To investigate the distribution of virulence associated factors of 5 strains of Streptococcus suis type 2 from slaughtered pigs isolated in Fujian province , 7 virulence genes were detected through PCR amplification. They were the glutamate dehydrogenase gene(gdh), the capsular polysaccharides gene(cps), the extracellular factor gene(ef), the muramidate-released protein gene(mrp), the suilysin gene(sly), the fibronectin-binding protein gene(fbps) and the virulence-associated sequence or f2. Among these 5 strains isolated, the virulence genotypes of 4 strains S.suis type 2 and strain SS2PFJ07 was gdh+/cps2J+/mrp+/ef-/sly-/fbps+/orf2+ and the other one strain isolated from slaughtered pigs was gdh+/cps2J+/mrp+/ef+/sly+/fbps+ /orf2+ respectively. This results showed that there are at least 2 virulence genotypes in Streptococcus suis type 2 from slaughtered pigs isolated in Fujian province.

Objective:To analyze the risk factors of pelvic infection after gynecological malignant tumor surgery and construct a predictive model.Methods:The convenient sampling method was used to collect the clinical data of 278 patients who underwent gynecological malignant tumor surgery in Shanxi Provincial People's Hospital from June 2018 to June 2020. According to the occurrence of postoperative pelvic infection, the patients were divided into the infection group ( n=29) and the non-infection group ( n=249) . Univariate and binomial Logistic regression analysis was used to investigate the risk factors of pelvic infection after gynecological malignant tumor surgery, and ROC curve was used to analyze the predictive value of the prediction model. Results:The incidence of pelvic infection in 278 patients with gynecological malignancies after surgery was 10.43% (29/278) . Binary logistic regression analysis showed that whether there were diabetes, chronic pelvic pain, history of preoperative pelvic infection and operation method, operation duration, and hospital stay were the influencing factors of pelvic infection after gynecological malignant tumor surgery ( P<0.05) . ROC curve analysis showed that diabetes mellitus, chronic pelvic pain, history of preoperative pelvic infection, surgical method, surgical duration and length of hospital stay all had certain predictive value for pelvic infection after gynecological malignant tumor surgery, while the predictive value of combined application was higher than that of each index alone. The area under the ROC curve was 0.821 (95% CI: 0.729-0.915) , and the accuracy was 0.817. Conclusions:The presence of diabetes mellitus, chronic pelvic pain, preoperative history of pelvic infection, surgical method, duration of surgery and length of hospital stay are the influential factors for pelvic infection after gynecological malignant tumor surgery. The combined prediction model established based on the above factors has good prediction ability.

Objective To evaluate and compare the subjective and objective visual quality of YAG laser posterior capsulotomy.Methods A series of case observation study was designed.Twenty-eight patients (38 eyes)underwent Nd:YAG laser posterior capsulotomy in the Eye Hospital of Wenzhou Medical University were retrospectively collected from 2015 to 2016.Patients were divided into circular capsulotomy group (20 patients 23 eyes) and cruciate capsulotomy group (12 patients 15 eyes) based on the capsulotomy technique (round incision of posterior capsule or cross incision of posterior capsule).The uncorrected visual acuity (UCVA) and best corrected visual acuity (BCVA) were examined before and 2 weeks after operation,the intraocular pressure was also measured before operation,1 hour after operation and 2 weeks after operation.The Strehl ratio (SR),modulation transfer function (MTF) cut-off,objective scatter index (OSI),OQAS values (OVs) were analyzed by Two-channel visual quality analysis system OQAS Ⅱ at 1 h and 2 weeks after operation.The changes of intraocular scattering and visual quality at 1 h and 2 weeks after operation with different posterior capsulotomy methods were compared and analyzed.Results At 2 weeks after operation,the UCVA was 0.18±0.15 and 0.16±0.14,and the BCVA was 0.10±0.10 and 0.10±0.11,respectively in the circular capsulotomy group and cruciate capsulotomy group,which was higher than those before operation (all at P＜0.05),but there was no significant difference in UCVA and BCVA between the two groups (all at P＞0.05).There was no significant difference in intraocular pressure between the two groups at 1 hour and 2 weeks after operation (both at P＞0.05).In the circular capsulotomy group,the incidence of black shadow drift was 39.1％ and 30.4％ respectively at 1 hour and 2 weeks after operation,which was higher than that in the cruciate capsulotomy group (6.7％ and 6.7 ％),with no significant differences between the two groups (P =0.065,0.177).No significant difference were found in the incidence of black shadow drift before eyes between the two groups (P＞0.05),and there was no significant differences in the OQAS parameters OSI,MTF,SR,OV100％,OV20％ and OV9％ between the two groups (all at P＞0.05).Conclusions After Nd:YAG laser capsulotomy,the incidence of black shadow drift in the cruciate capsulotomy group is lower than that in the circular capsulotomy group.There is no significant difference in the visual acuity,intraocular pressure,intraocular scattering and visual quality parameters between circular posterior capsulotomy and cross posterior capsulotomy.