Objective:To analyze the effect of comprehensive nursing education on anxiety, depression, quality of life and survival of patients with hepatocellular carcinoma (HCC) after surgery.Methods:A total of 136 HCC patients were randomly divided into two groups: general nursing education group and comprehensive nursing education group. The comprehensive nursing education group carried out 12 months of health belief education, psychological care, care activities and telephone condolence. The routine nursing group received basic education for 12 months. The changes of anxiety and depression were assessed by hospital anxiety and depression scale (HADS). The quality of life (QOL) of participants within 12 months was assessed using the European research and treatment cancer quality of life questionnaire (QLQ-C30). The 3-year survival of the two groups was compared.Results:There was no significant difference between the two groups in baseline data. At the 9th and 12th month, the HADS-A score of the comprehensive nursing education group was significantly lower than that of the general nursing group ( P< 0.05). In the 12th month, the overall health of QLQ-C30 in the comprehensive nursing education group was better than that in the general nursing group ( P<0.05). The QLQ-C30 function score of the comprehensive nursing education group at the 12th month was better than that of the general nursing group ( P<0.05). There was no significant difference in QLQ-C30 symptom scores between the two groups at all time points ( P>0.05). The overall survival of the patients in the comprehensive nursing education group was better than that in the general nursing group ( P=0.026). Conclusion:the comprehensive nursing education can reduce the anxiety and depression of HCC patients after surgery, and can improve their quality of life and survival.

Objective To investigate the effects of knockdown of signal transducer and activator of transcription 5 (Stat5) on invasion and epithelial mesenchymal transition (EMT) of thyroid carcinoma TT cells.Methods Thyroid carcinoma TT cells were cultured and divided into Stat5-small interfering RNA (siRNA) group and NC-siRNA group,transfected with Stat5 siRNA and negative control NC-siRNA respectively.After transfection,the numbers of invasive cells were determined by Transwell model and the expressions of matrix metalloproteinase (MMP) family genes,EMT marker genes,angiogenesis genes in the cells were determined by fluorescent quantitative PCR.Results The numbers of invasive cells in Stat5-siRNA group were significantly lower than those in NC-siRNA group (42.39 ± 5.82 vs.64.41 ± 8.49,t =-4.784,P =0.001;51.23 ± 6.38 vs.78.54 ± 9.52,t =-5.329,P ＜ 0.001;60.35 ± 8.35 vs.98.42 ± 11.25,t =-6.076,P ＜0.001) after the transfection of siRNA for 12 hours,18 hours and 24 hours.Twenty-four hours after transfection of siRNA,the mRNA expressions of MMP1,MMP2,MMP9,MMP13,N-cadherin,Vimentin,vascular endothelial growth factor (VEGF),basic fibroblast growth factor (bFGF),angiogenin (Ang)-1 and Ang-2 in Stat5-siRNA group were significantly lower than those in NC-siRNA group (0.42 ± 0.07 vs.1.03 ± 0.15,t =-8.240,P＜0.001;0.35 ±0.06 vs.1.01 ±0.13,t=-10.307,P＜0.001;0.29±0.05 vs.1.05± 0.18,t=-9.097,P＜0.001;0.54±0.08 vs.0.98±0.12,t=-6.822,P＜0.001;0.38 ±0.06 vs.1.04±0.18,t=-7.778,P＜0.001;0.29±0.04 vs.1.06±0.12,t=-12.612,P＜0.001;0.36±0.07 vs.1.06 ±0.14,t=-10.000,P＜0.001;0.43 ±0.08 vs.0.97 ±0.12,t=-8.372,P＜0.001;0.25 ± 0.03 vs.1.03±0.12,t=-14.100,P＜0.001;0.19±0.03vs.0.99±0.13,t=-13.408,P＜0.001).The mRNA expression of E-cadherin was significantly higher than that in NC-siRNA group (2.88 ± 0.42 vs.0.98 ± 0.12,t =9.726,P ＜ 0.001).Conclusion Knockdown of Stat5 inhibits the invasion and EMT of thyroid carcinoma TT cells.