Objective: To investigate the association between variable number of tandem repeat (VNTR) polymorphism in 5'-untranslation region (5'-UTR) of thymidylate synthase (TS ) gene and the efficacy of pemetrexed-based treatment in non-small cell lung cancer (NSCLC). Methods: Peripheral blood samples were collected from 90 patients with advanced NSCLC and the genomic DNAs were extracted from these blood samples. The VNTR polymorphism in 5'-UTR of TS gene was detected by fluorescent labeled PCR and multiplex short tandem repeat polymerase chain reaction in combination with capillary electrophoresis. Results: The homozygosis (3R/3R and 2R/2R) and heterozygosis (2R/3R and 3R/4R) of VNTR polymorphism in 5'-UTR of TS gene were not associated with the efficacy of pemetrexed. The progression-free survivals, disease control rates and the objective response rates in patients harboring homozygosis (3R/3R and 2R/2R) and heterozygosis (2R/3R and 3R/4R) were 112 and 94 d (P = 0.549), 64.3% and 67.6% (P = 0.745), and 12.5% and 8.8% (P = 0.591), respectively. The patients harboring 2R alleles had similar clinical outcomes as compared with those harboring 3R alleles (progression-free survival: P = 0.518; disease control rate: P = 0.631; objective response rate: P = 0.541). Conclusion: TS gene VNTR polymorphism may not be associated with clinical outcomes of advanced NSCLC treated with pemetrexed. Copyright © 2012 by TUMOR.

Objective To analyze post-traumatic growth of Chinese caregivers of dementia patients and to explore the caregivers′positive and potential energy, in order to formulate more perfect nursing intervention measures. Method Useing phenomenological method of qualitative research in selecting 6 dementia caregivers in-depth interviews to understand possible posttraumatic growth. Result Five major themes were found: changes in individual cognitive, changes in relations with others, promote their children′s education, reflections and concerns on status of pension-related changes in philosophy of life. Conclusions Dementia caregivers also have the positive performance of posttraumatic growth, in addition to the negative experience. Clinical nurses should actively explore the potential and power from the growth perspective. This is conducive to the caregivers with more health caring for life, so as to improve the quality of care, and ultimately improve the quality of life.

Atherosclerosis is a chronic inflammatory immune disease,which is one of the causes of human lower limb disability.Dendritic cells are the most powerful professional antigen presenting cell in immune system,and dendritic cells have the dual role of immunogenicity and tolerability.As a professional antigen presenting cell,the immunogenicity of dendritic cells present self abnormal antigen and course immune system disorder,which may be one role of dendritic cells participate in atherosclerosis.However,the tolerability of dendritic cells can course the generation of immune tolerance,which makes dendritic cells exert a regulatory role in atherosclerosis.This paper makes an introduction about how dendritic cells are involved and play a regulatory role in the pathogenesis of atherosderosis.

Objective To investigate the clinical significance of detection of growth differentiation factor 3(GDF3), prostate specific antigen(PSA) combined with mucin 1(MUC1)for diagnosis of breast cancer.Methods50 cases of breast cancer were selected as the breast cancer group, 50 cases of benign breast disease were selected as the benign breast disease group, and 50 healthy people were selected as the normal group.GDF3, PSA, MUC1 and tumor markets carbohydrate antigen(CA)15-3,CA125,carcinoembryonic antigen(CEA) were detected, and sensitivity, specificity and accuracy were analyzed.ResultsCompared with benign breast disease group and normal group, GDF3, PSA and MUC1 in breast cancer group were higher(P<0.05), CA15-3, CA125 and CEA were higher(P<0.05).The sensitivity, specificity and accuracy of PSA+MUC1 were higher than other group(P<0.05),the sensitivity, specificity and accuracy of combined detection of GDF3, PSA and MUC1 were higher(P<0.05).ConclusionDetection of GDF3 combined with MUC1 and PSA for early diagnosis of breast cancer has more diagnostic significance.

OBJECTIVE:To analyze the chemical constituents of the essential oil extracted from Mosla chinensis seeds. METHODS: The essential oil was extracted from Mosla chinensis seeds by steam distillation method and identified by GC-MS, and its relative content was determined using normalization method. RESULTS: A total of 20 chromatographic peaks were detected and 17 chemical compounds were identified, which made up 99.06% of the total essential oil content. The major chemical constituents were carvacrol(57.01%), thymol(30.72%), thymol acetate(2.56%), carvacrol acetate(2.14%) and ?-caryophyllene(1.62%). CONCLUSION: The results in our study provides scientific basis for further exploitation and utilization of Mosla chinensis and serves as reference for establishing quality standard of Mosla chinensis herbal medicine.

OBJECTIVE: To provide reference about the way to consummate hospital drug quality management system and ensure drug quality and patients' medication safety.METHODS: Efficient drug maintenance measures were explored by analyzing and summarizing the specific work and conservation procedures of drug maintenance in our hospital which included the humidity of storehouse,monitoring of storage condition,color-code management,expiration date management etc.RESULTS & CONCLUSIONS: Drug maintenance should be aimed at guaranteeing the drug quality;moreover,great importance should be attached to regular quality examination and maintenance of drugs and take timely effective measures aimed at the problems presented so as to ensure the drug quality,safety and effectiveness.

Clarithromycin ; therapeutic use ; Humans ; Lymphoma, B-Cell, Marginal Zone ; drug therapy ; Male ; Middle Aged ; Tomography, X-Ray Computed

OBJECTIVE To study the distribution of drug-resistant gene of meticillin-resistant coagulase negative Staphylococcus(MRCNS) in children septicemia.METHODS The total MRCNS isolates were 40,and whether in there harbored genes mec,erm and qac was studied.RESULTS Among 40 CNS strains,in there harbored genes mec,erm,and qac were 38(95.0%),30(75.0%) and 18(45.0%),respectively.CONCLUSIONS MRCNS in children septicemia where harbor drug resistance genes is very serious,so we should pay great heed to its effective control.

Aim To explore the effects of inhibitors of glycosphingolipid biosynthesis D-PDMP on the proliferation and differentiation of neural stem cells. Methods Neural stem cells cultured in vitro were treated with different concentration of D-PDMP. The effects of D-PDMP on proliferation and differentiation of neural stem cells were evaluated by counting of neurospheres size and cellular number, MTT assaying and the experiment of inducing differentiation.Results Neurospheres were smaller in size after treated with D-PDMP. The data of cellular counting and MTT assay suggested that D-PDMP could inhibit proliferation of neural stem cells and lead cells death in higher concentration, D-PDMP also reduced the differentiation ability of neural stem cells. Although differentiated cells were fewer, they could be induced into neurons and astrocytes.Conclusion The inhibitors of glycosphingolipid biosynthesis D-PDMP could inhibit the proliferation of neural stem cells, even lead cells death. It also reduced the differentiation ability of neural stem cells.

Objective To explore the atopic condition of children with stable asthma and their parents by detecting total IgE concentration in serum and collecting history. Methods Fifteen children with asthma in remission stage and 24 parents and 40 normal children were involved in this study. The concentrations of serum total IgE were measured with UniCAP Pharmacia system. The history including eczema and allergic rhinitis and asthma of children and parents were collected. Skin test with 10 kinds of inhalant allergens were taken in children with asthma. Results Of 15 children with asthma, 13 cases (86.7%)had eczema in infant stage and allergic rhinitis, 13 parents(86.7%)had allergic rhinitis or asthma. There was significant difference in the stable asthmatic children compared with parents and normal control group increased amounts of serum total lg IgE(F=68.42 P=0). There was significant difference in serum total lg IgE in patients group(2.43?0.73)kU/L compared with that in normal control group(0.72?0.54)kU/L (q=14.176 P0.05). Twelve children were positive in skin test, which were mainly dermatophagoides pteronyssinus and dermatophagoides farinae.Conclusions There is obvious congregate phenomena of atopy in the family of asthmatic children. The continuous high IgE concentration was associated with immune pathological mechanisms of atopic diseases.