Acute kidney injury ( AKI) in newborns is one of neonatal critical disease,the incidence of AKI is 23%and the mortality is 25%~50%in neonatal intensive care unit (NICU).AKI in newborns is ab-normal renal function due to many various unfavorable factors,such as neonatal asphyxia,low blood volume, shock,hypoxia,hemolysis,low temperature,the clinical manifestations are oliguria or anuria,electrolyte disturb-ance,acid-base imbalance, high concentrations of metabolites in plasma and renal excretion ( urea, creatinine etc.) .At present,there are no effective prevention measures.In recent years,with the progress of blood purifica-tion technology,continuous blood purification ( CBP) has been used from AKI in children to AKI in newborns. Because of the neonatal hemodynamic instability and blood characteristics of small capacity, the application of CBP in the neonatal AKI still faces many problems.The paper summarizes high risk factors and diagnostic crite-ria of AKI in newborns,related problems of neonatal CBP treatment.

Objective To draw up the learning strategy scale for vocational nursing students and discuss its characteristics. Methods Structure model of learning strategies was constructed by summarizing and analyzing current learning connotation and structure research, through the view of application combining with theory. Results The results showed that the learning strategy of the vocational nursing students included five factors: cognitive strategy, meta-cognitive strategy, emotions regulation strategy, resource management strategy and practical operation strategy. Conclusions The strategy scale is very reliable and valid and can be used to measure these learning strategies. Results also indicates that excellent students show much more profitable strategies than students of lower levels, no difference is found in different grades.

This research explored the rehabilitation of hearing and speech for children with hearing and speech impairments using the theory and method of International Classification of Functioning, Disability and Health. An evaluation system of rehabilitation for children with hearing and speech impairment, which consisted of the functions and structures of cognition and speech, verbal behaviors, activity and participation, and environments, had been developed.

Objective To understand the current status of learning motivation of high vocational nursing students, In order to increase their learning effect and teach quality. Methods 334 high vocational nursing students were investigated with college students learning motivation questionnaire, the results underwent analysis. Results High vocational nursing students had low learning motivation, their learning motivation was diversified. Significant difference existed between top students and low students. Conclusions High vocational nursing students had weak learning motivation, and is not related with grades and source places. It had individual orientation and utility color, but low social property. Learning motivation is an important factors for academic achievement.

Objective To construct the recombinant of hepatocellular carcinoma-targeting adenovirus containing r-Caspase-3 gene and provide the gene therapic strategy for hepatocellular carcinoma. Methods The pAdTrack-EAFP-PALB was constructed and the r-Caspase-3 gene was subcloned into the vector. The linearized shuttle plasmid was homogenously recombined with AdEasy-1 in BJ5183 cells. The candidate clone was analyzed by restriction endonuclease digestion and sequencing, and then pAdEasy-EAFP-PALB/r-Caspase-3 vector was digested with PacⅠand transfected into AD293 cells for packaging and amplifying, recombinant virus was constructed successfully. Infection titer and efficiency of recombinant virus were monitored by green fluorescent protein (GFP) expression. The expression of r-Caspase-3 in infected HepG2 cells was detected by RT-PCR and Western blot. The apoptosis of HepG2 cells was detected by SRB dyeing method. Results Shuttle vector pAdTrack-EAFP-PALB/r-Caspase-3 was correct after identification by restriction endonuclease analysis and sequencing. By PCR and PacⅠ restriction endonuclease analysis, the homologous recombinant of pAdEasy-EAFP-PALB/r-Caspase-3 was successful. The expression of GFP was observed when linearized pAdEasy-EAFP-PALB/r-Caspase-3 was transfected into AD293 cells. AD293 cells could be infected repeatedly by recombinant adenovirus. The expression of r-Caspase-3 gene on HepG2 cells was detected by RT-PCR and Western-blot methods respectively, which confirmed that the Ad-EAFP-PALB/r-Caspase-3 was constructed successfully. The specificity of Ad-EAFP-PALB/r-caspase-3 which targeting induced hepatocellular carcinoma cells was founded by SRB dyeing test. Conclusion The Recombinant of hepatocellular carcinoma-targeting adenovirus containing r-Caspase-3 gene was constructed successfully and which established the foundation of r-Caspase-3 gene therapy in future research to hepatocellular carcinoma.

Obej ctive To inev stigate the situation of hands only cardiopulmonary resuscitation ( CPR) and training effectiveness of CPR in middle school students.Methods By the principle of random stratified cluster sampling,7 687 middle school students in Changsha city were trained on hands only CPR through the scene theoyr teaching and anthropom orphic dummy in this study.The training content icn lued d who was rescued by CPR, whne pait ne ts require CPR,h ow logn cna causei rreversible brain damage, the stna dardized process fo CPR,the position of CP R,the depth of CPR,and the ratios of breath-to-compression. We analyzed the middle school students′awareness of hands only CPR know ledge before and after the train-ing,investigated the middle school studne ts′willign ness and way to accept knowledge of first aid.Re sulst All of 7 687 middle scoh ol students inC hangsha cti y received questionnaire us rvey and trained on hands only CPR,including 3 778(49.15%) boys and 3 909 girls(50.48%).The knowledge of hands only CPR was mainly derived from the book,television,network,and education from schools.Before training,the awareness rates of the knowledge on who was rescued by CPR and the position of CPR were more than 50%,and the awareness rates on the rest of know ledge were less than 50%.The awareness rates on hands only CPR were more than 75% by training, which were significantly increased compared with tho se before training ( P <0.01).Conclusion The knowledge on hands only CPR is very scarce in middle school students in Changsha city,and the awareness on hands only CPR can be significantly improved by the scene theory teaching and an-thropomorphic dummy.

Recent studies indicated that epigenetic abnormality is the important molecular mechanism in leukemia. The change of epigenetic modification occurs in most kinds of leukaemia. Based on the epigenetic modification, the therapies of leukemia with hypomethylating agents and histone deaeetylases inhibitors, which are different from traditional chemotherapy are applied in the treatment of leukemia at beginning. The aim of this article is to summarize the recent advances of DNA methylation and histone modification in leukaemia occurrence, treatment and prognosis.

AIM: To observe the efficacy of ketosteril in treatm en t of chronic renal failure with high level of plasma Cys-C. METHODS: 60 patients with chronic renal failure were randomly divided into treatmen t group and control group. The control group was adopted a low protein and low p hosphorum diet and correcting symptoms; the treatment group, based on the therap y of the control group, was given ketosteril; 30 healthy subjects were used as t he normal control to observe changes of Cys-C, Scr, TC, HB, and HDL before and after the treatment. RESULTS: The levels of Cys-C and Scr decre ased after the administration of ketosteril (P

Child, Preschool ; Dexamethasone ; adverse effects ; Diaphragm ; drug effects ; Humans ; Infusions, Intravenous ; Male ; Spasm ; chemically induced

Animals ; Atherosclerosis ; blood ; drug therapy ; pathology ; Dehydroepiandrosterone ; blood ; physiology ; therapeutic use ; Dehydroepiandrosterone Sulfate ; blood ; therapeutic use ; Hormone Replacement Therapy ; Humans