[Objective]To detect the expression of tumor necrosis factor-?(TNF-?)and interleukin-18(IL-18)protein in herniated intervertebral disc,and discuss the role of TNF-? and IL-18 protein in the course of the intervertebral disc degeneration and to clarify the mechanism of the lumbar intervertebral disc degeneration and herniation. [Method]Forty nine specimens were studied from December 2005 to April 2007 in the First Affiliated Hospital of Shihezi Medical College.Experimental group contained thirty-four herniated intervertebral disc and control group contained fifteen discs,which were collected from the patients with spine fracture or autopsy.The specimens were divided into two parts,one part was fixed in 10% formalin about 24 hours and was embedded in paraffin.Four ?m serial sections and S-P method were used.The sections were stained with TNF-? and IL-18 monoclonal antibodies,and the results were observed by OLYMPUS microscope.Another part was changed into well-distributed liquid,which was determined by Elisa method respectively,in order to detect density of TNF-? and IL-18 protein.Software SPSS 13.0 was used for statistical analysis.[Result]1 TNF-? and IL-18 protein were expressed mostly in cytoplasma.The expression of IL-18 in the experiment group was obviously higher than in the control group.There were statistical significances(P

BACKGROUND:Deluxe-PS knee prosthesis is designed based on the anatomical characteristics of the Chinese peoples’ knee joint, especial y the morphological features of femoral condyle. The distance between median and lateral knee prosthesis femoral condyle is less than that of the imported prosthesis for 3.5 mm.
OBJECTIVE:To explore the short-term efficacy of Deluxe-ps knee prosthesis in simultaneous bilateral total knee arthroplasty.
METHODS:Fifteen knee osteoarthritis patients (30 knees) were included as the experimental group, and treated with simultaneous bilateral knee arthroplasty by Deluxe-ps knee prosthesis. Twenty patients (40 knees) in the control group were treated with simultaneous bilateral knee arthroplasty by PFCSigma knee prosthesis. The knee society score, hospital for special surgery knee score and knee joint range of motion were compared between two groups;the operation time and the intraoperative blood loss were compared between two groups.
RESULTS AND CONCLUSION:The patients in both groups were fol owed-up for 12 to 24 months, with an average of 16 months. Postoperative knee pain was relieved, and the joint function was recovered satisfactorily. The knee society score, hospital for special surgery knee score and knee joint range of motion in two groups were improved significantly after treatment (P<0.05). There were no significant differences in postoperative knee society score, hospital for special surgery knee score and knee joint range of motion between two groups (P>0.05), and there was no significant difference in intraoperative blood loss between two groups (P>0.05), while there was significant difference in operation time between two groups (P<0.05). The results showed that the short-term effect of Deluxe-ps knee prosthesis in simultaneous bilateral total knee arthroplasty was satisfied.