Objective To explore the application value of endoscopy in the diagnosis and treatment of patients with gastrointestinal bleeding.Methods The clinical data of 74 patients with digestive tract hemorrhage underwent gastroscopy and treatment were retrospectively analyzed.The effect of gastroscopy was compared at different time and different treatment methods.Results Endoscopic examination showed 23 cases of duodenal ulcer disease (31.08％),including 15 cases of gastric ulcer(20.27％),10 cases of acute gastric mucosal lesion (13.51％),8 cases of gastric cancer(10.81％),gastrointestinal complex ulcer in 7 cases (9.46％),4 cases of gastritis (5.41％),anastomotic ulcer or erosion in 4 cases (5.41％),no lesions in 3 cases (4.05 ％).71 cases were clearly diagnosed by gastroscopy,the detection rate was 95.95％,the hemorrhage in 48h positive rate was 100.00％,which was significantly higher than 87.50％ of the hemorrhage 48h detection rate (x2 =13.33,P ＜ 0.05).71 patients received endoscopic treatment,67 patients were successful,the success rate was 94.37％,the injection of hemostatic success in 16 cases (94.12％),spraying hemostasis successfully in 51 cases (94.44％),there was no significant difference (x2 =-0.01,P ＞ 0.05).Conclusion In the patients with gastrointestinal bleeding,gastroscopy has advantages of high diagnostic accuracy,accurate curative effect,can shorten the bleeding time,improve the success rate of hemostasis,improve the prognosis,with high clinical value.