Objective To investigate the changes of serum leptin levels and the influential factors in maintenance peritoneal dialysis patients.Methods Seventy-six peritoneal dialysis patients were chosen at the time before,and 3 months,6 months,12 months,18 months and 24 months after they began the peritoneal dialysis therapy,to examine body mass index (BMI),triceps skinfold thickness (TSF),abdominal circumference,homeostasis model assessment of insulin resistance (HOMA-IR),the plasma lipid profile,and leptin in the same situation.Results For 24 months,these patients showed higher serum leptin level than the values before commencing peritoneal dialysis treatment (P ＜ 0.01).The level of leptin was positively correlated with the BMI(r =0.412,P ＜ 0.01),TSF(r =0.308,P ＜ 0.01),abdominal circumference(r =0.284,P ＜ 0.01),HOMA-IR(r =0.184,P ＜ 0.01) and TG(r =0.288,P ＜ 0.01),negatively corelated with the high-density lipoprotein cholesterol(HDL-C)(r =-0.285,P ＜ 0.01).Multiple logistic regression analysis showed that BMI (β =0.339,P ＜ 0.01),TGβ =0.157,P ＜ 0.01) and HDL (β =-0.126,P ＜ 0.05)were significant predictive factors for the changes of serum leptin levels.Conclusion Leptin maybe involve in the occurrence and the development of cardiovascular events like other metabolic parameters in peritoneal dialysis therapy.

Objective · To estimate correlation between coronary artery calcification score (CaCS) and prognosis of peritoneal dialysis (PD) patients.Methods · The clinically stable patients who had undergone PD for at least 2 months were recruited for this prospective and observational cohort study.Coronary artery calcification was assessed by multislice spiral computed tomography and was recorded according to the Agatston score. The patients were assigned to 3 groups, i.e. no calcification group (CaCS=0), low calcification group (0

Objective To investigate the effect ofmicroRNA-101(miR-101) overexpression on proliferation and metastasis of human hepatoma HepG2 cells and the molecular mechanism.Methods HepG2 cells were divided into blank group,negative control group and miR-101 transfection group.HepG2 cell line stably overexpressing miR-101 was established by lentiviral vector.The overexpression of miR-101 was detected by chemiluminescence method.The expression of vascular endothelial growth factor (VEGF) protein was detected by Western Blot.Scratch experiments was used to analyze the cell migration and the Transwell assay was used to detect cell proliferation.Results The expression of miR-101 in HepG2 cells was significantly increased after transfection with miR-101,and there was a direct targeting relationship between miR-101 and VEGF.Compared with the negative control group,the VEGF protein level in the miR-101 transfected group was significantly down-regulated,and the difference was statistically significant (P＜0.01).Moreover,the cell scratch healing ability and invasion ability were decreased in the miR-101 transfected group.Conclusions Overexpression of miR-101 can inhibit invasion and migration of human hepatoma HepG2 cells by targeting VEGF.