The same paraffin-embedded specimen of skin biopsy from each of 29 patients with cutaneous malignant lymphomas (CML) and 7 patients with cutaneous lymphoid infiltrates (CLI) was estimated simultaneously and retrospectively by karyomorphometry and proliferating rate assay. The results showed that the differences in nuclear volumes (NVs), count of argyrophilic nucleolar organizer regions(AgNORs) and proliferating cell nuclear antigen (PCNA-PC10) indices (PC10Is) between CML and CLI were statistically very significant. The NVs and PC10Is were correlated to the death rate of patients. NVs were also correlated to AgNORs.

Objective To study the values of quantitative parameters of DCE-MRI in diagnosis of benign and malignant liver tumors and to explore its diagnostic efficacy.Methods Consecutive 25 patients with benign or malignant liver tumors underwent plain scanning and dynamic contrast-enhanced MRI,and total 28 lesions were confirmed by pathology or follow-up.The DCE-MRI quantitative parameters (Ktrans ,Kep ,Ve and iAUC)of the lesions and the surrounding normal liver tissue were acquired.The inde-pendent sample t test was used to compare the quantitative parameters between different tumors.The optimal parameters values of benign and malignant liver tumors were determined by plotting ROC curves.Results The mean quantitative parameters of HCC and benign lesions were larger than those of the surrounding normal liver tissue.Ktrans ,Kep and iAUC between HCC and surrounding nor-mal liver tissue had significant differences (P <0.05),Ktrans and iAUC between benign lesions and surrounding normal liver tissue had a significant difference (P <0.05).Ktrans ,Kep and iAUC between HCC and benign lesions had significant differences (P <0.05). The diagnostic effectiveness of Ktrans = 0.21 5 min-1 ,with sensitivity of 81.3% and specificity of 66.7%,was greater than that of Kep =0.477 and iAUC=24.706.Conclusion The DCE-MRI quantitative analysis can provide a reference for the differential diagnosis of HCC from benign liver tumors.

ObjectiveTo study the distribution, developmental patterns, and hormonal control of androgen re- ceptors(AR) in the developing and ethane dimethane sulfonate(EDS) treated male SD rat epididymis. MethodsThe ABC technique of immunohistochemistry and image analysis were used to assess optodensity means (OPTDM) of AR, providing a measure of relative nuclear AR concentration. ResultsThe specific AR immunostaining was observed in the nuclei of epididymal epithelium, peritubular smooth muscle cells and intertubular connective tissue cells. The rela- tive AR concentrations varied with the different segments of the epididymis in the adult rat(P<0. 05 or P<0.01). AR protein was highest in the caput (0. 763--0. 026),lowest in the corpus (0. 712±0. 025) and intermediate in the cauda (0. 736±0. 008). Levels of epididymal AR changed with development. In the cauda, AR level was highest on day 21 (0. 773±0. 028),intermediate on day 35(0. 762±0. 022),and lowest on day 90～120(0. 736±0. 008). The 90～120d group was significantly different from the 21d group (P＜0. 01)and 35d group (P＜0. 05). After the adult rats were treated with EDS to eradicate Leydig cells and endogenous testosterone, it was observed that the OPTDM of AR in the epididymal cauda epithelium was significantly reduced (P＜0. 001), and was restored to the control level by using ex- ogenous testosterone replacement (P＜0. 001). Conclusion These results suggest that the epididymal corpus depends least on androgens and the AR expression in the epididymis decreases with age and is dependent on circulating andro- gens.

Objective: To evaluate the therapeutic effect of gamma knife on patients with intracranial cavernous angioma (CA). Methods: The medical records of 122 patients (134 lesions) who underwent radiosurgery were reviewed retrospectively. Results: hT e average follow-up period was 43 months. No patient died. One patient underwent CA resection. In patients with epilepsy, 83% patients showed alleviation of seizures. About 44% of the lesions shrank in size after treatment with gamma knife radiosurgery (59/134). Seven cases had hemorrhage again after radiosurgery, and the overall annual hemorrhage rate was 1.6%. Edema was found in 11.5% patients (14/122) and all patients showed improvement atf er treatment. Conclusion: Gamma knife is a safe treatment for CA, which could obviously improve the symptoms of epilepsy. Gamma knife radiosurgery is the if rst option for the treatment of cavernous sinus angiomas.

Acute Disease ; Adult ; Female ; Humans ; Male ; Middle Aged ; Occupational Diseases ; chemically induced ; Trimethyltin Compounds ; poisoning

Objective To investigate the effect of genetic alteration(homozygous deletion and point mutation)and expression of p16 gene and p16 protein on hilar cholangiocarcinoma(HCGC) . Methods Genetic alteration and expression of p16 protein were detected by polymerase chain reaction single strand comformation polymorphism (PCR-SSCP) and immunohistochemical method in 36 HCGC tissues. Results p16 gene revealed alteration in 21 of 36 HCGC tissues (58.3%),among which 8 had homozygous deletion and 13 had point mutation. In HCGC tissues, 8 revealed no p16 protein expression and 10 showed low level expression of p16 protein. Conclusions The alteration of p16 gene and abnormal expression of p16 protein are significantly correlated with the biological behavior and clinical staging of HCGC,and may be helpful to evalute the malignant degree of HCGC and the patients prognosis.

Objective To investigate the biological effects of bcl-2 gene on neurons. Methods The recombinant expression plasmid pc DNA3-bcl-2 was constructed from pSFFV-bcl-2,then it was introduced into PC12 cell line by liposome method.Western blotting and immunohistochemistry in situ were applied to exam the exogenous gene expression.The two groups of cells(Group A,PC12 transfected by pcDNA3-bcl-2 and Group B,PC12 transfected by pcDNA3) were exposed to cisplatin with the concentration of 10*!?mol/L,50*!?mol/L, and 100*!?mol/L 72 hours later,the survival cells were estimated.Cell cycle indexes between these two groups were also studied by FCM. Results The recombinant expression plasmid pcDNA 3-bcl-2 was constructed successfully and PC12 cell line transfected by the plasmid could express Bcl-2 protein effectively.Compared with the control(25 79%),there was a significant decrease of cells from the S phase in PC12 with bcl-2 gene(8.81%).After exposed with 10*!?mol/L,50*!?mol/L,and 100*!?mol/L cisplatin,the surviving cells in group A were 276?13,185?11 and 108?10 respectively,which increased much more than in group B while they were 100?9,12?3 and 2?2 accordingly.Conclusions bcl-2 can protect PC12 cells against cytotoxic insults of cisplatin,and it suggested that it might act via cell cycle controlling.

The partial segment of Marek′s disease virus (MDV) glycoprotein B (gB) gene was amplified by PCR. The segment was cloned into pET-28a vector to obtain the recombinant pET-gB plasmid. The recombinant plasmid was transformed into E.coli BL21,and expressed in very high level as inclusion body after induced with 1.0mmol/L IPTG. The inclusion body was solubilized in urea (8mol/L) . The purified protein was obtained by use of His?Bind affinity chromatography. Mice were immunized i.p. by the purified protein to make the polyclonal antibody. The titer of the antibody by indirect ELISA was 1?10~ -5 . Moreover, the analysis by western blot proved that antibody was specific to the recombinant protein. These works lay a favorable foundation for the study of the immune response by MDV gB.

Objective To research the social economic development and service needs of families with more than one disabled in Henan, China. Methods The data of 2016 Basic Service Status and Needs of People with Disabilities in Henan was analyzed. Results A to-tal of 74,732 (4.0%) people with disabilities from all 19 cities in Henan had been surveyed, 54.9%were males and 83.2%were from rural ar-eas. In terms of social and economic development, 41.8%of people with disabilities in urban areas were below least living baseline, 14.7%met standards of low income;23.9%of people with disabilities in rural areas were recorded as national poverty household, 35.7%were not recorded as national poverty household, 39.9%were not in poverty. In terms of housing, 75.3%of people with disabilities in urban areas had their own houses, 7.1%had housing security welfare, 12.9%had no house; 70.5%of people with disabilities in rural areas had their own houses in good condition, 3.5%had their own houses which were identified as houses in risk, 15.9%had their own houses which were sus-pected to be houses in risk, 1.7%had no house, and 2.2%had their own houses reconstruction. There were significant differences in the eco-nomic and housing conditions among different types of disabilities (χ2>51.267, P<0.001), and in people under standards of low income and others among different levels of disabilities (χ2>8.400, P<0.05). For people with disabilities in rural areas, there were significant differences in the economic and housing conditions among different types of disabilities (χ2>70.149, P<0.001), and no significant difference was found among different levels of disabilities (χ2<6.446, P>0.05). In terms of employment needs, vocational skills training 5.0%, introducing a job 3.9%, practical training in rural areas 6.6%, capital credit support 7.6%, and others 10.7%. There were significant differences in the employ-ment needs among different types of disabilities (χ2>73.180, P<0.001), and in the needs of vocational skills training, introducing a job, practi-cal training in rural areas and capital credit support among different levels of disabilities (χ2>11.440, P<0.01). In terms of caring service needs, home caring 54.1%, day care 11.1%, and institutional residential care 2.9%. There were significant differences in caring service needs both among different types and levels of disabilities (χ2>38.960, P<0.001). In terms of rehabilitation needs, surgery 2.1%, medicine 22.5%, functioning training 16.1%, assistive devices 29.8%, and nursing 14.8%. There were significant differences in rehabilitation needs among different types of disabilities (χ2>99.220, P<0.001), and in needs of nursing among different levels of disabilities (χ2=12.465, P<0.01). In terms of barrier-free reconstruction, sloping and handrail 17.7%, door 14.2%, bathroom 30.3%, kitchen 20.5%, visual doorbell with flash 3.4%, gas leak alarm device 2.3%, and internet access and screen software 0.6%. There were significant differences in barrier-free recon-struction among different types of disabilities (χ2>68.000, P<0.001) except visual doorbell with flash, gas leak alarm device and internet ac-cess and screen software. There was significant difference in needs of flash and visual doorbell, gas leak alarm device and internet access screen software among different levels of disabilities (χ2>46.960, P<0.001). Conclusion The social economic development of families with more than one disabled were faced risk. Recommendation to policy development included to improve the support efforts, and ensure their ba-sic housing, pay attention to their individual differences in employment poverty alleviation needs and provide more opportunities to get jobs, and provide financial support, increase support for home care, increase inputs of funding and assistive devices for rehabilitation services, and attach importance to their needs of barrier-free reconstruction.

Background Acute bacterial conjunctivitis is a common ocular infectious disease.It can be cured by topical administration of antibiotics,but these antibiotic eye drops often was used several times per day at present.Azithromycin is thought to offer less using times and shorten the duration of treatment.Objective The purpose of this clinical trail was to evaluate the clinical efficacy and safety of topical administration of 1％azithromycin eye drops for the treatment of acute bacterial conjunctivitis.Methods This study was approved by Ethic Committee of Beijing Tongren Hospital and followed the Declaration of Helsinki.Written informed consent was obtained prior to entering into this trial.A randomized,double-blind and placebo-controlled study was designed.One hundred and eighty patients with acute bacterial conjunctivitis were enrolled in Affiliated Hospital of Qingdao Medical College and Qingdao Municipal Hospital from may,2011 to September,2011.Azithromycin eye drops at the concentration of 1％ was topically administered on 89 eyes of 89 patients twice per day initial and once per day later for 9 days in the trial group,and placebo eye drops was used on 91 eyes of 91 patients in the same way in the control group.The inflammation response of all eyes was examined and scored under the slit lamp microscope,and germiculture was carried out.All adverse responses were assessed during the follow-up.Clinical efficacy was evaluated with the clinical cure rate as the primary indicator and bacterial clearance rate as the secondary indicator.The safety of drug was determined,including the ocular irritation sign,lens transparency and intraocular pressure.The differences of the examining results mentioned-above were compared with Chi Square test.Results One hundred and eighty patients completed the clinical trials according to the program without lost and exclusion cases.The clinical cure rate in 89 subjects in the trial group and 91 control individuals was 76.40％ (68/89) and 43.96％ (40/91),respectively,with significant difference between them (x2=19.73,P＜0.01).The bacterial eradication rate was 85.71％ (24/28) in the trial group and 60.53％ (23/38) in the control group,showing a significant difference (x2 =4.99,P＜0.05).Both 1％ azithromycin eye drops and placebo were well-tolerated with a low incidence of adverse events.Conclusions One percent azithromycin eye drops is effective and safe for the treatment of acute bacterial conjunctivitis with less adverse reaction.