Ginsenosides in the decoction of Radix Ginseng, Radix Ginseng with Flos Lonicerae, Radix Polygoni Multiflori or Radix Astragali have been investigated by high performance liquid chromatography (HPLC) and electrospray ionization mass spectrometric method (ESI-MS). Change of the content of ginsenosides was nonlinear in diverse combinative proportion of Radix Ginseng with Flos Lonicerae, while the stripping of ginsenosides was promoted by a small amount of Radix Polygoni Multiflori. In the combinative decoction of Radix Ginseng with Radix Astragali, ginsenosides contents were increased compared to single decoction of Radix Ginseng. Besides, ferric reducing antioxidant power (FRAP) method was developed for determination of the total antioxidative activity of n-butanol and water-soluble extracts from the decoction. The experimental results showed that antioxidative activity was better in the combinative decoction than that in single decoction, and the FRAP values of n-butanol extract were also greater compared with that of water extract.

Objective To explore the relationship between the level of plasma ADAMTS4 and the vulnerability of carotid atherosclerotic plaque in patients with carotid artery stenosis and the clinical value of evaluating the vulnerability of the carotid atherosclerotic plaque.Methods 60 patients with carotid artery stenosis were divided into stable plaque group and vulnerable plaque group according to the histopathological typing after carotid endarterectomy (CEA).30 patients who had atherosclerosis but no carotid plaque formation served as control.Using ELISA to measure the level of plasma ADAMTS4.Results The level of plasma ADAMTS4 was significantly higher in the vulnerable-plaque group (112.74 ± 17.47) ng/ml compared with the stable plaque group (56.67 ± 22.14)ng/ml and the control group (56.67 ± 22.14)ng/ ml,P ＜ 0.001.Logistic analysis showed that high level of plasma ADAMTS4 was risk factor for vulnerability of carotid atherosclerotic plaque (P ＜ 0.05,OR =8.240,95 ％ CI:1.512-22.915).ROC curve showed that ADAMTS4 =100.935 ng/ml could accurately predict significant vulnerability with accuracy of 94.3％.Conclusion ADAMTS4 is an effective,sensitive and non-invasive biomarker to evaluate the vulnerability of carotid atherosclerotic plaque.

Objective:The mechanical properties of costal cartilage of different genders and layers were tested, and the differences in mechanical properties of costal cartilage of different genders and layers were discussed. The theoretical basis was provided for the clinical use of costal cartilage of different layers in different situations, and the long-term effect after cartilage implantation could be predicted.Methods:According to the inclusion criteria, from June 2018 to December 2019, patients who receive dauricle reconstruction with costal cartilage or supporting treatment with costal cartilage were collected from the Inpatient and Outpatient Department of Plastic Surgery of Sichuan Provincial People’s Hospital. The remaining central and marginal layers of the seventh costal cartilage were made into different sizes of cartilage blocks. The tensile strength test and compression test (including creep test and stress relaxation test) were carried out respectively. The counting data were described by the number of cases, and Fisher exact test was used to analyze the differences between groups; the measurement data were expressed by mean ± standard deviation, and the comparison between groups was analyzed witht-test.Results:A total of 28 patients, including 16 males, aged 12-24 years old and 12 females, aged 12-18 years old, were collected. In the tensile strength test, the tensile fracture rate of the central layer of costal cartilage in male was 87.5% (14 / 16) and that of marginal layer was 0, the difference was statistically significant ( P< 0.001). The tensile fracture rate of central layer of costal cartilage in female was 83.3% (10 / 12), and that of marginal layer was 0, the difference was statistically significant ( P< 0.001). In the creep test, there was no significant difference between the central layer and the marginal layer between 0.5 min and 2 min in men ( t=-1.439, P= 0.171), and there was no significant difference between the central layer and the marginal layer in women ( t=-0.731, P=0.480). In the stress relaxation test, there was no significant difference between the central layer of costal cartilage and the marginal layer in male ( t=-2.053, P=0.058); in female, the stress relaxation in the central layer of costal cartilage was (0.006 ± 0.003)%, and that in the marginal layer was (0.011 ± 0.004)%, which was significantly higher than that in the central layer ( t=-3.342, P=0.007). Conclusions:The central layer of the costal cartilage in young people is worse than the marginal layer in tensile strength, and the calcification obviously weakens the tensile strength of the costal cartilage. Clinically, considering the calcification of the cartilage, the appropriate layer should be selected according to different parts and uses.

Objective:The mechanical properties of costal cartilage of different genders and layers were tested, and the differences in mechanical properties of costal cartilage of different genders and layers were discussed. The theoretical basis was provided for the clinical use of costal cartilage of different layers in different situations, and the long-term effect after cartilage implantation could be predicted.Methods:According to the inclusion criteria, from June 2018 to December 2019, patients who receive dauricle reconstruction with costal cartilage or supporting treatment with costal cartilage were collected from the Inpatient and Outpatient Department of Plastic Surgery of Sichuan Provincial People’s Hospital. The remaining central and marginal layers of the seventh costal cartilage were made into different sizes of cartilage blocks. The tensile strength test and compression test (including creep test and stress relaxation test) were carried out respectively. The counting data were described by the number of cases, and Fisher exact test was used to analyze the differences between groups; the measurement data were expressed by mean ± standard deviation, and the comparison between groups was analyzed witht-test.Results:A total of 28 patients, including 16 males, aged 12-24 years old and 12 females, aged 12-18 years old, were collected. In the tensile strength test, the tensile fracture rate of the central layer of costal cartilage in male was 87.5% (14 / 16) and that of marginal layer was 0, the difference was statistically significant ( P< 0.001). The tensile fracture rate of central layer of costal cartilage in female was 83.3% (10 / 12), and that of marginal layer was 0, the difference was statistically significant ( P< 0.001). In the creep test, there was no significant difference between the central layer and the marginal layer between 0.5 min and 2 min in men ( t=-1.439, P= 0.171), and there was no significant difference between the central layer and the marginal layer in women ( t=-0.731, P=0.480). In the stress relaxation test, there was no significant difference between the central layer of costal cartilage and the marginal layer in male ( t=-2.053, P=0.058); in female, the stress relaxation in the central layer of costal cartilage was (0.006 ± 0.003)%, and that in the marginal layer was (0.011 ± 0.004)%, which was significantly higher than that in the central layer ( t=-3.342, P=0.007). Conclusions:The central layer of the costal cartilage in young people is worse than the marginal layer in tensile strength, and the calcification obviously weakens the tensile strength of the costal cartilage. Clinically, considering the calcification of the cartilage, the appropriate layer should be selected according to different parts and uses.

Objective:A mouse model of pancreatitis induced by coxsackievirus B3 (CVB3) was established. The pathological change of pancreas and the infiltration of Th17/Treg cells were observed.Methods:The BALB/c mice were inoculated intraperitoneally with CVB3 to induce acute viral pancreatitis model. Then the pathological changes of pancreas were observed by HE staining; the viral RNA load and relative expression of cytokines (IFN-γ, IL-6 and IL-17) mRNA were detected by q-PCR; the proportion of infiltrated CD45 + CD3 + T cells, CD4 + and CD8 + T cells, Th17 and Treg cells in the pancreas was determined by flow cytometry. Results:Three days after CVB3 infection, the viral RNA load in pancreas was the highest (0.96±0.18) and gradually decreased with prolongation of infection. Compared with the 3 dpi group, the viral RNA load in pancreas was decreased (0.96±0.18 vs. 0.62±0.14) at 7 dpi, but there was no statistically significant difference. In addition, the infiltration of immune cell in pancreas increased significantly after 7dpi and the pathological score >2. The percent of infiltrated Th17 cells (1.05±0.21 vs. 22.13±5.79) and Treg cells (3.11±0.78 vs. 8.25±1.30) among CD4 + T cells significantly increased after infection (P<0.05), and the Th17/Treg also increased (P<0.01). Compared with the control group, the relative mRNA expression of IFN-γ (1.05±0.23 vs. 672.6±47.67), IL-6 (1.00±0.38 vs. 68.28±4.57), and IL-17 (1.01±0.11 vs. 54.15±7.94) in pancreas increased at 7 days after CVB3 infection ( P<0.01). Conclusions:The infiltration of Th17/Treg cells and the expression of related cytokines related cytokines IL-6 and IL-17 mRNA were upregulated in pancreas, which promoted the process of CVB3-induced pancreatitis.

Objective:To analyze the association of respiratory syncytial virus infection with meteorological factors and to predict and explain the trends.Methods:Data of cases with severe acute respiratory infections in hospitalized children in Xuzhou City were collected from 2015-2021. Respiratory syncytial virus (RSV) was detected by real-time fluorescence polymerase chain reaction. The result were statistically analyzed using SPSS 26.0 software, including constructing a negative binomial regression model to explore meteorological factors that impact RSV detection and a multivariate time series model to predict its epidemiological trend from 2020 to 2021.Results:A total of 1 663 samples of children with severe acute respiratory infections were collected from 2015to 2021, of which 218 (13.1%) were positive for RSV. Seasonal effects on RSV detection were evident: there was a 1-year cycle with a peak in winter (December-February) and a trough in summer (June-August). The negative binomial regression analysis showed that monthly mean temperature, monthly mean relative humidity, and monthly total sunshine hours may influenced RSV detection. The prediction result of the time series model with sunshine hours as the covariate showed that the prediction was better for 2020, and the actual values were close to the predicted values. The expected trends in 2021 were consistent, but the actual values were higher than predicted.Conclusions:Monthly mean temperature, monthly mean relative humidity, and monthly total sunshine hours may influence RSV detection in the Xuzhou region.A prediction model can be built using data from 2015-2019, where deviations in the predicted values for 2021, reflecting that disease prevalence is multifactorial correlated, suggest a possible rise in RSV prevalence in the future.