Objective　 To study the mechanism of delaying aging process under optimal stress . Methods　 Kunming mice swam at the 18℃ water temperature for 15min everyday based upon the model of aging mice induced by D-galactose .After six weeks continuously , the ability of memory and learning , the contents of SOD and MDA were measured, at the same time ,the mitochondria membranous fluidity ,MDA's contents in mitochondria and the activity of ATPase in hippocampus were also determined.Results　 In the aging mice, a significant reduction of learning and memory ability was observed, the contents of SOD decreased , but MDA contents increased markedly, we also found a obvious declining at the ATPase activity and the fluidity of mitochondria membrane . But the optimal swimming stress could effectually antagonize the above-mentioned changes . Conclusions　 The optimal swimming stress could delay the aging process by regulating the balance between the oxidant system and the antioxidant system.

Objective To assess the equity of health resources configuration in Zhejiang,and provide references for regional health planning.Methods The Lorenz curve,Gini coefficient and Theil index were used to calculate and analyze.Results The Gini coefficient in 2009-2011 calculated by population density was between 0.330～0.343,which meant comparatively rational.The main reason of inequity configuration for hospital beds and nurses was inter-regional difference.Conclusion The Theil index could remedy the disadvantage that Gini coefficient could not reflex the total inequity was brought up by inter-regional or intra-regional difference.The health resource configuration should based on population health need and health service demand.

Objective To evaluate the research input-output efficiency of 15 medical universities,and provide references for improving synthetic research efficiency.Methods Selected research input-output key indicators,and used CCR model and SE-DEA model to statistic and analyze.Results The average value of comprehensive efficiency of 15 medical universities was 0.811,pure technical efficiency value was 0.978,scale efficiency value was 0.827.There were 5 DEA efficient universities,7 pure technical efficient universities among 10 Non-DEA efficient universities.Conclusion The total research efficiency of medical universities in our country remained to improve,and should emphasize adjusting scientific research scale.

Scientificly forecasting the health resources is the premise and foundation for making health resource planning.This paper summarized the application scope and characteristics of commonly used statistical models for health resources forecasting,introduced many S type curve prediction models commonly used in natural and social economic fields,hackled and concluded the weight calculation methods of combination forecasting models,and on this basis put forward that multivariable time series model or combination forecasting model based on single time series model and multi-linear regression equation of the predictive value should be set up for forecasting health resources,so as to provide methodological references for related forecasting research.

Objective: To investigate the efficacy and safety of oxycodone hydrochloride prolonged-release tablets and tramadol hydrochloride sustained-release tablets in the treatment of moderate cancer pain. Methods:Totally 290 cases of the patients with mod-erate pain were divided into the observation group with 148 cases and the control group with 142 cases. The observation group received oxycodone hydrochloride prolonged-release tablets, while the control group was given tramadol hydrochloride sustained-release tablets. The treatment course was 2 weeks, and the total efficiency and the incidence of adverse drug reactions( ADR) in the two groups were calculated and compared. Results:The total efficiency in the observation group and the control group was 92. 6% and 81. 7%, respec-tively, and the difference was statistically significant (P<0. 01). The incidence of ADR was 60. 1% and 57%, respectively with no significant difference (P>0.05). Conclusion: The effect of oxycodone hydrochloride prolonged-release tablets in the treatment of moderate cancer pain is better than that of tramadol hydrochloride sustained-release tablets.

BACKGROUND: Central oxytocin (OT) may be a neurotransmitter or neuromodulator and play an important role in learning and memory, sexual behaviour, pain modulation and opiate tolerance and dependence. To research the interactions between oxytocinergic and opioidergic system in hippocampus has some significance.OBJECTIVE: To investigate the effects of OT administered intracerebroventricularly on evoked discharge of left dorsal hippocampal CA1 neurons in rats and the possible interactions between oxytocinergic and opioidergic system.DESIGN: A randomised controlled study.SETTING: Department of Physiology of Guangdong Medical College; Department of Physiology and Pathology of Medical College of Wuhan University.MATERIALS: The study was conducted in the Physiology Department of Medical College of Wuhan University from September 2002 to September 2003. A total of 36 male Sprague-Dawley rats were selected and randomly divided into six groups: control (NS), OT groups (0.2 mg/L, 2 mg/L and 20 mg/L), [d (CH2)5-OVT]+OT (2 mg/L), naloxone+OT (2 mg/L), with 6 rats in each group.METHODS: Single-unit recording was performed with glass microelectrode. The glass microelectrode was inserted by a micromanipulator into hippocampal CA1. The electrical activity was amplified by a microelectrode amplifier and then recorded by the biological experimental system,monitored at the same time with oscilloscope. When recording the neural discharge, electrical stimulation of the sciatic nerves was performed once 5minutes through a double stainless electrode. 5 μL oxytocin in dosage of 0.2, 2 and 20 mg/L were injected slowly into lateral ventricle via microlitre syringe. [d(CH2)5-OVT]+OT (2 mg/L) group: 2.5 μL [d(CH2)5-OVT](80 mg/L) was injected into lateral ventricle and then 2.5 μL oxytocin (2 mg/L). Naloxone+OT (2 mg/L) group: 2.5 μL naloxone (400 mg/L) was injected into lateral ventricle and then 2.5 μL oxytocin (2 mg/L). According to frequency of discharge, effect of oxytocin at various dosages on discharge induced by neurons in hippocampal CA1 area and [d (CH2)5-OVT]and naloxone on oxytocin was assayed. MAIN OUTCOME MEASURE: Changes of discharge frequency after stimulation.RESULTS: Data of totally 36 rats were entered the final analysis. ① OT (0.2 mg/L, 2 mg/L and 20 mg/L) administered by intracerebroventricularly could decrease the evoked discharge of hippocampal CA1 neurons in a dose-dependent manner. ② The inhibitory effects of OT (2 mg/L) could be blocked by pretreated intracerebroventricularly injection of [d (CH2)5-OVT](80 mg/L, 2.5 μL). ③ Intracerebroventricular injection of naloxone (400 mg/L, 2.5 μL) could attenuate the effects of OT (2 mg/L) significantly.CONCLUSION: OT can inhibit the electrical activities of hippocampal CA1 neurons to external electrical signal through activating the oxytocin receptor. Moreover, central opioid receptor is involving in the inhibitory effects of OT.

Objective To observe the clinical curative effect of ten-day sequential therapy composed of omeprazole,amoxicillin,clarithromycin,levofloxacin in Helicobacter pylori (Hp) eradication.Methods 120 adult patients with positive rapid urease test of gastric mucosa were selected,they were randomly divided into the sequential group (60 cases) and control group (60 cases).The sequential group was treated with 10 d sequential therapy and control group was given 10 d standard therapy for eradication of Hp.In the two groups,patients with active ulcerative continued taking omeprazole for 4 weeks,the 14C urea breath test was taken after stopping drug,negative for Hp eradication.The clinical efficacy of two groups was observed.Results Hp eradication rate of sequential group was 94.8 ％,which was significantly higher than 79.3 ％ of the control group (x2 =6.20,P ＜ 0.05).The incidence rate of adverse reactions in the sequential group was 17.5％,which in control group was 15.0％,the difference was not significant (x2 =0.06,P ＞ 0.05).Conclusion Ten-day sequential therapy composed of omeprazole,amoxicillin,clarithromycin,levofloxacin for eradication of Hp is safe,the cure rate is high,and the adverse reaction is similar with standard therapy,it is worth the clinical promotion.

This paper reports the results of forensic pathological study of 128 autopsycases of sudden coronary death(SCD).The severity of the coronary arterystenosis was 4 degree in 63 cases,3 degree in 26 cases and 2 degree in 39 cases.The distribution of the artheroscleorosis of 3 and 4 degree was quite extensive. Recent thrombosis in CA was found in 18 cases,hemorrhage in plaques in 17cases.Only 2 cases had acute myocardial infarction.Inflammatory cell infil-tration were found in coronary plaques in 36 cases.Myocardail fibrosis orsmall scar formation were detected in 56 cases.It is suggested that SCD isthe commonest cause of sudden unexpected death.The majority of SCD(61%)were manhood in middle age.Most cases died suddenly during sleep withoutany clear inducements.The characteristics of the pathological changes in theCA and myoc ardium and the pathological diagnosis of SCD were analyzedand discussed.

Myoglobin (Mb)depletion from myocardium in the cases of sudden soronary death (SCD) and was firstly studied by an immunhistochemial technique (ABC method) in China. Mb was detected quantitatively using scanning microscope photmeter and the results were analyzed statistically by computer The results showed that there were marked depletion of Mb from myocardium in each case of SCD The Mb depleted arce were multiple, disseminatly and segmentally distributed while no depletion of Mb from myocardium in the cases of control group was seen We suggested that the depletion of Mb can be used as one of the diagnostic criterion in the cases of SCD.

BACKGROUND:Intraarticular hyaluronic acid (HA)has been used as one common method in the treatment of osteoarthritis. But its application in treating Kashin-Beck disease (KBD) is generalizing. The effect of HA on chondrocyte metabolism in KBD patients remains unclear.OBJECTIVE:To investigate the effects of HA on the synthesis of collagen Ⅱ and aggrecan in KBD chondrocytes cultured in vitro to understand its clinical treatment for KBD.DESIGN,TIME AND SETTING:Comparative observation. The experiment was performed in the Laboratory Center of Biomedicine and Endemic Disease Laboratory of College of Medicine,Xi'an Jiaotong University from September 2006 to January 2009.MATERIALS:Samples of KBD were from cartilage and corpus liberum excised from 6 KBD patients undergoing knee joint operation,and normal samples of fresh knee cartilages were provided by 6 people undergoing amputation or death by accident METHODS:Chondrocytes of KBD and normal control were separated and cultured in vitro. Varying dosages of HA (100,500 gm/L) were administered to normal and KBD chondrocytes cultured in vitro for 6 days. Negative control was not treated with HA.MAIN OUTCOME MEASURES:Effects of HA on collagen Ⅱ and aggrecan mRNA expression in KBD patients and normal people by RT-PCR.RESULTS:Compared with normal control group,the mRNA level of collagen Ⅱ and aggrecan were lower in KBD (P＜0.05).After administration of HA (100,500 mg/L) the mRNA level of collagen Ⅱ and aggrecan were significantly increased,and the effects of 500 mg/L was more significant.CONCLUSION:HA can increase the collagen Ⅱ and aggrecan mRNA level of KBD chondrocytes cultured in vitro. In particular,500 mg/L HA significantly promoted chondrocyte metabolism compared with 100 mg/L HA.