1.GENETIC VARIATION BETWEEN MAONAN AND MULAO ETHNIC GROUPS OF CHINA BY STR
Acta Anatomica Sinica 2002;0(06):-
Objective To investigate the 6 short tandem repeats(D2S1338,D8S1179,D16S539,D18S51,D19S433,D21S11) polymorphism in Maonan and Mulao minority and their genetic relationships with other 5 minorities in Guangxi Province and to enrich the genetic database of Maonan and Mulao populations. Methods The allelic frequencies and the genotype of six STR loci were generated from 383 unrelated Maonan and Mulao individuals by PCR_STR and ABI3100. Results Sixty_one and 65 STR alleles were found in the six STR loci of Maonan and Mulao populations,with their frequencies ranging from 0.002 5-0.320 0 and 0.002 7-0.284 2 respectively.Two hundred and sixteen and 218 genotypes were found in Maonan and Mulao populations,with their frequencies ranging from 0.005 0-0.150 0 and 0.005 5-0.158 5 respectively.Their average heterozygosities were above 0.8;polymorphism information content were above 0.8 their accumulative discrimination power were over 0.999 999 98,and the probability of paternity exclusion were over 0.998 3.The results of genetic distance and phylogenetic tree showed that there were closest genetic relationship between Maonan and Mulao minorities,and farthest genetic relationship was existed between Shui and Yi minorities.Seven minorities were clustered to 2 groups on phylogenetic tree.Yi minority was clustered to one group,whereas the other 6 minorities(Maonan,Mulao,Hui,Miao,Shu and Jing nationalities) were clustered to the other group.Conclusion Six STR loci of Maonan and Mulao minorities possesse the characteristic of high genetic diversities which has great practical value.Therefore,the obtained data can be used in human population genetics investigation,individual indentification and paternity test.The genetic variation of STR among 7 populations is basically consistent with their historical culture and geographic distribution.
2.Genetic polymorphisms of six STR loci in Maonan population in Guangxi province
Shaokang TENG ; Lin XU ; Ming LAO ; Lining ZHOU ; Qiongying DENG ; Songfeng LI ; Jichun GONG
Basic & Clinical Medicine 2006;0(07):-
Objective To investigate the distribution of six short tandem repeats(STR) loci in Maonan in Guangxi province and to study genetic relationship among 6 minorities.Methods Two hundreds unrealated individulas were analyed by STR genescanning.Genetic distance was computed and phylogenetic tree was construted for studying genetic relationship among 6 Ethnic groups.Results The average heterozygosity(H) in the six STR loci was 0.7825;the average polymorphism information content(PIC) was 0.7324;the accumulative discrimination power(DP) was 0.99999951,and the accumulative probability of paternity exclusion(EP) was 0.994575.All 6 populations were clustered into 2 group.Conclusion Allele 16 in D3S1358,allele 11 in D5S818,allele 11 in D7S820,8 in D13S317,14 in vWA,22 in FGA are probably the most primitive alleles in repective locus.The data obtained indicate highly genetic polymorphisms.Genetic relation between Maonan and Mulao were close.
3.STUDY ON POLYMORPHISMS OF 15 STR LOCI IN MIAO ETHNIC GROUP OF RONGSHUI COUNTY IN GUANGXI PROVINCE
Lining ZHOU ; Yaochun QIN ; Lin XU ; Songfeng LI ; Qiongying DENG ; Jichun GONG
Acta Anatomica Sinica 2002;0(05):-
0.05).Conclusion The 15 STR loci of Miao ethnic group show the characteristics of high polymorphism and hereditary stability.Therefore,the obtained data can be of applicable value in human population of Guangxi Miao genetics investigation,and forensic application,as well as individual identification and parent checkup.
4. Meta-analysis on safety and efficacy of dual antiplatelet therapy combining with proton pump inhibitors for patients after percutaneous coronary intervention
Qianqiu CHE ; Qiang WU ; Yubo LIANG ; Runmin SUN ; Qianwen LYU ; Junli MA ; Hao HU ; Xin LIN ; Guangli XU ; Shougang SUN ; Chun ZHANG ; Qiongying WANG ; Jing YU ; Feng BAI
Chinese Journal of Cardiology 2019;47(2):129-140
Objective:
To analyze the impact of dual antiplatelet (DAPT) therapy combining with or without proton pump inhibitors (PPI) on the main outcomes after percutaneous coronary intervention (PCI).
Methods:
The PubMed, EMBASE and Cochrane Library were searched for relevant literature and the references obtained from these sources were retrieved manually from inception till September 2017. Inclusion and exclusion criteria were established follow the Cochrane review standard. A total of 977 literatures were included, 193 duplicates were excluded, 74 reviews, case reports, letters and systematic reviews were excluded, 667 literatures were excluded after reading the title and abstract, 34 literatures were excluded due to non-randomized control studies and unrelated outcome indicators, and 9 literatures were finally included with a total of 16 589 patients. RevMan 5.3 software was used to compare the incidence of major adverse cardiovascular events (MACE), cardiogenic death, recurrent myocardial infarction, target vessel revascularization, all-cause death, stent thrombosis, stroke, gastrointestinal bleeding and gastrointestinal events in patients with DAPT combining with or without PPI after PCI.
Results:
MACE was observed in 8 out of the 9 included literatures, and the results showed that MACE occurred in 561 out of 6 282 patients receiving DAPT combining with PPI therapy and in 951 out of 9 632 patients using DAPT alone (
5.Predictive value of laboratory tests on severity of newly hospitalized patients with COVID-19
Zhenghui HUANG ; Sha XU ; Jinhua CHEN ; Qiongying WEI ; Lan LIN ; Xiangli YE ; Dan XUE
Chinese Journal of Laboratory Medicine 2020;43(10):973-977
Objective:To analyze the laboratory tests in newly hospitalized patients with COVID-19 and their predictive values for the severity of the disease.Methods:83 patients, including 54 males and 29 females, with median interquartile range of 63(53-70)years diagnosed with covid-19 who were managed by Fujian medical team from January 27, 2020 to February 20, 2020 in Wuhan JinYinTan Hospital were studied retrospectively. According to the severity of the disease, they were divided into common, severe and critical groups. White blood cell (WBC), lymphocyte (LYM), loctate dehydrogenase (LDH), interleukin-6 (IL-6), procalcitonin (PCT), serum ferritin (SF), erythrocyte sedimentation rate (ESR), hypersensitive C-reactive protein (hs-CRP) and D-Dimer (D-D) on admission were analyzed retrospectively. And the predictive value of each indicator for critical group was analyzed by Logistic regression.Results:On admission, in common, severe and critical groups, WBC (×10 9/L) values were 7.10±3.88, 7.73±3.77 and 9.07±5.61, respectively ( F = 1.315, P=0.274); IL-6 (μg/L) values were 11.76(9.42-15.18), 11.93(10.15-15.63) and 11.24(8.06-13.75), respectively( Z=0.591, P=0.744);D-D (mg/L) values were 0.70(0.48-1.12), 1.67(1.07-7.14) and 1.96(0.71-8.18), respectively( Z=3.363, P=0.186).There were no significant differences among three groups. On admission, in common, severe and critical groups, LYM(×10 9/L) values were 1.10±0.33, 0.80±0.35 and 0.66±0.32, respectively( F=11.415, P<0.001); SF(μg/L) values were 470.83±283.43, 835.66±819.43, and 1341.15±949.54, respectively ( F=7.98, P=0.001); ESR(mm/h) values were 30.76±15.70, 42.55±22.51 and 51.04±25.09, respectively( F=5.181, P=0.008); LDH(U/L) values were 314.71±105.46, 325.69±109.85 and 444.03±181.07, respectively( F=7.17, P=0.001); hs-CRP(μg/L)values were 16(7.20-19.90), 31.00(20.00-87.60) and 80.50(33.70-113.00), respectively( Z=12.185, P=0.002);PCT(μg/L) values were 0.025(0.02-0.05), 0.05(0.02-0.13) and 0.09(0.05-0.39), respectively( Z=9.694, P=0.008). There were significant differences among three groups. Pairwise comparison showed that the LYM value in common group was higher than those in severe and critical groups, and the hs-CRP values in severe and critical groups were higher than that in common group,while SF, ESR, LDH and PCT values in critical group were all higher than those in severe and common groups( P<0.05). Conclusions:On admission, WBC, LYM, ESR and LDH are helpful to predict the severity of COVID-19. Although the level of IL-6 is increased, it may not be meaningful to judge the severity of COVID-19.
6.Social support status and influencing factors among schizophrenics in remission in Northeast Sichuan
Xin YANG ; Guijun ZHAO ; Qiongying XU ; Pei HE ; Lirong GUO ; Yuanmei XU ; Yanjun CHEN ; Jijun RAN ; Yan HU
Sichuan Mental Health 2022;35(3):234-240
ObjectiveTo investigate the social support status and influencing factors of schizophrenics in remission in Northeast Sichuan, and to provide ideas for improving their social support. MethodsFrom May to September 2020, a total of 533 patients who met the diagnosis criteria of the International Classification of Diseases, tenth edition (ICD-10) for schizophrenics in remission at the mental health institutions in Guangyuan, Bazhong and Dazhou cities were selected for the survey, and patients were assessed by self-made demographic and clinical data inventory and Social Support Rating Scale (SSRS). Then the social support status of schizophrenic in remission and influencing factors were analyzed, meantime, the impact of the second round reimbursement policy of medical insurance benefits on their social support was addressed particularly. Results①The SSRS total score, objective support, subjective support, and utilization of support scores of schizophrenics in remission were lower than those of the national norm (t=5.065~30.382, P<0.01). ②Univariate analysis showed that SSRS score was relatively high among patients with female gender (t=-3.632), retired status (F=5.951), married status (F=5.951), spouse as primary caregiver (F=23.841), annual household income >5 000 yuan (F=15.892), patient's economic income (t=4.083), and outpatient or online follow-up (F=3.954), with statistically significant differences (P<0.05 or 0.01). ③The total and dimensional scores of SSRS in patients with access to the second round medical insurance reimbursement were significantly higher than those without (t=10.195~25.103, P<0.01). ④Multiple linear regression analysis denoted that gender, work status, marital status, primary caregivers, annual family income, economic income, follow-up visits and the second round medical insurance reimbursement were the factors influencing social support status of schizophrenics in remission (β=0.201~2.115, P<0.05 or 0.01). ConclusionThe social support of schizophrenics in remission in Northeast Sichuan is below the national average, furthermore, their social support levels are affected by the gender, work status, marital status, primary caregivers, annual family income, economic income, follow-up visits and the second round medical insurance reimbursement, and the second round medical insurance reimbursement may ameliorate the social support status of patients.