1.Changes of NSE and NO/NOS in serum and cerebrospinal fluid after convulsion in children and the clinical significance
Journal of Third Military Medical University 2003;0(16):-
Objective To explore the relationship of neuron injury and its severity with the cause and the duration of convulsion Methods Patients were divided into 5 groups or 3 groups according to the causes of convulsion or duration of convulsion in children The levels of neuron specific enolase(NSE) and nitrogen monoxide and nitricoxide synthase(NO/NOS) in serum and cerebrospinal fluid(CSF) were tested by ELISA and spectrophotography at different time points after convulsion in children Results The levels of NSE and NO/NOS in serum and CSF of all objective groups except the febrile convulsion group were apparently higher than those in control group( t =15 4~47 7, P
2.The effect of the′cocktail′wet compress on the prevention of arm swelling after trans radial percutaneous coronary procedures
Ping TANG ; Qiong HUANG ; Hongyan REN
Chongqing Medicine 2015;(30):4199-4200
Objective To test the effect and safety of′cocktail′wet compression on alleviate the swelling arm after the inter‐vention through the radial artery .Methods Patients whose arm were swollen after the intervention through radial artery were di‐vided into two groups (experiment group ,47 patients vs .control group ,46 patients) .The experiment group adopt the′cocktail′wet compress plus traditional therapy (including elevate and immobile the swelling arm);the control group only use the traditional ther‐apy .The levels of pain ,swelling index and the number of the blister were compared between two groups .Results None of the limbs necrosis was observed in two groups .The level of pain(2 .62 ± 0 .86 vs .5 .46 ± 1 .02) ,swelling index (3 .3% ± 2% vs .5 .8% ± 3% ) of the arm ,and the number of blister (0 vs .4) in experiment group were lower than in the control group (P<0 .05) .Conclusion The bundle of therapy strategy which named'cocktail'wet compress can lessen the swelling of the arm induced by intervention through radial artery ;it is both safe and effective thus deserves widely application .
3.Clinical manifestation and histopathologic features of linear cutaneous lupus erythematosus: a report of six cases
Yan LUO ; Xiaojian LI ; Qiong HUANG
Chinese Journal of Dermatology 2010;43(6):402-404
Objective To summariza the clinical and histological features of linear cutaneous lupus erythematosus (LCLE).Methods Six cases of LCLE were analyzed retrospectively.Results LCLE was often clinically characterized by unilateral.zonal or linear,dark-erythematous patches with telangiectasia,and some lesions were covered with adherent Scales.Histologically,there were patchy perivascular and periadnexal lymphoid inflammatory infiltrate occurring in the superficial and deep dermis.Some cases were pathologically manifested by parakeratosis of superficial dermis,follicular plugging and hydropic degeneration of the basal layer of the epidermis,which was characteristic of discoid lupus erythematosus.The preferred treatment was chloroquine for LCLE.Low dose of corticosteroids were recommended for widespread lesions,especially for those pathologically manifested by lymphocyte infiltrates in the lower dermis and fat tissue.Conclusion The diagnosis of LCLE should be based on clinical and histopathological manifestations.
4.Progress of genome-wide association studies in type 2 diabetes mellitus
Qiong HUANG ; Jiye YIN ; Zhaoqian LIU
Chinese Journal of Endocrinology and Metabolism 2010;26(5):432-436
Genome-wide association studies ( CWAS) use high-throughout genotyping technologies to investigate the relation of hundreds of thousands of gene markers(genotype) with clinical conditions and measurable traits (phenotype). Type 2 diabetes mellitus results from the interaction of environmental factors with genetic variants. Many progresses have been acquired from GWAS. New gene regions have been discovered to be involved in the development and function of islet (3-cells, which provides new strategies for the etiology investigation, prevention, and treatment of type 2 diabetes mellitus.
5.Correlation and expression of COX-2 and P53 protein in basal cell carcinoma of eyelid.
Zhixiong, CHEN ; Jiong, YANG ; Qiong, HUANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2009;29(3):383-6
The correlation between the expression of COX-2 and p53 protein in basal cell carcinoma (BCC) of eyelid and apoptosis was investigated. Specimens of BCC were collected from 40 cases (aged 28-68 y) at the Department of Pathology, Renmin Hospital of Wuhan University, and Department of Pathology, Zhongnan Hospital of Wuhan University during from 1999 to 2006. Five specimens of paracancerous tissues served as control group. Immunohistochemical staining was performed to detect the expression of COX-2 and p53 in the tissues. The average absorbance (A) and the average positive area rate of COX-2 and p53 protein were measured by image analysis. The positive area rate of COX-2 and p53 protein was analyzed by linear correlation analysis. It was found that COX-2 and p53 proteins were highly expressed in BCC of eyelid, and weakly expressed in paracancerous tissues. Image analysis revealed that the expression of COX-2 and p53 proteins in BCC of eyelid was significantly higher than that in paracancerous tissues (P<0.01). Spearman rank correlation analysis demonstrated a positive correlation between the expression of COX-2 and p53 (r=0.113, P=0.421). It was concluded that COX-2 can increase the expression of p53 protein, therefore suppressing apoptosis.
Apoptosis
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Carcinoma, Basal Cell/*metabolism
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Cyclooxygenase 2/*metabolism
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Eyelid Neoplasms/*metabolism
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Tumor Suppressor Protein p53/*metabolism
6.Changes of NSE and D-dimer in blood from neonates with hypoxic ischemic encephalopathy and their chinical values
Qiong CHEN ; Zhi HUANG ; La LIU
Journal of Third Military Medical University 2003;0(10):-
Objective To detect the variation of neuron specific enolase (NSE) and D dimer in blood from neonates with hypoxic ischemic encephalopathy (HIE), and to explore the correlation of NSE and D dimer with brain damage. Methods NSE and D dimer levels in blood from neonates with HIE were measured by ELISA and turbidimetry before and after therapy. Results Blood NSE and D dimer levels in all objective cases were significantly higher than those in the control cases at 1 d after HIE and 5 d after therapy ( t =2.18-15.17, P
7.Correlation Study Between Meteorological Factors and the Number of Daily Heart Failure Patients in Emergency Room at Fengxian District of Shanghai
Jianhua HUANG ; Qiong ZHANG ; Jiangwei MA
Chinese Circulation Journal 2016;31(10):989-992
Objective: To analyze the relationship between meteorological factors and the number of daily heart failure (HF) patients for emergency room (ER) visit at Fengxian district of Shanghai in order to provide the experience for prevention and control of HF morbidity. Methods: Daily number of HF patients treated in ER of our hospital from 2012-01 to 2014-12 with corresponding meteorological, air pollution data at the same period were collected and analyzed by Spearman correlation study and multiple stepwise regression analysis. Results:①The differences of temperature, humidity, air pressure at 24-, 48-hour before and 48-hour after ER visit were positively related to the number of HF patients; the number of HF patients was signiifcantly related to the differences of temperature and air pressure at 48-hour before ER visit,P<0.001.②Multiple stepwise regression analysis showed that the most affecting meteorological factor for ER visit of HF patients was the daily lowest temperature,P<0.05, the second factor was the daily average relative humidity,P<0.05. Conclusion: Radical changes of temperature, relative humidity and air pressure elevated the number of HF patients for ER visit; daily lowest temperature and decreased relative humidity were the important factors for elevating the number of HF patients for ER visit.
8.GC-MS Analysis of Essential Oil from Mosla Chinensis Seeds
Rengeng SHU ; Haowu HU ; Qiong HUANG
China Pharmacy 2001;0(09):-
OBJECTIVE:To analyze the chemical constituents of the essential oil extracted from Mosla chinensis seeds. METHODS: The essential oil was extracted from Mosla chinensis seeds by steam distillation method and identified by GC-MS, and its relative content was determined using normalization method. RESULTS: A total of 20 chromatographic peaks were detected and 17 chemical compounds were identified, which made up 99.06% of the total essential oil content. The major chemical constituents were carvacrol(57.01%), thymol(30.72%), thymol acetate(2.56%), carvacrol acetate(2.14%) and ?-caryophyllene(1.62%). CONCLUSION: The results in our study provides scientific basis for further exploitation and utilization of Mosla chinensis and serves as reference for establishing quality standard of Mosla chinensis herbal medicine.
9.Effect of nitric oxide on myocardial cell injury after hypoxia in rats
Ming ZHANG ; Yuesheng HUANG ; Qiong ZHANG
Journal of Third Military Medical University 2003;0(11):-
Objective To clarify the effect of NO on hypoxic myocardium in rats.Methods Myocardial cells from neonatal Wistar rats aged 1-2 days cultured for 4-6 days,then pretreated with GSNO(30,200,4 000 ?mol/L)were exposed to normoxia(N)or hypoxia(H)(1%O2,5%CO2 and 94%N2)for 4 h.The NO content and LDH in the supernatant were detected.Results NO of 1.43,7.2 and 70 ?mol/L were detected respectively in the supernatant of hypoxic myocardial cells treated by GSNO(30,200 and 4 000 ?mol/L).Compared with normoxic group,LDH activity increased and myocardial cell activity decreased markedly in H+G30 and H+G4000 groups but less injury was observed in H+G200 group.Conclusion NO of certain concentration can markedly abate myocardial cell injury induced by hypoxia.
10.Analysis on 36 Cases of ADR Caused by Influenza A H1N1 Vaccine in Our Hospital
Hanbo XU ; Qiong ZHANG ; Dina HUANG
China Pharmacy 1991;0(06):-
OBJECTIVE: To probe into the incidence rate of ADR and safety of influenza A H1N1 vaccine. METHODS: 228 of medical staff of our hospital was vaccinated influenza A H1N1 vaccine on Nov. 24th in 2009 and followed up at the following day. 36 cases ADR were collected and statistically analyzed in respects of gender, age, the classification of ADR, organs or system involved and clinical manifestation, prognosis of ADR. RESULTS: The incidence rate of ADR was 15.79% versus 14.52% in the instruction. ADR were mostly occurred in nervous system (42.86%). Of 5 persons in same department, ADR occurred in four persons whose vaccines were kept at constant temperature for five hours; ADR didn’t occurred in only one person who were vaccinated in time. Incidence rate of ADR were 16.33% for female and 14.81% for male. Severe ADR accounted for 0.44% of total ADR and all cases were cured and improved. Of 36 ADR cases, severe ADR appeared in one of six patients who had allergic history. CONCLUSION: The influenza A H1N1 vaccine should be strictly used and stored according to drug instruction. People who are hypersensitive to drugs should be vaccinated carefully and observed closely after vaccination. Most of vaccination is safe although some slight and short-term ADR will appear.