Objective: To investigate the effects of insulin on the DNA synthesis and cell cycle distribution of human periodontal ligament cells (PDLs). Methods:PDLs were treated with insulin at the doses (U/L) of 0.01～1 000 for 72 h,DNA synthesis of the cells was measured by 3H-TdR incorporation and cell cycle distribution was studied by flow cytometer. Results : Insulin at 1 U/L to 1 000 U/L showed a significant concentration dependent stimulation of 3H thymidine uptake, and the effect of insulin at 100 ～ 1 000 U/L were not statistically different from that at 10 U/L. The percentage of PDL cells in G1 phase during exponential growth was decreased by insulin, while that in S phase and (G2+M) was increased in experimental group. Conclusion: Insulin increases DNA synthesis of PDL cells ,and stimulates the transformation of cells from G1 to S phase.

Objective: To evaluate the effects of guided tissue regeneration(GTR) in the treatment of degree Ⅱ-Ⅲ furcation defects with collagen membranes.Methods: 11 sites with Ⅱ-Ⅲ furcation defects were treated with collagen membranes(group of GTR); 10 sites received flap operation(group of FO). Clinical indexes, radiographs and gingival crevicular fluid(GCF) was collected before surgery and 3 months after treatment. Alkaline phosphatase(ALP) in GCF was measured. The data were analyzed statistically. Results: 3 months after surgery, the clinical parameters of PD, PFD and AL were decreased(P0.05). The decreace of the clinical parameters measured in group GTR was more than that in group FO(P

0.05), on the 4th day 7.56?6.87 and 10.00?7.07 (P0.05). After therapy all the data of electrocardiogram, blood routine examination and blood biochemical test of the cases were without clinical significance. Conclusion:50 g/L amlexanox is effective and safe in the treatment of RAU.

Epithelium ; Gingiva ; immunology ; Humans ; Periodontitis ; immunology

Objective:To study the effect of dipeptidyl peptidase I(DPPI)on the damage of alveolar bone and inflammatory re-sponse of periodontium in periodontitis mice.Methods:Periodontitis model of left first molars was established in 1 0 DPPI-/-mice and 1 0 wild type(WT)mice by E.coli LPS injection and the right ones were served as the control by PBS injection.Micro-CT scanning and HE staining were performed to compare the damage of alveolar bone and inflammatory response of periodontium between the 2 groups.Results:The decreace of alveolar bone height in DPPI-/-mice was less than that in WT mice〔(Maxillary:(0.001 7 ± 0.000 4)mm vs (0.202 0 ±0.008 6)mm;Mandibular:(0.034 2 ±0.002 9)mm vs (0.332 8 ±0.01 2 5)mm〕(P<0.05).HE staining showed that the inflammatory response of periodontium in DPPI-/-mice was less severe than that in WT mice.Conclusion:DPPI may promote the inflammatory response of periodontis in mice.

100 patients with recurrent aphthous ulcer (RAU) were randomly divided into two groups, 50 in each group. The patients in test group were treated with Xipayi mouthwash, those in control were given Chlorhexidine mouthwash. All the patients were treated for 7 days. The efficacy of Xipayi mouthwash is superior to Chlorhexidine in the treatment of RAU.

Objective: To investigate the effect of insulin-like growth factor-Ⅰon osteocalcin secretion in periodontal ligament cells. Methods: Human periodontal ligament cells were cultured by tissue explant in vitro, and the concentration of osteocalcin were determined with radio-immunological method. Results: Under the condition of L-ascorbic acid and β-glycerophosphate in culture medium, human periodontal ligament cells secreted osteocalcin time-dependently and peaked at the third week; IGF-Ⅰ3.125 ng/ml，6.250 ng/ml, 12.500 ng/ml, 25.000 ng/ml could promote the secretion of osteocalcin dose dependently. Conclusion: IGF-Ⅰ can increase the secretion of osteocalcin in human periodontal ligament cells.

Objective:To evaluate the biocompatibility of insulin-like growth factor I (IGF-1) gene transfected bone marrow stem cells (MSCs) with ostrich true bone ceramic (OTBC). Methods:Rat MSCs were transfected with IGF-1 gene, and positive clones were selected by G418. The expression of IGF-1 protein in the MSCs was detected by immunocytochemical technique. The IGF-1 transfected MSCs were cultured with OTBC and the morphology of the cells was observed by scanning electronic microscope(SEM) at different time point. Results:Immunohistochemical staining suggested that the IGF-1 protein was expressed in the IGF-1 transfected MSCs. The cells adhered to OTBC and stretched well after 24 h of culture. The IGF-1 transfected MSCs proliferated on the surface of OTBC with culture time.Conclusion:The OTBC has a good biocompatibility with IGF-1 transfected MSCs.

0.05)between DA and DE when the diameter of gel microspheres was20?m～40?m.CONCLUSI_ ON:The drug-loading pattern of BMP 2 -DEX-GMA gel microspheres can be influenced by its particle size,but its drug release character can not be changed,the controlled release of drugs can be achieved through changing the nature of its drug-loading material.

Objective:To study factors influencing postoperative pancreatic fistula rates with a view to prevent postoperation pancreatic fistula from happening.Methods:This is a retrospective study on 281 patients who underwent distal pancreatectomy at the First Affiliated Hospital of China Medical University from March 2011 to April 2018. There were 89 males and 192 females, with the age of (51.01±13.65) years. Univariate and multivariate logistic regression analyses were used to analyze the following factors on the occurrence of pancreatic fistula after operation: gender, age, body mass index(BMI), tumor characteristics, preoperative fasting blood glucose, blood biochemistry, liver function and surgical indications.Results:Of the 281 patients who underwent distal pancreatectomy in this study, 245 (87.2%) did not develop pancreatic fistula / biochemical leakage, while 36(12.8%) patients developed clinically significant pancreatic fistula (B/C grade). Univariate analysis showed the factors which affected the incidence of pancreatic fistula after surgery to include: BMI, preoperative fasting blood glucose, and whether the main pancreatic duct was ligated (all P<0.05). Multivariate logistic regression analysis showed that the independent factors affecting pancreatic fistula incidence after surgery were BMI≥25 kg/m 2 ( OR=2.354, 95% CI: 1.137-4.873, P<0.05), and main pancreatic duct was not ligated ( OR=4.067, 95% CI: 1.191-13.885, P<0.05). Conclusions:A high BMI increased the risk of postoperative pancreatic fistula, while ligation of main pancreatic duct during surgery reduced the risk.