Bronchial asthma is defined as a chronic inflammatory airway disease in response to a wide variety of provoking stimuli.Over 200 million people in the world are affected with asthma.Because the incidence of asthma appears to be increasing,the importance of proper perioperative management of persons with asthma will also continue to increase.Patients with asthma are thought to be at high risk for pulmonary complications to develop during the perioperative period,and these complications may lead to serious morbidity.So it is very important to control asthma,to prevent and treat pulmonary complications for increasing operational success rate.

Objective To investigate the effect of astilbin on expressions of perforin and granzyme B in activated T cells of mouse heart transplantation model with acute rejection.Methods Cardiomyocytes of BALB/C mouse and spleen cells of C57BL/6 mouse were harvested and made into single cell suspensions.The cardiomyocytes(2?10~5 ml~(-1))as stimulators and spleen cells(1?10~6 ml~(-1)) as responsers were mixed and cultured.The model of mouse heart transplantation with acute rejection in vitro was therefore established.There were two groups in the experiment.Control group is the mixed culture of the cardiomyocytes and spleen cells;Astilbin group is the mixed culture of the cardiomyocytes and spleen cells with astilbin(15?g/ml).Apoptosis of T cells were analyzed by TUNEL assay.The expressions of perforin and granzyme B were measured by RT-PCR.Results Apoptosis of activated T cells in Astilbin group was significantly increased than that of the control group(P