Aim To investigate the effect of Radices Paeoniae Alba extract on estrogen receptor (3 ( ERp ) and serotonin transporter(SERT) in the hippocampus of rat model of premenstrual syndrome( PMS) liver-qi inverse syndrome. Methods The rats were randomly divided into the normal group, the model group, the Radices Paeoniae Alba extract group, the fluoxetine group and the ER(3 agonist group by the vaginal smear microscopy and the open field score. The model group rats were prepared by electrical stimulation to prepare PMS liver-qi inverse syndrome. After five days of administration, the hippocampal tissues of each group were separated. ERp and SERT mRNA and protein expression in each group were determined by RT-PCR and immunofluorescence histochemical method. Results During the estrous reception period, there was no significant difference in the behavior score of the open- field test and the expression of ERp and SERT mRNA and protein in the hippocampus of each group. Estrous non-reception period; compared with normal rats, the total distance of open field test in model rats increased significantly. The expression of ER(3 mRNA in hippocampus significantly decreased and SERT mRNA significantly increased. In model group, the pyramidal cells in hippocampal CA1 and CA3 brain areas of rats were irregularly arranged, and the number of ERp positive cells and protein expression decreased. The number of SERT positive cells and protein expression were significantly up-regulated. After the Radices Paeoniae Alba extract was given, the above indexes were significantly improved, and no significant difference was found between them and fluoxetine group and ER agitator group. Conclusions Radices Paeoniae Alba extract may adjust the PMS card of diseases with inverse nega-tive emotions possibly by improving the hippocampus ER|3 and SERT mRNA and protein expression.

Background The reduced glutathione(GSH) is a water soluble antioxidant in retina and it is an important component of thiol.Diabetes induce the oxidative response of retina.It is very important to detect the content of thiol in retina for the evaluation of its antioxidant role.Objective This study was to investigate the effect of carnosine,aspirin and a combination of carnosine and aspirin eye drops on the change of the thiol contents in the retina of the diabetic rats. Methods Diabetic models were induced in 76 clean male Sprague-Dawley(SD) rats by intraperitoneal injection of streptozotocin(65 mg/kg).The 0.02 mi sodium citrate buffer was injected in 12 control rats.1%camosine eye drops or 0.05%aspirin eye drops were topically administered respectively in carnosine group (19 rats)or aspirin group(19 rats)two times per day for a period of 8 weeks,and the combination of 1%carnosine eye drops and 0.05%aspirin eye drops was used in the combination group at the same way(19 rats).At the end of 8 weeks.The animMs were sacrificed and the eyeballs were enucleated for the detect of thiols in the retina by colorimetry.Results The body weight of SD rats was significantly reduced and the blood glucose level was evidently arose in model group,camosine group and aspirin group in comparison with control group(P＜0.05),but those of combination group compared with control group was insignificantly changed(P＞0.05).The level of thiol in retina was(1 90.35+46.62)ms/gprot in model group,showing a considerable decrease in comparison with control (202.77+53.49 ms/gprot)(P＜0.05).However,the thiol level rose to(378.40±165.06)mg/gprot in camosine group and(351.88±211.62)mg/gprot in aspirin group,showing a statistically significant difference in comparison with control group(P＜0.05).The content of thiol in combination group compared with control group Was insignificant changed(166.972±50.287 mg/gprot us 202.77±53.49 mg/gprot).Curiously the levels in the combination group were not different from the normal control group. Conclusion The carnosine and aspirin eye drops may protect the retina against the oxidative damage caused by diabetes.

Along with the spread of human immunodeficiency virus 1 (HIV-1) infection in the world and the emergence of drug-resistant viral strains, it is urgent to seek the novel potent therapies. Chemokine receptor 5 (CCR5) is one of the main coreceptors involved in the entry of HIV-1 into target cells. Nowadays, a number of CCR5 antagonists have been developed and some of them have progressed to clinical trials or been approved. Research progress has also been made in the CCR5-targeted gene therapy. This review summarizes the recent research progress on the CCR5-targeted drug and gene therapy.
CCR5 Receptor Antagonists ; HIV Infections ; drug therapy ; genetics ; metabolism ; pathology ; HIV-1 ; drug effects ; Humans ; Molecular Targeted Therapy ; methods ; Receptors, CCR5 ; deficiency ; genetics ; metabolism

OBJECTIVE: It's practically significant to study college students' behavior and psychology in using the Internet so as to guide them to take advantage of the Internet properly and to promote the proper use of the Internet. DATA SOURCES: The related articles indexed in the Medline Databank from January 2000 to January 2004 were searched by using computer with the index words "students' psychology, computer communication netware, elements analysis", and the language of the articles was limited to English. Meanwhile, the related articles indexed in the Full-length Chinese Journals Databank, Wanfang Databank and the Journal of Chinese Clinical Rehabilitation from January 2000 to January 2004 were searched through the computer with the index words "students' behavior, internet behavior, analysis of elements ", and the language of the articles was limited to Chinese. The subjects of the study were the Chinese college students aged 18 -24.STUDY SELECTION: The materials were studied first to choose the literature on the analysis of the collage students' Internet psychology and behavior. Inclusion criteria: ① random clinical trials; ② Aged 18-20. Exclusion criteria: ① Obviously not random clinical trials; ② Repeated study, syntheses, and Mata analysis articles. For the articles that we found, we conducted a search for each full-length article.DATA EXTRACTION: A total of 81 articles accorded with the standards were involved.DATA SYNTHESIS: From the comprehensive analysis of involved articles, we found that more college students' use the web just for communication, entertainment, excitement, and spiritual sustenance.CONCLUSION: This paper analyzes the modern college students' behavioral and psychological characteristics in Internet use from the perspectives of self-awareness, mood and emotion motivation, sexual psychology and so on respectively. It also puts forward an educational policy on how to instruct the college students to make use of the Internet properly and overcome the problems of psychology towards the Internet.

Multiple organ dysfunction syndrome MODS (MODS) is the one of the major factors,which causing the death of the severe patients.More and more research proved that there are some relatonship be-tween MODS with the cellular apoptosis and necrosis.In recent years mesenchymal stem cells has been ap-plied to a variety of diseases as seed cells in the field of rehabilitation,and shows the initial effect in treat-ment and research.Through analysis the distribution and division of mesenehymal stem cells in the body,we found it may play an important role in the treatment in the MODS.We reviewed the mechanism of the MSC dealing with the MODS.

Objective To study the bone marrow-derived mesenchymal stem cell(MSC) potential as seed cells for treatment of multiple organ dysfunction syndrome(MODS) in rabbit model, lay fundamental for clin-ical utilization of the MSC for treatment of MODS. Methods MODS rabbit model was established by hemor-rhagic shock combined with endotoxin, isolation and characterization of rabbit bone marrow mesenchymal stem cells labeled MSC with GFP by lentivirus transfection, administration of MSC by ear margin vein injec-tion, MSC integration determined by PCR and pathological section, the MSCs effect on MODS rabbits evalu-ated by physical observation. Results Compared with controls, ransplanted MSCs were found in liver, lung, kidney, MODS rabbits were physically improved obviously. Conclusions MSCs are able to integrate into host without any rejection reaction, and capable of ameliorating MODS evidently.

Objective To study the expressions of α1-AT and VEGF-C in human bronchoalveolarcarcinorrm, and the relation of the expression to the patholo~cM differentiation and clinical stage. Methods All 49 Darffin embedding samples of patients with bronchoalveolar carcinoma were studied. α1-AT and VEGF-C were detected by immunohistochemical SP method.Automated image analyzer was used to quantify α1-AT and VEGF-C expressions.Results The immunohistochemical positive stainings of α1-AT and VEGF-C in brown or dark brown were located in cytopla8m.The expression levels of α1-AT and VEGF-C were not related with the gender,age,tumor position and size,and histology subtypos(P＞0.05).It Was found that the expression of α1-AT in patients with local lymph node metastasis was significantly lower than those without node metastasis(P＜0.001).It was found that the expression of VEGF-C in patients with local node metastasis significantly higher than th08e without node metastasis(P＜0.001).There Was a negative correlation between the expression level of α1-AT and the expression level of VEGF-C in bronchoalveolar carcinoma(r=-0.324,P＜0.05).Conclusion α1-AT and VEGF-C could be secreted by bronehoalveolar carcinoma.Bronehoalveolar carcinoma with lower α1-AT expression and higher VEGF-C expression is more likely to have lymph node metastasis.Lower α1-AT expression and higher VEGF-C expression can participate in the mechanism of lymph node metastasis in carcinoma together.

In order to adapt to the demand of culturing high quality talents and to strengthen student's comprehensive quality,pathophysiology department of medical college of Qinghai University carried on the reform on the pathophysiological experiment examination system.After researches and practices in recent years,we established a set of relatively sound examination system including experiment operating and experiment report in normal study,report for designable experiment and experiment skill and theory exam.Investigation results demonstrated that examination system after reform functioned well in training the students' operation ability,observation ability and ability to analyze and solve questions.

Objective To setup a platform for genetic diagnosis of Cryopyrin associated periodic syndrome (CAPS) and to use it for clinical diagnosis.Methods The peripheral blood cells of the patient and the controls were collected for DNA extraction.Nine exons of CAPS associated gene NLRP3 were amplified using polymerase chain reaction (PCR) and subjected to sequencing.Blast was used to compare the sequencing results with the reference gene and to locate mutation.The clinical information of the patient was collected and the relevant literature was reviewed.Results We set up a platform for exon sequencing of NLRP3 gene.Using this platform,we identified a nonsynonymous mutation in a female patient (D303N,aspartic acid at locus 303 mutated to asparagine).Considering the clinical manifestations of the patient,chronic infantile neurologic cutaneous and articular syndrome (CINCA) was diagnosed.Conclusion The set up of the platform for NLRP3 genetic analysis will facilitate the clinical awareness and research on CAPS.

Objective To investigate the interventional effect of multidimensional strategy to reduce the incidence of neonatal ventilator-associated pneumonia.Methods The patients who were admitted to the NICU department and received mechanical ventilation (MV) for more than 48 hours from October 2012 to September 2014 were recruited. The control group received the experienced interventions from October 2012 to September 2013, neonates from October 2013 to September 2014 were recruited as the intervention group receiving multidimensional controlling strategy, including bundle care, education, pro-cess and outcome surveillance and feedback on the practices. The compliance of before-after implementation of interventions were quantitatively evaluated,and the rate of VAP was compared between the two groups.Results The compliance rate of hand hygiene and the qualiifed rate of sputum suction, oral care, drain condensation from ventilator circuit, semi-recumbent posi-tion, and preventing of stress-ulcers were increased 17.0%, 11.4%, 14.7%, 18.2%, 37.5% and 56.3% respectively after implemen-tation of multidimensional strategy, and had statistical difference (χ2=36.47-294.36,P<0.01). But the qualiifed rate of antibiotic use only was 66.1% in the post-VAP bundle phases, and showed no statistical difference before and after(P>0.05). The VAP rate was 41.7 cases per 1000 MV-days during control group and 19.7 cases per 1000 MV-days during intervention group, had statis-tical signiifcance (P<0.05). But the rate of ventilator application showed no statistical difference between two group (P>0.05). ConclusionThe multidimensional strategy can effectively prevent the incidence of VAP.