OBJECTIVETo clone and sequence the upstream of mouse dentin sialophosphoprotein promoter.

METHODSGenomic DNA was obtained from Balb/c mouse blood. The upstream of mouse dentin sialophosphoprotein promoter segments was obtained by PCR. Then the segments were inserted into T-vector. The plasmids were identified by digestion with the restriction enzyme analysis. The positive clone was sequenced and compared with Genebank.

RESULTSThe upstream of mouse dentin sialophosphoprotein promoter was divided into three sequences and three different target segments with 997 bp, 1004 bp and 674 bp in length were obtained. After identified, sequenced and compared with Genebank, the sequences of the segments were consistent with those displayed on Genebank by 99%.

CONCLUSIONThe clone of the upstream of mouse dentin sialophosphoprotein promoter was successful. This work will help to study the regulation of DSPP expression.

Animals ; Cloning, Molecular ; Extracellular Matrix Proteins ; genetics ; Mice ; Mice, Inbred BALB C ; Phosphoproteins ; genetics ; Promoter Regions, Genetic ; Sequence Analysis, DNA ; Sialoglycoproteins ; genetics