Objective To understand the psychological experience of patients with malignant tu-mor of oral and maxillofaeial region surgery during early diagnosis and perioperative stage in order to supply reference for clinical staff to carry out psychological support. Methods 12 patients with malignant tumor of oral and maxillofacial region surgery participated in the in- depth interview, the data were analyzed, fin-ished and epurated. Results During the whole process of the early diagnosis and perioperative period,the patients showed negative psychological response, they had to bear significant psychological and financial burden as well as lack of social support. Conclusions Patients with malignant tumor of oral and maxillo-facial region surgery needed humanistic care and emotional support, nursing professionals should provide effective measures to release stress and improve negative emotions, make good use of social resources in or-der to assist them to get through the difficult course.

0.05). Serum glutamate pyruvate transaminase activity significantly decreased in the injured liver BMSCs group (P

AIM:To examine the efficacy and safety of cryotherapy in the management of Fusarium corneal ulcer.
METHODS: Retrospective contract analysis of the clinical data of 41 patients ( 41 eyes ) who infected with Fusarium corneal ulcer. All of them underwent focal lesion keratectomy combined with amniotic membrane transplantation between January 2010 and May 2013. The cryotherapy treatment group of 22 cases (22 eyes), non-cryotherapy group of 19 cases ( 19 eyes ) . All the cases were followed up for 3 ~12mo. We analyzed the healing of corneal ulcer, corneal neovascularization, postoperative visual acuity, complications and ulcer recurrences.
RESULTS: There were no significant difference for the two groups at the cure rate and the recovery rate, the corneal neovascularization and postoperative visual acuity (P>0. 05). The total effective rate and corneal healing time in cryotherapy group was significantly higher than that in non - cryotherapy group ( P < 0. 05 ). The postoperative visual acuity of two group were higher than that of preoperatively ( P < 0. 05 ). There was no complication occurred in cryotherapy group while there was 1 patient of secondary glaucoma in non-cryotherapy group.
CONCLUSION: Cryotherapy is an effective and safe method for the treatment of Fusarium corneal ulcer.

Background The reduced glutathione(GSH) is a water soluble antioxidant in retina and it is an important component of thiol.Diabetes induce the oxidative response of retina.It is very important to detect the content of thiol in retina for the evaluation of its antioxidant role.Objective This study was to investigate the effect of carnosine,aspirin and a combination of carnosine and aspirin eye drops on the change of the thiol contents in the retina of the diabetic rats. Methods Diabetic models were induced in 76 clean male Sprague-Dawley(SD) rats by intraperitoneal injection of streptozotocin(65 mg/kg).The 0.02 mi sodium citrate buffer was injected in 12 control rats.1%camosine eye drops or 0.05%aspirin eye drops were topically administered respectively in carnosine group (19 rats)or aspirin group(19 rats)two times per day for a period of 8 weeks,and the combination of 1%carnosine eye drops and 0.05%aspirin eye drops was used in the combination group at the same way(19 rats).At the end of 8 weeks.The animMs were sacrificed and the eyeballs were enucleated for the detect of thiols in the retina by colorimetry.Results The body weight of SD rats was significantly reduced and the blood glucose level was evidently arose in model group,camosine group and aspirin group in comparison with control group(P＜0.05),but those of combination group compared with control group was insignificantly changed(P＞0.05).The level of thiol in retina was(1 90.35+46.62)ms/gprot in model group,showing a considerable decrease in comparison with control (202.77+53.49 ms/gprot)(P＜0.05).However,the thiol level rose to(378.40±165.06)mg/gprot in camosine group and(351.88±211.62)mg/gprot in aspirin group,showing a statistically significant difference in comparison with control group(P＜0.05).The content of thiol in combination group compared with control group Was insignificant changed(166.972±50.287 mg/gprot us 202.77±53.49 mg/gprot).Curiously the levels in the combination group were not different from the normal control group. Conclusion The carnosine and aspirin eye drops may protect the retina against the oxidative damage caused by diabetes.

Objective To explore the levels of neuron-specific enolase(NSE)in serum and cerebrospinal fluid(CSF) of children with convulsion,and its significance to the diagnosis of neuronal damage was evaluated.Methods Sixty patients were enrolled,and they were divided into 4 groups:non-nervous system disease group,peripheral nervous system disease group,brief convulsion group,and prolonged/status convulsion group.The levels of NSE in serum and CSF were detected in 4 groups,and the electroencephalography(EEG) and computed tomography/magnetic resonance imaging(CT/MRI) examinations were taken to observe the changes in patients with convulsion.Results The levels of NSE in serum and CSF of children with convulsion increased significant,especially in the prolonged/status convulsion group.Changes of NSE levels in serum and CSF were similar.Meanwhile,significant changes were found in EEG between the brief convulsion group and prolonged/status convulsion group;but no significantly changes were found in CT/MRI between the 2 groups.Conclusions NSE detection might be valuable to the diagnosis for neuronal damage in early time.The changes of NSE levels are similar to those in EEG,which are more sensitive than the changes in CT/MRI.It is very important to combine the NSE detection in serum or CSF and EEG examination in early time after convulsion for judgement to the neuronal damage and prognosis.

Aged ; Epiglottis ; pathology ; Female ; Humans ; Laryngeal Neoplasms ; secondary ; Neoplasm Metastasis ; Neoplasm Staging ; Parotid Neoplasms ; pathology

Objective To investigate the features of remodeling of intramyocardial small arteries(IMSAs) in rats with idiopathic hypertension(SHR).Methods12-week-old male SHR rats(experimental group,n=10) with the age and sex matched Wistar Kyoto rats(WKY,control group,n=10) were included in present study.Experiments were continued for 18 weeks.The blood pressure of all the animals was measured at the beginning and at the end of experiment,and then all the animals were sacrificed.The left ventricle tissue sections were stained with HE,Sirius-red,elasticity fibrin and collagen fibrin double staining(P/VB) for morphological evaluation of IMSAs.The wall area(WA),luminal area(LA) and percent wall area(%WA) of IMSAs were assessed with the compute-assisted image analysis system.The vascular smooth muscle cells(VSMCs) were recognized by ?-SMA immunohistochemical staining,the proliferation and apoptosis of IMSAs were demonstrated by proliferating cell nuclear antigen(PCNA) immunohistochemical staining and TdT-mediated dUTP nick-end labeling(TUNEL) technique,respectively.The apoptosis index(AI) and proliferation index(PI) were calculated respectively.Results Systolic blood pressure(SBP) in SHR group at week 12 and week 30 were significantly higher than those in WKY group(172.00?9.78mmHg vs 123.40?5.10mmHg,200.50?14.15mmHg vs 116.30?9.38mmHg,respectively,P

Object To investigate the antagonistic effect of scutellarin on the toxicity of selenium in human liver cells L-02. Methods MTT method was used to observe the effects of scutellarin and selenium on cell growth. The morphological changes of cells were observed by fluorescent microscope. The MDA level, thiol content, and total antioxidative capacity of cells were also measured. Results The optimum condition for scutellarin to promote cell growth was 0.5 mmol/L in 48 h culture period. Comparing with the only selenium treated group, 0.5 mmol/L scutellarin significantly antagonized the inhibition of cell growth caused by 1 and 5 ?mol/L selenium, suppressed the selenium-induced cell damage and the increase of MDA content, as well as increased the thiol content and cell antioxidation capacity. Conclusion Scutellarin markedly antagonizes the toxicity of selenium in human liver cells L-02 by inhibiting lipid peroxidation and promoting cellular antioxidation capacity.

Purpose:The objective of the study is to investigate the pattern of apoptosis and to explore the effect of Fas/FasL on the process of apoptosis induced by daunorubicin (DNR) on human myeloid leukemia cell line HL 60.Methods:The effect on apoptosis and the expression of Fas and FasL antigen induced by DNR on cell line HL 60 were measured by fluorescence microscope ,DNA electrophoresis and flow cytometry analysis.Results:DNR could induce typical apoptosis of HL 60 cells with the DNR plating concentration at 0.1,1 ?g/ml after 6 hours. Morphological changes such as apoptosis bodies, chromatic condensation and cytoplasm budding were observed by fluorescence microscope. Sub G 1 peaks was found by flow cytometry. HL 60 cell apoptosis rate reached a peak at 24 hours. Sub G 1 peaks were not found by flow cytometry with the DNR plating concentration at 10 ?g/ml after 6 hours. DNR could upregulate the expression of Fas and FasL of HL 60 cells.Conclusions:Apoptosis induced by DNR is one of the primary mechanisms in chemotherapy. Fas/FasL system participate in the apoptosis induced by DNR .

Objective To study the characteristics of mesenchymal stem cells(MSCs)derived from chronic myelogenous leukemia(CML)patients' bone marrow and examine their hematopoiesis support in vitro.Methods MSCs from CML were obtained and cultured.Immunophenotype and in vitro differentiation capacities were investigated.Moreover,the Ph chromosome and bcr/abl gene of CML derived MSCs were detected.The expression of cytokines was detected by RT-PCR,and hema- topoiesis support of MSCs in vitro was detected by long-term bone marrow culture and the methylcel- lulose progenitor assay.Results CML derived MSCs showed a typical fibroblast like morphology. They were positive for CD29,CD44 and CD105,while negative for CD14,CD31,CD45,CD34 and HLA-DR.Under suitable conditions,CML derived MSCs could differentiate into osteoblasts and adi- pocytes.Further,CML derived MSCs showed a normal choromosome,and did not express bcr/abl gene.At last,they expressed hematopoiesis cytokines and possessed ability of hematopoiesis support in vitro.Conclusion There are MSCs with multidirectional differentiation potential in CML bone mar- row and they possess the ability of hematopoiesis support.