objective:To analyze the changes of chemical compositions and relative contents of volatile constituents in raw Xanthi-um sibiricum Patr. and fried Xanthium sibiricum Patr. Methods: Headspace solid phase micro extraction ( HS-SPME) combined with gas chromatography-mass spectrometry ( GC-MS) was used to study the volatile components in raw and fried products of Xanthium sibir-icum Patr. . A peak area normalization method was applied to calculate the relative percentage content of each composition. Results:Totally 26 and 27 kinds of compounds were isolated and identified respectively from raw and fried xanthium including 19 species and re-spectively accounting for 68. 69% and 90. 08% of the total volatile components. In the raw products, the components with high con-tents were camphor (15. 3%), eucalyptus oil alcohol (9. 6%) and borneol (6. 89%), while in the fried products, those were men-thol (24. 09%), borneol (18. 48%) and eucalyptol (7. 19%). Conclusion:The relative contents of components with effects on rhi-nitis in the fried products are obviously higher than those in the raw products. HS-SPME-GC-MS can be used to detect the volatile com-positions and their relative contents in the raw products and fried products of Xanthium sibiricum Patr. . From the changes of volatile components, theoretical basis can be laid for the components with certain efficacy in raw and fried Xanthium sibiricum Patr. , and scien-tific basis can be provided for their further study and comprehensive utilization.

Hospitals frequently end up losing medical lawsuits due to the following factors: inappropriate appointment of legal representatives, misconception and wrong application of the standard of proof required for lawsuits, ignorance of the time limit for submitting lawsuit documents, and lack of communication with the judges on the proof value of medical literature. In order to guarantee their legitimate rights and interests, hospitals ought to appoint health lawyers and medical experts as their legal representatives, have a good grasp of the standard of proof of reasonable probability, adhere to the time regulations in lawsuits, strengthen communication with the judges with regard to medicine, and understand the legal rules of arbitration. Only by doing so can their legal rights and interests avoid being encroached upon.

Objective To explore the influence of late pregnancy complicating uterus myoma on the maternal and fetal out‐comes .Methods A total of 368 patients with late pregnancy complicating uterus myoma were taken as the observation subjects . The influence situation of size ,number and location of uterus myoma on the pregnancy ,delivery ,puerperium and maternal‐fatal out‐comes was analyzed .Results The incidence rate of threatened abortion ,threatened premature delivery ,abnormal fetal position ,pla‐centa praevia and placental abruption in complicating uterus myoma with diameter≥5 cm ,multiple ,intramural and cervial myoma was obviously higher than that in complicating uterus myoma with diameter<5 cm ,single and subserous myoma or submucous my‐oma (P<0 .05) .The cesarean section rate for the uterus myoma with diameter≥5 cm ,multiple and intramural myoma was signifi‐cantly increased (P<0 .05) .The incidence rate of fetal distress ,fetal anomaly ,premature infant ,low birth weight infant ,and neo‐natal asphyxia in uterus myoma with diameter≥5 cm ,multiple ,and intramural myoma was obviously increased(P<0 .05) .Conclu‐sion The late pregnancy complicating large ,multiple and intramural myoma has more obvious adverse reactions on maternal‐fetal prognosis .

Objective To compare the effects of transcranial and trans-spinal low frequency magnetic stimulation (MS) on the expression of growth-associated protein-43 (GAP-43) in the spinal neurons of rats after spinal cord injury (SCI). Methods Forty-five Sprague-Dawley female rats were divided randomly into a transcranial magnetic stimulation (TMS) group,a trans-spinal cord magnetic stimulation group and a model control group with 15 in each group.Another 6 rats served as a normal control group.Spinal cord injury at the T9-10 level was modeled according to the modified Allen's method.The rats in the two MS groups received magnetic stimulation at 0.5 Hz and 75％ of the maximum output intensity ( 1.9 T) of the magnetic stimulator beginning 24 h post injury.They received 30 pulses daily for 28 days while the rats in the model control group were not exposed to MS.Basso-Beattie-Bresnahan (BBB) scale scores and motion evoked potentials (MEPs) were measured at the 1st,7th,14th,21 st and 28th day post injury.The expression of GAP-43 protein was studied using immunohistochemical methods and semi-quantified by Western blotting. Results At the 4th week post injury the average BBB scores in the model control group,the TMS group and the trans-spinal cord magnetic stimulation group were all significantly different. MEP amplitudes decreased significantly in all groups after the injury,and the latency was prolonged,but there were no significant differences among groups at any time point.GAP-43 expression also increased after SCI,and it was significantly higher in the MS groups than in the model control group at 4 weeks. Moreover,the expression of GAP-43 in the trans-spinal cord MS group was significantly greater than in the TMS group.Conclusion Magnetic stimulation can promote nerve regeneration and improve locomotor recovery after spinal cord injury,at least in rats.The effect of trans-spinal cord magnetic stimulation is superior to that of TMS.The mechanism may be related to the activation of central pattern generators.

Objective To analyze the clinic and imaging characteristics as well as development of intervertebral disc calcification in children.Methods 6 cases underwent intervertebral disc calcification.X-ray films and CT scan of cervical spine had been taken in all cases,two cases had MRI examination.All cases were followed-up for 2 to 3 years by radiograph and CT.Results Only one intervertebral disc involved in all cases.X-ray and CT findings included disc calcification,disc herniation and change of vertebral boy in form.The clinical symptoms disappeared after conservative therapy 2 to 4 weeks.But the disc calcification disappeared until 6 months to 3 years.Conclusion Intervertebral disc calcification in children is a self-limiting disease with excellent prognosis,its diagnosis depends on imageology.

Based on the research project of the French language teaching reform in Kunming Medical College——the Application and Analogy of Modern Foreign Languages Pedagogy,the successes and disadvantages of the visual-audio-oral course were studied.The teaching reform's purpose and focal points were primarily introduced.Then,the successful experiences of academic period arrangement,teaching materials selection,in-class instruction and students' academic achievements were elaborated.Finally,by making a comparison of students' French language proficiency between the present experimental classes and the former ordinary classes,a few disadvantages of the reform were interpreted.

On the clinic,the main treatment of autoimmune diseases were used immunosuppressant,but large-scale use of immunosuppressive agents could cause of the delayed dysfunction,side effects such as infection,tumors and so on,so-induced immune suppression and immune tolerance response was the most effective way.So far,dendritic cells(DC) was the most powerful professional antigen-presenting cells.On one hand,DC as a full-time antigen-presenting cells and trigger regulation of the inherent and acquired immune response,start the immune response;On the other hand,DC through a variety of mechanisms induced antigen-specific T cells incompetent,show strong plasticity in immune tolerance and play an important role in immune regulation.Nuclear factor(NF)-?B is a nuclear transcription factor.In the immune response,NF-?B in the dendritic cells regulation of their own including development,maturity,the antigen-presenting,to stimulate T cells functions and so on.Such adjustments play an important role in the incidence of autommine diseases,could became a new target treatment of autommine diseases.

0.998 8).The minimum detection limit were 0.1～0.4 mg?L-1.The average recovery rates were no less than 96%(RSD≤7.19%).There were significant difference among the concentration of Ara-C and Ara-U in plasma,cerebrospinal fluid and testicular tissue.Ara-C in plasma hadn't been determined in low-dose group and medium-dose group except for Ara-U.CONCLUSIONS: The method is simple,rapid,accurate and reproducible for plasma concentration monitoring and pharmacokinetic study of Ara-C and Ara-U.

Objective To study effects of Kanglaite injection combined with gefitinib on inflammatory factors and immune function in patients with advanced lung cancer.Methods 98 patients with advanced lung cancer in First People 's Hospital of Yuhang District from December 2013 to September 2016 were selected and randomly divided into gefitinib-treated group and combination drug-treated group with 49 cases in each group.The patients in the gefitinib-treated group were treated with gefitinib, the patients in the combination drug-treated group were treated with gefitinib and Kanglaite injection.The clinical efficacy, inflammatory factors, immune function, quality of life and adverse reactions were observed and compared between the two groups. Results The efficacy and control rate in combination drug-treated group were higher than gefitinib-treated group(P<0.05).The inflammatory factors such as CRP, IL-6 and TNF-αwere significantly improved in the two groups before treatment, and the combination drug-treated group improved significantly better than gefitinib-treated group(P<0.05).The levels of cellular immune factors in the two groups were significantly improved compared with that before treatment, and the combination drug-treated group was significantly better than the gefitinib-treated group(P<0.05).The quality of life in the combination drug-treated group was significantly higher than the gefitinib-treated group(P<0.05).The incidence of adverse reactions such as nausea and vomiting, thrombocytopenia, and renal dysfunction in combination drug-treated group was lower than in the gefitinib-treated group( P<0.05).Conclusion Application of gefitinib combined with Kanglaite injection in the treatment of patients with advanced lung cancer treatment is more significant, can effectively reduce the systemic inflammation and adverse reactions, improve immune function and quality of life.

AIM: To evaluate Ribbond ultrahigh molecular weight polyethylene fiber (UMWPF) on hamster's pouch by oral membrane irritation. METHODS: Experiments were performed at the Animal Experimental Center of Henan Province from July to December 2004. Ten healthy male golden hamsters aged 60-70 days were selected. The samples of UMWPF, positive and negative control group (n=10, diameter 4 mm, thickness 1 mm) were fixed on the oral membrane of hamster's pouch with medical thread. The thread should pass through membrane and fixed on the animals' skin. The experimental samples were on the one side of pouch membrane (UMWPF toward membrane); the positive and negative samples were on the cephalic and tail of the other side respectively. After two weeks' systemic and local observation, the local oral membrane specimens were viewed under microscope. RESULTS: Totally 10 hamsters were included in the final analysis. ①2 weeks after the surgery, the animals pouch membrane contacting with the experiment and negative samples showed no hyperemia, hydrops, erosion or ulcer, and where positive samples contacted showed rough; 7 of them showed light hyperemia and hydrops. ②The epithelia layer of the animal membrane contacting with experiment and negative samples were complete, which cells were in order, epithelial processes stretched to the basal layer. There was no inflammatory cells infiltration, congestion or edema in the connective tissue, but which showed contrast in the positive group. ③The epithelial cells of the experiment and negative groups had no changes in morphology, while in the positive group a few cells swelled and their nucleolus were more clear than normal, and their shapes were circle which was different from normal. There were no systemic or local abnormal response to UMWPF and that the histopathological findings were also normal. CONCLUSION: Ribbond ultrahigh molecular weight polyethylene fiber has no abnormal irritating response to oral membrane of golden hamster.