Objective:To investigate the expression of Trophinin gene in the mouse endometrium during the period of blastocyst implantation.Methods:By reverse transcription-polymerase chain reaction(RT-PCR),Trophinin gene in the mouse endometrium of 1～6 day normal pregnancy was detected.Results:The Trophinin mRNA was not detected in endometrium of nonpregnant and 1～2 day pregnant mouse.But in 3～5 day normal pregnancy it was strongly expressed.The Trophinin gene was scarcely detected in the endometrium of the 6 day pregnancy.Conclusion:Expression time of Trophinin mRNA in mouse endometrium coincides with blastocyst implantation.This suggests Trophinin participate in the adhesion of blastocyst and endometrium.

Objective: To establish an in vitro screening system for testing effects of MAD2 gene of embryonic cell genome.Methods: In order to construct the plasmid expressing MAD2-GFP fusion plasmid,the target gene fragment was obtained by RT-PCR amplification and inserted into pEGFP-N1 reporter vector.The structure of constructed plasmid was confirmed by electrophoresis analysis and DNA sequencing.The function of construct was confirmed by lipofectamine-mediated transient expression in HepG2 cells.Results: DNA sequencing showed that the inserted fragment of the constructed plasmid was the same as the template MAD2 genome.The HepG2 cells transfected with the constructed plasmid could express reporter gene of gfp.Conclusions: the plasmid expressing gfp gene controlled by MAD2 gene is successfully constructed and an in vitro testing system for evaluating founction of MAD2 gene in separation of cell is established.

objective:To screen and identify the interactive proteins of Mps1 and provide clues for the studies on Mps1 function on the chromosome segregation.Methods:The human embryonic cDNA library was screened with pDBLeu-Mps1 as bait plasmid by yeast two-hybrid system.And two Leu+ and LacZ+ positive yeast clones were obtained,which were contransformed to the MaV203 along with pDBLeu-Mps1 plasmid one to one by yeast simultaneous contransformation so as to identify the protein interactions again.The inserted fragments of identiyied positive clones were sequence and analyzed with BLAST in NCBI.Results: Only one positive clone identified one to one yeast simultaneous cotransformation,and the other was negative.The positive clone fragment was MAD1 protein by BLAST analysis in NCBI.Conclusion:To screen and initially identify the interactive protein of.Mps1 this research is a foundation for the study on the founctions of Mps1.

Objective:To discuss the mechanism of down-regulated expression of MAD2 gene in trophoblastic cells by microRNA.Methods:Endogenous MAD2 mRNA level was compared by using quantitative real-time PCR before and after transfected with microRNA plasmid;and the Mad2 protein level was compared by western blot.Results:Ratio of MAD2 mRNA/GAPDH mRNA of control group was 0.1780?0.0688,and after transfected with microRNA recombinant plasmid was 0.1778?0.0689 and 0.1778?0.0670respectively.Statistical study of samples showed they have no instinct different;otherwise the protein level in one of the experimental groups was significantly decreased after transfected with microRNA plamid.Conclusion:This study established microRNA can down-regulate gene expression in mammalian cells at translation level,it is a good foundation to study on the control of gene expression.

Objective:To study the expression of mitosis checkpoint gene hsMAD2 in colorectal cancer. Methods:The expression of hsMAD2 gene mRNA in colorectal cancer and normal colorectal tissue of 18 cases was quantitatively detected by RT-PCR. Results:The ratio of hsMAD2 mRNA/ GAPDH mRNA in colorectal cancer and normal colorectal tissue were 0.1235?0.0752 and 0.0312?0.0598,respectively. The results suggested that the expression of hsMAD2 mRNA in colorectal cancer were significant higher than in normal colorectal tissue(P

Objective:To discuss the depressed expression of BUB1 gene in trophoblastic cells by microRNA.Methods:Endogenous BUB1 mRNA level was compared by using quantitative real-time PCR before and after transfected with microRNA plasmid; and the Bub1 protein level was compared by western blot.Results:Ratio of BUB1 mRNA/GAPDH mRNA of control group was 0.1960?0.0688,and after transfected with microRNA recombinant plasmid was 0.1968?0.0689 and 0.1962?0.0670 respectively.Statistical study of samples showed they have no instinct different;otherwise the protein level was significantly decreased after transfected with microRNA plamid.Conclusion:This study established microRNA can control gene expression in mammalian cells at translation level,it is a good foundation to study on the control of gene expression.

The construction of key laboratory in colleges is the important warranty of the upgrading of school's comprehensive research competence.In this text,we have discussed how to strengthen the building of key laboratory and facilitate the overall development of research system in university,and considered the essentiality of construction and the reason of development.

Objective:To analyze the failure factors in root canal therapy(RCT)by analysis of the failed cases.Methods:215 teeth of 206 patients were analyzed according to former treatment records,clinical examination,pretreatment radiographs,and root canal exploration during endodontic retreatment.Results:67.9% of failed teeth showed apical radiolucency,12.6% showed under-filled canal material without apical radiolucent area,and 8.4% involved coronal leakage.When examined with X ray,84.2% of teeth indicated under-filling,and most canals of premolars and molars had poor taper.Most missed root canals were found in maxillary first molars and premolars.18.1% of teeth had complications such as ledge and apical transportation.Conclusions:The factors associated with RCT failure involve cleaning,obturation and poor seal of root canal system resulted from inadequate shaping.Coronal leakage and missed canals also contribute to the endodontic treatment failure.

Objective In order to provide important theoretical basis for further improving design of DC-7/7A high altitude partial pressure suit and FTF-2 air ventilation suit (named as A suit and B suit), the performance of ventilating heat dissipation of DC-7/7A and FTF-2 was studied. Methods Five healthy volunteers were enrolled to took participate in A group and B group tests. Environmental weather conditions for A and B suits were set at Tdb 40℃ and 45℃,rh 45% and 40% respectively, and the test time was 90min and 120 min respectively. Results During the test for A group and B group, Ts ranged from 31.5℃ to 34.5℃ for human comfort. Average skin temperatures of the lower part of the body were from 33.1℃ to 33.8℃. Skin temperatures of the legs did not obviously change or slightly lowered. Heart rate showed an increase of 12 beats/min and 14 beats/min. Sweat quantities were measured to be 0.288?0.056kg/1.5h and 0.445kg?0.077 kg/2h respectively. Evaporation rates were 77.4?3.6% and 84.2?2.4% respectively. Subjective uncomfortable sensation was more obvious as test time went on. Conclusion The results of the ventilating heat dissipation for both groups were quite good, but the findings suggested that the number of the ventilating holes for some important sites should shall be re-considered. Pilots wearing A group and B group with ventilation function would still suffer from mild heat stress and obvious subjective discomfort in hot enviroment.

Objective: To study the isolation, identification, and biological characteristics of the secondary metabolites of myxobacteria. Methods: A growth inhibition model of Pyricularia oryzae was used to screen the active microbes. The compounds extracted with methanol from the fermentation broth of Myxococcus xanthus 095B06 were separated by silica chromatography, gel filtration chromatography, and high-performance liquid chromatography. The chemical structures of the compounds were identified by 1HNMR, 13CNMR, ESI-MS, and EI-MS techniques. The bioactive activities of the separated compounds were evaluated by Kirby-Bauer Disc Diffusion method and MTT method. Results: Six compounds, namely, Avermectin Ala, Avermectin A2a, Avermectin Bla, Avermectin B2a, ergosta-7,22-dien-3,5,6-triol, and 4-quinolinecarboxylic acid, were obtained. Conclusion: Compound 1, 2, 3, 4, and 5 have been isolated from myxobacteria for the first time and compound 2 and 4 can strongly inhibit the growth of SMMC-7721 cell line, with both IC50 values being 5 g/ml.