Proteomics is an important high throughout method in modern life science.In this paper,the definition,background and methods used in proteomics were introduced,and the last part was focused on its application in parasitology.

0 05), being ( 187 0?10 7) nmol/L in average. Moreo ver, it was not significantly different from the cells cultured at day 0, ei ther . However, [Ca 2+ ]i increased significantly(P

Objective To understand the immigrants' recognition of schistosomiasis and their relevant behaviors in the South-North Water-Diversion Middle-line Project areas in Hubei Province.Methods The investigation objects were selected by the method of stratified sampling among immigrants in the project areas and were surveyed through oral interview and questionnaire.Results A total of 1 010 immigrants were investigated and 1 005 questionnaires were indentified as effective ones.The awareness rates of schistosomiasis and the correct rates of related behaviors among the immigrants were still not satisfying.Conclusions The immigrants' recognition of schistosomiasis and their relevant behaviors in South-North Water-Diversion Middle-line Project areas in Hubei Province still need to be improved through health education and other measures.

OBJECTIVETo examine the differential expression of miRNAs in colon cancer tissues and matched tumor adjacent tissues.

METHODSDifferential expression of microRNAs in twenty paired human colon cancer tissues and adjacent tissues were detected by QPCR. miRNAs with significantly differential expression (fold change >2.4 and P<0.01) were screened to analyze their accumulation by Cluster analysis. Thus correlation of miRNA and other colon cancer-associated proteins was examined.

RESULTSExpressions of 17 miRNAs were significantly reduced in colon cancer tissues. Cluster analysis showed that miR763-3, miR451 and miR99a had similar expression. Plasma CK20 level was negatively correlated with miR100 (r=-0.948), miR152a-5p (r=-0.948), miR125b (r=-0.949), miR145 (r=-0.949) and miR145*(r=-0.949) (all P<0.05).

CONCLUSIONmiR145 and other 16 miRNAs may be used as diagnostic molecular markers of colon cancer, and miR100, miR125a-5p, miR125b, miR145 and miR145* may become the molecular markers of colon cancer lymph node metastasis.

Cluster Analysis ; Colonic Neoplasms ; genetics ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Genomics ; Humans ; Lymphatic Metastasis ; MicroRNAs ; genetics

This study was aimed to analyze the value of the SPECT MPI to myocardial ischemia and cardiac function parameters in the CAD prognosis assessment. A total of 890 patients with suspected CAD underwent adenosine loading stress gated SPECT. At a mean follow-up of (2.3 +/- 0. 4) year, a total of 37 adverse events occurred, including 12 cardiac deaths and 25 nonfatal myocardial infarctions. Univariate Cox analysis showed that diabetes (wald 6.95, P < 0.01), SSS (wald 24.31, P < 0.001), EF (wald 17.14, P < 0.001), ESV (wald 8.58, P < 0.01) and EDV (wald 7.95, P < 0.01) were significant predictors of MACEs. Multivariate Cox analysis showed that SSS (wald 6.69, P < 0.05) and EF (wald 4.70, P < 0.05) were independent predictors. According to the results, SSS and EF are both independent predictors of MACEs.
Adenosine ; Aged ; Cardiac-Gated Single-Photon Emission Computer-Assisted Tomography ; methods ; Coronary Disease ; diagnostic imaging ; physiopathology ; Female ; Humans ; Male ; Middle Aged ; Prognosis ; Tomography, Emission-Computed, Single-Photon ; methods ; Ventricular Function, Left ; physiology