Objective: To investigate the association between variable number of tandem repeat (VNTR) polymorphism in 5'-untranslation region (5'-UTR) of thymidylate synthase (TS ) gene and the efficacy of pemetrexed-based treatment in non-small cell lung cancer (NSCLC). Methods: Peripheral blood samples were collected from 90 patients with advanced NSCLC and the genomic DNAs were extracted from these blood samples. The VNTR polymorphism in 5'-UTR of TS gene was detected by fluorescent labeled PCR and multiplex short tandem repeat polymerase chain reaction in combination with capillary electrophoresis. Results: The homozygosis (3R/3R and 2R/2R) and heterozygosis (2R/3R and 3R/4R) of VNTR polymorphism in 5'-UTR of TS gene were not associated with the efficacy of pemetrexed. The progression-free survivals, disease control rates and the objective response rates in patients harboring homozygosis (3R/3R and 2R/2R) and heterozygosis (2R/3R and 3R/4R) were 112 and 94 d (P = 0.549), 64.3% and 67.6% (P = 0.745), and 12.5% and 8.8% (P = 0.591), respectively. The patients harboring 2R alleles had similar clinical outcomes as compared with those harboring 3R alleles (progression-free survival: P = 0.518; disease control rate: P = 0.631; objective response rate: P = 0.541). Conclusion: TS gene VNTR polymorphism may not be associated with clinical outcomes of advanced NSCLC treated with pemetrexed. Copyright © 2012 by TUMOR.

Objective To analyze post-traumatic growth of Chinese caregivers of dementia patients and to explore the caregivers′positive and potential energy, in order to formulate more perfect nursing intervention measures. Method Useing phenomenological method of qualitative research in selecting 6 dementia caregivers in-depth interviews to understand possible posttraumatic growth. Result Five major themes were found: changes in individual cognitive, changes in relations with others, promote their children′s education, reflections and concerns on status of pension-related changes in philosophy of life. Conclusions Dementia caregivers also have the positive performance of posttraumatic growth, in addition to the negative experience. Clinical nurses should actively explore the potential and power from the growth perspective. This is conducive to the caregivers with more health caring for life, so as to improve the quality of care, and ultimately improve the quality of life.

Atherosclerosis is a chronic inflammatory immune disease,which is one of the causes of human lower limb disability.Dendritic cells are the most powerful professional antigen presenting cell in immune system,and dendritic cells have the dual role of immunogenicity and tolerability.As a professional antigen presenting cell,the immunogenicity of dendritic cells present self abnormal antigen and course immune system disorder,which may be one role of dendritic cells participate in atherosclerosis.However,the tolerability of dendritic cells can course the generation of immune tolerance,which makes dendritic cells exert a regulatory role in atherosclerosis.This paper makes an introduction about how dendritic cells are involved and play a regulatory role in the pathogenesis of atherosderosis.

Clarithromycin ; therapeutic use ; Humans ; Lymphoma, B-Cell, Marginal Zone ; drug therapy ; Male ; Middle Aged ; Tomography, X-Ray Computed

Objective To investigate the clinical significance of detection of growth differentiation factor 3(GDF3), prostate specific antigen(PSA) combined with mucin 1(MUC1)for diagnosis of breast cancer.Methods50 cases of breast cancer were selected as the breast cancer group, 50 cases of benign breast disease were selected as the benign breast disease group, and 50 healthy people were selected as the normal group.GDF3, PSA, MUC1 and tumor markets carbohydrate antigen(CA)15-3,CA125,carcinoembryonic antigen(CEA) were detected, and sensitivity, specificity and accuracy were analyzed.ResultsCompared with benign breast disease group and normal group, GDF3, PSA and MUC1 in breast cancer group were higher(P<0.05), CA15-3, CA125 and CEA were higher(P<0.05).The sensitivity, specificity and accuracy of PSA+MUC1 were higher than other group(P<0.05),the sensitivity, specificity and accuracy of combined detection of GDF3, PSA and MUC1 were higher(P<0.05).ConclusionDetection of GDF3 combined with MUC1 and PSA for early diagnosis of breast cancer has more diagnostic significance.

OBJECTIVE:To analyze the chemical constituents of the essential oil extracted from Mosla chinensis seeds. METHODS: The essential oil was extracted from Mosla chinensis seeds by steam distillation method and identified by GC-MS, and its relative content was determined using normalization method. RESULTS: A total of 20 chromatographic peaks were detected and 17 chemical compounds were identified, which made up 99.06% of the total essential oil content. The major chemical constituents were carvacrol(57.01%), thymol(30.72%), thymol acetate(2.56%), carvacrol acetate(2.14%) and ?-caryophyllene(1.62%). CONCLUSION: The results in our study provides scientific basis for further exploitation and utilization of Mosla chinensis and serves as reference for establishing quality standard of Mosla chinensis herbal medicine.

OBJECTIVE: To provide reference about the way to consummate hospital drug quality management system and ensure drug quality and patients' medication safety.METHODS: Efficient drug maintenance measures were explored by analyzing and summarizing the specific work and conservation procedures of drug maintenance in our hospital which included the humidity of storehouse,monitoring of storage condition,color-code management,expiration date management etc.RESULTS & CONCLUSIONS: Drug maintenance should be aimed at guaranteeing the drug quality;moreover,great importance should be attached to regular quality examination and maintenance of drugs and take timely effective measures aimed at the problems presented so as to ensure the drug quality,safety and effectiveness.

China ; epidemiology ; Humans ; Pneumoconiosis ; epidemiology

Drugs, Chinese Herbal ; therapeutic use ; Humans ; Vascular Diseases ; prevention & control

· AlM:To analyze the association between the macular thickness and emmetropic, low myopic, moderate myopic and highly myopic eyes.
·METHODS:The 276 teenagers (276 eyes) between 18~28 years treated in our hospital from January, 2013 to May, 2014 were selected, whose corrective visual acuity was≥1.0 and intraocular preasure was ≤21mmHg and who were willing to participate in this research.Forty-nine emmetropic, 72 low myopic, 104 moderate myopic and 51 highly myopic eyes were measured by optical coherence tomography ( OCT ) to detect the central subfield thickness, bitamporal, superior, lateral and inferior region thickness of inner and outer region, average thickness of retinal macula, foveal thickness and retinal volume.The thickness of different parts of macula lutea was measured and statistically compared among emmetropic, low myopic, moderate myopic and highly myopic eyes.
· RESULTS: The central subfield thickness of emmetropic, low myopic, moderate myopic and highly myopic eyes were (225.38±20.97), (230.97±19.15), (227.01±16.92), (231.91 ±18.97 )μm. The average thickness of retinal macula, of emmetropic, low myopic, moderate myopic and highly myopic eyes were (280.92±12.71), (278.15± 11.90), (270.05±12.07), (267.93±11.08) μm.There were no significant differences of center thickness (F=1.253, P=0.291) and central subfield thickness ( F=1.034, P=0.378) between emmetropic, low myopic, moderate myopic and highly myopic eyes.The macular thickness of inner and outer region in moderate myopic eyes was significantly less than that in emmetropic eyes, and there was significant difference (P<0.05).Comparison between low myopic, moderate myopic and highly myopic eyes was carried out and macular thickness of all regions of inner and outer region in moderate myopic group was significantly increased, and there was significant difference (P<0.05).There was no significant difference when compared with the macular thickness of inner and outer region of the highly myopic group (P>0.05).
· CONCLUSlON: ln low myopic eyes of teenagers, the center macular thickness do not become thinner. However, the macular thickness of inner and outer region is thinner than that of emmetropic eyes.Furthermore, with the increase of the degree of the myopia, the amount of macular thinner gradually decreases from outer region to inner region.