1.Prevention and Management of Overwhelming Postsplenectomy Infection after Traumatic Splenectomy: A Correlative Analysis of 337 Cases
Chenggang JI ; Yanxiang QIAO ; Yueping ZHOU ; Chunxin ZHANG ; Biao LIU ; Jingjun ZENG ; Qinzhi LIU ; Zhimian WU ; Zhaoxu ZHENG
Chinese Journal of Nosocomiology 2006;0(06):-
OBJECTIVE To understand and use reasonably the strategy of prevention and management of overwhelming postsplenectomy infection(OPSI). METHODS According to intervention to patients with postsplenectomy by means of education,vaccination,antibotic prophylaxis after April 1998,clinical and follow-up data were reviewed and analyzed from 337 cases patients with traumatic splenectomy from Jan 1992 to Jan 2004,and correlative factors of four OPSI cases were further analyzed. RESULTS Incidence of OPSI descended obviously after intervention(P
2.Establishment of human IFN-gamma in vitro release assay and its application in tuberculosis diagnosis.
Yingyu CHEN ; Quantao DENG ; Zhihua ZHAN ; Aizhen GUO ; Jie XIANG ; Jun CHEN ; Jinhai ZHOU ; Qinzhi ZENG ; Wu WEI ; Qingwei TONG ; Yanjie CHAO ; Youji KUANG ; Huanchun CHEN
Chinese Journal of Biotechnology 2008;24(9):1653-1657
This study aimed to establish human IFN-gamma (hIFN-gamma) in vitro release assay and to apply it in diagnosis of human tuberculosis. Human IFN-gamma gene was cloned and expressed in Escherichia coli. The recombinant hIFN-gamma was purified and used as immunogen to immunize mice and rabbits respectively. Monoclonal and polyclonal antibodies were respectively developed and a sandwich ELISA was established. The heparized whole blood from 111 active tuberculosis patients and 292 clinical healthy controls were collected. The blood was stimulated with tuberculosis specific fused antigen ESAT-6/CFP-10 and the plasma was collected for IFN-gamma detection. The sensitivity for tuberculosis diagnosis was 95.5%, whereas the positive detection rate for the healthy controls was 16.7%. There was a significant difference between the patients and healthy controls (P<0.01) indicating that this assay had a high sensitivity and specificity, and thus could be promising in tuberculosis diagnosis.
Animals
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Antibodies, Monoclonal
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Enzyme-Linked Immunosorbent Assay
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Female
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Humans
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Interferon-gamma
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immunology
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secretion
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Mice
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Mice, Inbred BALB C
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Mycobacterium tuberculosis
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immunology
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Rabbits
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T-Lymphocytes
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immunology
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Tuberculosis
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diagnosis
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immunology