Objective To set up a system for computer-based image-processing for animal's locomotor activity test in sedative Chinese materia medica(CMM),Kaixin Powder,one of traditional complex prescription. Methods Combining the advantage of computer sciences,information knowledge,and imaging analyzed technique into the open field test,the data of mice locomotor activities were collected and analyzed with technique of pattern-recognition,data mining,etc.The total distance,time and speed of movement, resting time and their ratios in the open field were developed;and those various indexes in central area and peripheral area in the open field were established.The system was also verified by using Kaixin Powder. Results Those indexes were sensitive and objective for judging animal's locomotor activity.The correlation coefficient of those indexes collected by computer and manpower was over 0.9,which indicated that the system had better reproducibility and stability.Conclusion The results show that the system could provide an automatic and intelligent method for studying sedative CMM with integration for monitor,collection, analysis,storage,statistics,and output of experimental data and results.

0.05);The costs of the two were926.40yuan and1370.30yuan respectively(P

Objective By means of control test,to compare the surgical trauma and perioperative recovery between video-assisted thoracoscopic surgery(VATS) and conventional thoracotony for lobectomy.Methods Eightysix non-small cell lung cancer patients who had undergone operations from April 2010 to April 2011 were chosen.According to operative mcthods,the patients were divided into two groups:video-assisted thoracoscopic surgery group(VATS) and open chest group(OT).The protein and hormone level of serum Cortisol(Cor),growth hormone (GH),triiodothyronine (T3),tetraiodothyronine (T4),C-reactive protein (CRP) were selected as indexes to investigate the surgical trauma on non-small cell lung cancer patients.The duration of operation,quantity of lymph node dissection,chest tube duration,postoperative hospital stay,postoperative complications were chosen as indexes to measure operation result and perioperative recovery.Results Under the condition that patients undergone the same operation level(lobectomy + Systemic lymph node dissection),there was no ststistical significance in preoperative level of serum Cor,GH,T3,T4,CRP.The postoperative concentrations of serum Cor,GH,T3,T4,CRP on the 1 d,2 d,3 d and 7 d were lower in VATS group,and there were obvious statistical significance(P ＜0.01).And there were obvious statistical significance in duration of operation (P ＜ 0.01),postoperative hospital stay(P ＜ 0.01),quantity of lymph node dissection (P =0.477).There were no obvious statistical significance in chest tube duration (P =0.508),postoperative complications (P =0.787).Conclusions VATS lobectomy is able to achieve a same result for radical treatment outcone and safety to routine open chest lobectomy in patients.Compared witt routine open chest lobectomy,VATS lobectomy has less surgical trauma,much rapid recovery,it should be recommended for patients with non-small cell lung cancer.

Objective To observe the effects of neuregulin‐1 (NRG‐1)on calcium transients in mouse ventricular myo‐cytes.Methods Nine C57BL/6 mice of male were randomly dived into 3 groups :the blank control group ,NRG‐1 group ,and iso‐proterenol(ISO)group.Their hearts were removed and immediately cannulated via the aorta and retrogradely perfused with en‐zymatic isolation solution to get single ventricular cell by Langendorff system.Myocytes were loaded with the Ca2+ indicator Fluo‐4 and subjected to electrical field stimulation at 0.5 or 1 Hz by using living cells workstation.The change of fluorescene in‐tensity was recorded simultaneously in each group.Results Compared with the blank control group ,NRG‐1 group had signifi‐cantly increased Ca2+ transient amplitude ΔF/F0 (n=10 ,P<0.05) ,and reduced Ca2+ transient recovery time RT50 (n=10 ,P<0.05).There were no significant differences in Ca2+ transient amplitude ,Ca2+ transient recovery time and the time to peak be‐tween NRG‐1 and ISO groups(n=10 ,P>0.05).Conclusion NRG‐1 can increase Ca2+ transient amplitude and Ca2+ transients and reduce the time interval of Ca2+ transients.

Objective To investigate the expression of epidermal growth factor-like domain 7 (EGFL-7) in brain tis?sue of diabetes mellitus (DM) rats, and the intervene effect of amioguanidine and baicalein thereof. Methods Forty-eight SD rats were randomly divided into following four groups:control group, diabetic group, aminoguanidine group and baicalein group (n=12 for each group). The control group received only pelleted food and water. Diabetic group, aminoguanidine group and baicalein group were given high fat and sugar diet in combination with small dose of intraperitoneal injection of strepto?zotocin to establish the diabetic model. And then aminoguanidine group and baicalein group were given aminoguanidine and baicalein 150 mg/(kg · d) through oral gavaging. Diabetic group and control group were given equivalent saline through oral gavaging. After 16-week treatment, the expression of EGFL-7 proteins in rat brain was detected by immunohistochemistry and Western blotting methods. Results Results of immunohistochemistry and Western blotting assay showed that the ex?pression of EGFL-7 was significantly increased in diabetic group, aminoguanidine group and baicalein group compared with that of control group (P<0.01). When compared with diabetic group, the expression of EGFL-7 was significantly decreased in aminoguanidine group and baicalein group (P<0.01). There was no significant difference in the EGFL-7 expression be?tween aminoguanidine group and baicalein group (P>0.05). Conclusion EGFL-7 may play an important role in cerebral vascular hyperplasia and remodeling of diabetic rats. Aminoguanidine and baicalein restrained cerebral vessel growth by re?ducing the expression of EGFL-7.

This paper summarizes the experience of team training mode in nursing safety management.①Train key members,using case exercises of simulated teaching method and cross-departmental、cross-disciplinary;②The key members of nursing is responsible for the training of the medical staff,and as an observer in the department, observe the practical application of the team training mode;③Build team structure and master four important skills (communication,leadership,monitoring and mutual assistance);④Apply team training mode to nursing safety man-agement. After practice of one year,compare the incidence of safety changes of the observers and nursing adverse events before and after the training. In nursing staff cognition,the changes of team composition with leadership,vigi-lance,cooperation and effective communication,it is statistically significant for the team collaboration,security atmo-sphere,management perception,stress perception,and job satisfaction in the safety attitude scale(P<0.05),fell by 31.3％in the incidence of nursing adverse events. It is believed that the team training mode can improve the knowledge, attitude and execution of the nurses,reduce the occurrence of nursing adverse events,and ensure the patient's safety.

Polypoidal choroidal vasculopathy (PCV) is a fundus disease characterized by choroidal anomalous branch vascular network and terminal polypoidal dilatation.According to its fundus feature,lesion location,imaging feature and disease progression,PCV can be divided into different types or stages.It can be divided into hemorrhage and exudation PCV according to the fundus features,into macular,peripapillary,periphery and mixed types according to the lesion locations.It can also be divided into type 1 and 2 according to the ICGA (indocyanine green angiography) manifestations,and can be classified as early stage and late stage according to disease progression.There were different correlations between different types of PCV and some risk genetic loci,such as ARMS2 (age-related macular degeneration factor 2)/HTRA1 (high temperature essential protein A1),C2,complement factor B,complement factor H,and elastin genes.The response to therapy and prognosis are also different between different types.It is important to further study the clinical classification of PCV,to explore the genetic characteristics,influencing factors and treatment or prognosis features of different types of PCV.The results will improve the differential diagnosis of PCV,and the effectiveness of individualized treatment.

Because of the unique molecular structure and stable performance of yolk immunoglobulin ( IgY) , it has been a hot spot in the field of antibody research.As a new type of vaccine, nucleic acid vaccine has more advantages than most traditional vaccines, such as production cost, immunizing dose and immune protection.The approach combining the advantages of nucleic acid vaccine and IgY production in egg yolk and suck IgY has wide scope of application.In this re-view, we introduce a quick and inexpensive production method of IgY, namely, IgY produced by nucleic acid vaccine im-munization.

This review was made to compare the multimedia teaching results with traditional teaching results in medical universities or colleges.Two kinds of opposite viewpoints appeared:the multimedia teaching results was better or worse than the latter.

Objective To compare effect of the GlideScope and direct laryngoscopy in snoring surgery.Methods Fourty patients scheduled for orotracheal intubation of general anesthesia in snoring surgery wererandomly divided into GlideScope group ( A group) and direct laryngoscope group ( B group). Two groups were recorded values of heart rate (HR),systolic blood pressure (SBP), diastolic blood pressure (DBP)before induction (T1), after induction (T2), intubation (T3) and 1 (T4), 3 (T5), 5 (T6) min after intubation and plasma norepinephrine (NE) concentrations in T1, T5, T6. Results ( 1 ) Values of HR, SBP and DBP of two groups were compared in T1 and T2, the difference was not significant ( P ＞ 0.05 ); Values of SBP and DBP of two groups compared T1 to T2, and the difference was significant ( P ＜ 0. 05); (2) Values of HR, SBP and DBP of two groups compared in T3, T4 and T5, and the difference was statistically significant (P ＜0.05);(3 ) Values of the concentration of plasma NE of two groups compared in T1 ,T5 and T6, and the difference was statistically significant ( P ＜ 0. 05 ). Conclusion Cardiovascular response and stress response of orotracheal intubation using a videolaryngoscope is lower than using ordinary laryngoscope in snoring surgery.