Objective]Conclude Huang Yajun chief physician with rich experience in the treatment of chronic pelvic inflammatory disease by traditional Chinese medicine.[Methods]By following Huang Yajun chief physician for clinical study,through the studing,observing,getting the answer from teacher,and the patient's feedback,tally up experience of teachers for the treatment of chronic pelvic inflammatory disease, accompanied by basis. [Results] Doctor HuangYajun is good at summarizing her experience, looking for rule,guiding practice with traditional Chinese medicine theory, through her thinking and summary, form a set of effective treatment.It is that she thinks the disease is a mixture of deficiency and excess, lasting for long time, will surely lead to blood stasis, blood stasis resistance the uterus,so to treat yu in the treatment of chronic pelvic inflammatory disease throughout, and accord to the accompanying symptoms of them;she also concluds deficiency of the kidney and blood stasis resistance for chronic pelvic inflammation touching refractory main pathogenesis characteristics.Most patients are able to be healed in three months, half a year without relapse.[Conclusion]Huang Yajun chief physician's experience is effective for treating chronic pelvic inflammatory disease, worth learning and promotion.

The pathogenesis of diabetic retinopathy is complicated.The vast network of multiple factors including unifying mechanism,inflammatory reaction,neuron degeneration and metabolic memory of glucose,and the four established pathogenic molecular pathways are hotspots of mechanism research for diabetic retinopathy.Nevertheless,these researches may be only one corner of the iceberg of DR mechanism,and we still face enormous challenges in DR mechanism research.Collaboration with multiple disciplines to study the relationship between DR and diabetes and other systemic diseases,search novel therapy targets may increase the result in an unexpected windfall for DR basic research.

AIM: To investigate law of vacuum drying process and provide basic technical supports for drying process and design of drying machine. METHODS: A batch-drying experiment system was used to continuously record the mass and temperature of the drying sample,then the key effective factors and characteristics of different phase of drying process were obtained based on the analysis of the recorded experimental data. RESULTS: The influence of process parameters on evaporation rate varied in different period of drying process. CONCLUSION: In order to improve the efficiency of the integrated drying process,it's necessary to apply appropriate technical methods according to the characteristics of the process.

Objective To study the relationship of HPV pseudo-neutralizing titers detected by two different reporter genes: Zoanthus sp. green fluorescent protein (ZsGreen) and secreted alkaline phosphatase (SEAP) , and the relationship between HPV the pseudovirus-neutralizing antibody titer and the antibody titer determined by ELISA method. Methods The plasmids with expression cassettes of the HPV capsid protein L1 and L2 genes after codon optimization and the plasmid with reporter gene (ZsGreen or SEAP) were co-transfected into 293FT cells. The cell lysate supernatants were collected after 48 h culture, then the pseudovirus was purified through POROS column chromatography from the supernatants. After the titer of pseudovirus bulk were measured, HPV-16 and HPV-18 pseudovirus-neutralization assays were carried out for determining the titer of sera collected from immunized mice with HPV candidate vaccine and Gardasil HPV vaccine. Results In statistical analysis, the two reporter gene systems for the detection of the pseudovirus neutralizing antibody titer are highly relevant to each other (Spearman coefficient; r = 0. 760). And their neutralizing antibody titers bear a high degree of correlation with the antibody titer (Spearman coefficient: r= 0.577 and r =0. 741). Conclusion ZsGreen and SEAP pseudovirus neutralizing antibody titers are highly relevant to each other. The neutralizing antibody and the antibody titer are also relevant. These results reveal some mechanism of HPV vaccines to prevent the virus from invading the host cells, and are absolutely useful in the protection efficiency evaluation of the HPV-16 and HPV-18 candidate vaccines.

AIM: To investigate the effectiveness of dosing technology and excipient application on solid drug's moisture-proof property and to provide a method improving the solid drug's moisture-proof property. METHODS: Based on the analysis of the moisture absorbing process and the absorbing mechanics,a new concept "active moisture absorbance site"(AMAS) was defined and mathematic model was constituted.Two constants as: initial AMAS concentration C_0,moisture resistance coemcient K,were proposed to indicate the drugs moisture-proof ability. The CI WU JIA extracts and SHUANG HUANG LIAN extracts were used to conduct experiments, which were designed to investigate the improvement of the drug's moisture resistance ability when varying the AMAS's concentration and/or its space distribution. RESULTS: Reducing the amount of moisture absorbance excipients in each dosage of solid drug can lower the drug's moisture absorbance ability,and applying a thin film coating material outside the drug can also slow the drug's moisture absorbance rate. CONCLUSION: The proposed mathematic model is useful for finding a way to improve the drug's moisture resistance;experimental data shows that the drug's moisture-proof property would be improved by reducing the amount of moisture absorbance excipient in each dosage and improving the moisture distribution resistance through a certain dosing technology,such as film coating technology.

Objective To investigate the expression pattern of Th1 and Th2 in patients with pulmonary tuberculosis including sputum positive pulmonary tuberculosis and coinfection with human immunodeficiency viruses(HIV), and its relation to disease severity.Methods The expression pattern of CD4+T lymphocytes, Th1 and Th2 cells in peripheral blood samples from sputum positive pulmonary tuberculosis patients, coinfection with HIV patients and healthy controls were studied by flow cytometry.Results The expression of CD4+ T lymphocytes and Th1 cells in sputum positive pulmonary tuberculosis patients were significantly lower than that in healthy group(31.22?9.80)%,(9.78?3.09)% vs (43.77?10.78)%,(25.26?4.73)%; The expression of Th2 cells in culture positive pulmonary tuberculosis patients was significantly higher than that in healthy group(18.65?3.49)% vs(10.15?2.60)%; The Th2 level in severe pulmonary tuberculosis patients was significantly higher than that in the medium and mild patients(21.70?2.67)% vs (14.87?1.66)% ,(17.48?2.06)%, while the CD4+T lymphocytes and Th1 levels were significantly lower; The CD4+ T lymphocytes and Th2 cells levels in pulmonary tuberculosis patients coinfection with HIV were lower than that in tuberculosis patients without HIV coinfection(21.88?3.71)%,(8.79?2.28)% vs (31.22?9.80)%,(18.65?3.49)%.Compared with the healthy group, Th1 cells level in pulmonary tuberculosis patients with or without coinfection of HIV were lower(25.21?4.73)% vs (9.39?2.65)%,(9.78?3.09)%.Conclusion Patients with sputum positive pulmonary tuberculosis showed lower expression of Th1 and higher expression of Th2,This profile was correlated with disease severity.Patients with pulmonary tuberculosis and HIV coinfection showed both of lower expression of Th1 without enhancement of the type 2 response.

Objective To develop new surgical first aid kits for combat readiness ,and increase the ability of field first aid .Methods On the basis of disposable surgical kits ,the surgical towels were improved .The water absorbing layer was made of SPA materials which inte-grated optimization for surgical use .Results The new surgical first aid kits for combat readiness have the surgical towels with strong water absorption,complete sterile materials,and short time for ready of supplies.Conclusion The new surgical first aid kits for combat readiness have the advantages of small volume ,practicability,available for surgery ,at the same time,which could keep the surgical incision dry and the temperature of patients stability and be helpful to decrease the risk of pressure ulcer and infection ,simplify work procedure .

Objective:To study the chemical components in the root of Asparagus cochinchinensis(Lour.)Merr.Methods:Chemical components of Asparagus cochinchinensis root were isolated by column chromatography on macroporous resin,Sil-gel,Sephadex LH-20,ODS,etc.and the structures of the components were identified by physiochemical and spectral analysis,such as MS,1HNMR,and 13CNMR.Results:The following 5 components were isolated and identified:diosgenin-3-O-?-D-glucopyranoside(Ⅰ),smilagenin(Ⅱ),26-O-?-D-glucopyranosyl-furost-3?,22,26-triol-3-O-?-D-glucopyranosyl(1→2)-O-?-D-glucopyranoside(Ⅲ),26-O-?-D-glucopyranosyl-furost-5,20-en-3?,2?,26-triol-3-O-\[?-?-rhamnopyranosyl(1→2)\]-\[?-?-rhamnopyranosyl-(1→4)\]-?-D-glucopyranoside(Ⅳ),and 26-O-?-D-glucopyranosyl-furost-3?,26-diol-22-methoxy-3-O-?-L-rhamnopyranosy(1→4)-O-?-D-glucopyranoside(Ⅴ).Conclusion:All the 5 compounds isolated from Asparagus cochinchinensis in this study is reported for the first time.

OBJECTIVE To explore the application of n-butyl-cyanoacrylate to treat laryngopharyngeal sponge angioma.METHODS The therapy and outcome of 19 cases of laryngopharyngeal cavernous hemangiomas during 1994.1～2005.1 were analyzed retrospectively.The age of all the patients ranged from 18 to 67 years with an average of 40.2 years.15 of the 19 cases were sclerotherapied by injection of a new type of tissue adhesive NBCA.4 of the 19 cases were undergone dissection of the lesion by surgical approach combined with local injection of NBCA.The duration of Follow-up ranged from 1 to 7 years.RESULTS Complete remove of tumor was achieved in 16 cases(84.2%);the tumor of 3 cases(15.7%) was removed incompletely,whose residual tumor was ablated by YAG laser in outpatient. CONCLUSION The cavernous hemangioma occures rarely in laryngopharyngx and usually are poorly defined. It is difficult for treatment because of dangerous bleeding and airway obstruction.In this study,CT、MR and DSA were used to define the extent of disease,and a high cure rate were achieved by NBCA injection combined with surgery and laser ablation.

Objective: By observing the effect of Kanglaite (KLT) Injection in combination with gefitinib on angiogenesis in mice with Lewis lung cancer, to explore its possible mechanism. Methods: Lewis lung cancer model was established in C57BL/6 mice. Forty model mice were randomized into 4 groups: control group, KLT treatment group, gefitinib treatment group, and KLT+gefitinib treatment group. Using immunohistochemistry and RT-PCR technology to observe and compare tumor growth and angiogenesis in each treatment group. Results: The average weight of the tumor in KLT+gefitinib treatment group was lower than those in the KLT treatment group and the gefitinib treatment group; the difference was statistically significant (P < 0.01). Judging from the inhibition rate, KLT, gefitinib, and KLT+gefitinib can inhibit the growth of the transplanted tumor, especially for the combination treatment. The microvessel density (MVD)-positive labeling index (LI) in control group, KLT treatment group, gefitinib treatment group and the KLT+gefitinib treatment group were 24.35±1.06, 18.25±1.36, 20.09±1.46 and 14.46±0.98, respectively. The MVD of the KLT+gefitinib treatment group was lower than those of the two single drug groups; the difference was statistically significant (P < 0.01). The expression levels of VEGF and KDR proteins in the KLT treatment group and the gefitinib treatment group were lower than that of the control group (P <0.05) and higher than that of the KLT+gefitinib group (P < 0.05). The expression levels of VEGF and KDR mRNAs in the KLT+gefitinib group was lower than those of the two single drug groups; the difference was statistically significant (P < 0.05). Conclusion: KLT combined with gefitinib treatment may exert synergistic effect on anti-angiogenesis, so as to obtain synergistic anti-tumor effect. Copyright © 2013 by TUMOR.