BACKGROUND:It has become a hotspot to prepare the bone repair material that exhibits natural bone structure and is used in combination with biological factors. OBJECTIVE:To prepare the recombinant human bone morphogenetic protein-2 (rhBMP-2)/bone repair material, and to evaluate its capacities of release, activity and ectopic osteoinduction. METHODS:A collagen-binding domain was added to the N-terminal of native rhBMP-2 that allowed bind to collagens in the bone repair material. Then, rhBMP-2/bone repair material was obtained through freeze-dried method. The releasing ability of rhBMP-2 in vitro was assayed by ELISA. C2C12 cell lines were loaded to the composite material with 0.25, 0.5 and 1 μg rhBMP-2, respectively. Afterwards, alkaline phosphatase activity was detected at 72 hours. The composite materials with 0, 2, 5 and 10 μg rhBMP-2 were implanted into the quadriceps of Sprague-Dawley rats, respectively. Alkaline phosphatase activity and the newly formed bone were detected at 2 and 4 weeks after implantation. The CY-7-labeled composite material was implanted into the quadriceps of Sprague-Dawley rats to observe its stability. RESULTS AND CONCLUSION:Substantial y no rhBMP-2 from the rhBMP-2/bone repair material was released within 45 days. The alkaline phosphatase activity of C2C12 was in a rise with the increased concentration of rhBMP-2. The stability of the composite material in vivo was better, the alkaline phosphatase activity and ectopic bone formation increased as the concentration of rhBMP-2 rose. To conclude, the rhBMP-2/bone repair material preserves the stability of rhBMP-2, and improves ectopic osteoinduction ability.

Background:Chronic atrophic gastritis( CAG)is a common type of gastritis and is closely related to the pathogenesis of gastric carcinoma,however,the efficacy of its drug treatment is not clear. Aims:To study the therapeutic effect of capsaicin on CAG in rats. Methods:CAG rat model was established by intragastric administration of sodium deoxycholate, ethanol and ammonium hydroxide. Forty-four survival model rats were randomly divided into CAG model group and low, moderate,high dose capsaicin groups,rats in the latter 3 groups were intragastrically administrated with 1 mg·kg-1 ·d-1 , 10 mg·kg-1 · d-1 ,30 mg · kg-1 · d-1 capsaicin,respectively,for 28 days. The inflammation and degree of atrophy of gastric mucosa were observed by HE staining. Gastric mucosal levels of pepsinogen( PG)Ⅰ,PGⅡ were determined by ELISA. The protein expressions of IKKβ,NF-κB,XIAP were determined by Western blotting. Results:The differences in inflammation and degree of atrophy of gastric mucosa,levels of PGⅠ,PGⅡ,protein expressions of IKKβ,NF-κB,XIAP were statistically significant among model group,low,moderate,high dose capsaicin groups( P <0. 05 ). With the increasing of capsaicin dose,inflammation and degree of atrophy of gastric mucosa were improved in a dose-dependent manner(P<0. 05),protein expressions of IKKβ,NF-κB,XIAP were decreased. Conclusions:Capsaicin is effective for the treatment of CAG.

OBJECTIVE: To determine the expression of palate, lung, and nasal epithelial clone (PLUNC) in na sal polyps (NP) and evaluate its association with clinical severity. METHOD: Twenty-eight NP patients (primary polyp, 15; recurrent polyp, 13) and 16 normal controls (healthy uncinate process) were enrolled, the expression of PLUNC was examined in nasal tissues by immunohistochemistric staining, quantitative PCR and ELISA respectively. The protein level of PLUNC in nasal polyps was correlated with nasal symptom score (nasal congestion and rhinorrhea, respectively). RESULT: PLUNC was mainly distributed in the epithelial layer and submucosal glands in nasal tissues. The staining intensity and mRNA level of PLUNC were significantly decreased in polyp tissues than in normal controls (P < 0.01). The protein levels of PLUNC were 0.33 +/- 0.11 and 0.15 +/- 0.05 in primary and recurrent polyp tissues (P < 0.01), and were 0.32 +/- 0.14 and 0.19 +/- 0.07 in small-size and big-size polyp tissues (P < 0.05). The protein level of PLUNC in polyp tissues significantly correlated with both nasal congestion score and rhinorrhea score (r = -0.51 and r = -0.57, P < 0. 01). CONCLUSION Decreased PLUNC in polyp tissues indicated that impaired innate immunity may account for the pathogenic process of NP. Thus upregulating PLUNC may represent a promising therapeutic target for the management of NP.
Adult ; Case-Control Studies ; Female ; Glycoproteins ; metabolism ; Humans ; Male ; Middle Aged ; Nasal Polyps ; metabolism ; pathology ; surgery ; Phosphoproteins ; metabolism ; Recurrence ; Young Adult

Objective To emphasize the correlation and concordance of electronic medical record through analysis of frequently asked problems on handwritten medical record.Methods The problems of electronic medical record were classified and found the typical cases to analyze.Results In order to improve the quality of medical record,the quality control of electronic medical record must be strengthened and the phenomenon that some doctors pay attention to hand-written med-ical record more than electronic medical record must be overcame.Conclusion The normative management of electronics medical is strengthened that is fundamental guarantee for paper-based medical records.

Objective To detect the physico-chemical properties , cytotoxicity and antimicrobial activity of dressings containing Ag +.Methods The morphology of this dressing was shown by the scanning electron microscope ( SEM ) .The cytotoxicity was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) method.Using the shake flask test method , the antibacterial effect of dressings was studied .Result There was no significant difference in the water vapor transmission rate between the dressings (P>0.05).Compared with the alginate calcium dressing ,silver ion dressings had a stronger swelling rate .A higher ion concentration would lead to a much larger swelling rate and slower degradation . A lower cytotoxicity was exhibited among the dressings .The silver ion dressing had stronger bacteriostasis to Gram-positive ( G+) and Gram-negative ( G-) bacteria than the alginate calcium dressing .Conclusion The experiment has proved that the silver ion dressing has stronger antibacterial activity and lower cytotoxicity , and it is more effective for wound surface healing, with a shortened treatment course .

Background:Colorectal polyps are common gastrointestinal diseases,which are closely related to the development of colorectal cancer. Aims:To investigate the clinical features of high-risk people with intestinal polyps in colorectal cancer screening. Methods:People in Hongkou District,Shanghai,who were positive in fecal occult blood test or of the high risk category in colorectal cancer questionnaire from April 2013 to December 2014 were enrolled,and colonoscopy was performed at Branch of Shanghai First People’s Hospital. The overall detection rate,gender,age,location of lesion, morphology,and pathology were analyzed. Results:A total of 985 patients with colorectal polyps were recruited in this study. The overall detection rate of polyps was 48. 7% . The prevalence of colorectal polyps was significantly higher in males than in females(58. 7% vs. 38. 9% ;χ2 = 79. 788,P < 0. 01). The detection rates of colorectal polyps in different age groups were statistically significant(χ2 = 23. 820,P < 0. 01). Detection rate of polyps was significantly higher in left colon than in right colon(62. 4% vs. 37. 6% ;χ2 =190. 643,P <0. 01). The overall detection rate of protruding lesion 0-Ⅰwas 94. 5% ,and slightly elevated lesion 0-Ⅱa was 48. 3% . Detection rate of adenomatous pylops was significantly higher than non-adenomatous pylops(59. 3% vs. 47. 1% ;χ2 = 27. 326,P < 0. 01). The histological results were differed between two examination in 119 patients. Only 17. 8% of the patients operated were followed up by colonoscopy,and polyps recurred in 62. 3% of them. Conclusions:A higher incidence of colorectal polyps is found in people with high risk of colorectal cancer screening. High quality colonoscopy examination and effective operation in time can reduce the incidence of colorectal cancer.

Considering the core concept of the system theory,the author describes the conception,components and main functions of the subjects,the objects and behavioral factors for the popularization and application of appropriate health technologies.This theoretically built up the system model for transfer and application of the appropriate health technologies in Chinese rural areas.

Objective To investigate the prevalence of colorectal cancer in community people in Shanghai,and clarify the necessity of colorectal cancer screening in community residents.Methods From April 2013 to September 2014,colonoscopy examination was carried out in people who participated in colorectal cancer screening in Shanghai with positive result of fecal occult blood test or with high-risk in colorectal cancer evaluated with the risk factors questionnaire.A total of 1 990 individuals were recruited.The colonoscopic results of them were retrospectively analyzed.Chi-square test was performed for statistical analysis.Results The 1 990 individuals received colonoscopy examination aged from 50 to 92.Among the 1 990 individuals with colonoscopy examination,colorectal polyps,colorectal cancer and carcinoid were found in 1 027 cases,and the total detection rate was 51.6％.The detection rate of colorectal polyps was 48.7％ (969/1 990),and the maximum diameter of polyps was 3.5 cm.The detection rate of colorectal polyps of the male was 59.3％ (532/897),which was higher than that of the female (40.0％,437/1 093),and the difference was statistically significant (x2 =73.7,P＜0.01).The detection rate of colorectal polyps in group 50 to 60 (not include 60) years old,60 to 70 (not include 70) years old,70 to 80 (not include 80) years old and ≥80 years old was 37.5％ (139/371),48.7％ (524/1 075),55.9％ (248/444) and 58.0％ (58/100),respectively.The difference was statistically significant (x2 =31.3,P＜0.01).The detection rate of colorectal cancer was 2.7 ％ (54/1 990).The detection rate of male rectal cancer was 3.8％ (34/897),which was significantly higher than that of female (1.8％,20/1 093),and the difference was statistically significant (x2 =7.2,P＜0.05).The detection rate of colorectal cancer in group 50 to 60 (not include 60) years old,60 to 70 (not include 70) years old,70 to 80 (not include 80) years old and ≥80 years old was 1.6％ (6/371),1.9％ (20/1 075),4.3％ (19/444) and 9.0％ (9/100),respectively.The difference was statistically significant (x2 =23.7,P＜0.01).The detection rate of rectal carcinoid was 0.2 ％ (4/1 990).Conclusions The prevalence of colorectal polyps and cancer is high in screening community people with high-risk of colorectal cancer.Colorectal cancer screening in community people ≥ 50 years old with sequential screening method is important.

This paper discussed in detail the external macro factors and internal key function mechanisms of the transformation and application system for appropriate health technologies in rural areas.The macro components include political factors,social factors,population factors,environmental factors and health policy factors.The internal key function mechanisms include policy mechanism,benefit mechanism and guarantee mechanism.

Objective To analysis the risk factors and drug resistance of multidrug-resistant pseudomonas aeruginosa(MDRP) infection and to provide the basis for clinical anti-infective therapy .Methods Predisposing factors and drug resistance to clinical commonly used antibacterial drugs of MDRP which were separated from September 2010 to December 2011 were adopted for retro-spective analysis .Results A total of 235 hospital MDRP infection were collected ,97 multidrug resistant strains were concluded ,the separation rate was 41 .2% .The separation rate of ICU and neurosurgery were higher ,account for 35 .54% and 22 .31% respective-ly .The main resource of specimen is respiratory tract ,about 75 .21% .The resistance of MDRP was very serious .The resistant rate of Amikacin was the lowest ,account for 37 .11% ,followed by piperacillin/tazobactam ,ceftazidime ,account for 47 .4% and 48 .45%respectively .The resistant rate of other antimicrobial agents were greater than 50% .Advanced age ,serious underlying diseases ,long hospital stay ,long-term repeated application of broad-spectrum antimicrobial drugs ,admission to ICU ,invasive treatment operations were the risk factors for MDRP resistance .Conclusion The resistance is very serious ,in order to reduce the generation and spread of drug-resistant strains ,the hospital infection control ,drug resistance monitoring ,rational use of antimicrobial drugs and prevention of cross-infections should be strengthened .