1.RYBP activates PARP-1 induced Parthanatos in esophageal squamous cell carcinoma cells and enhances response to YM155
Yue KE ; Yuxing LI ; Xiaobo SHI ; Wei GUO ; Xiaoxiao LIU ; Yuchen WANG ; Qinli RUAN ; Jiyuan PAN ; Xiaoping YANG
Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(2):233-237,256
【Objective】 To explore the role of RYBP in activating PARP-1 dependent Parthanatos and promoting response to YM155 in esophageal squamous cell carcinoma. 【Methods】 CCK-8 and flow cytometry were used to analyze the inhibition ratio and cell death percentage after YM155 treatment in both RYBP overexpression group and control group. Western blotting was used to detect the expression of Parthanatos-related proteins. 【Results】 Compared with control group, RYBP overexpression group showed higher inhibition ratio and cell death percentage after YM155 treatment. Overexpression of RYBP activated PARP-1 with or without YM155 treatment. Besides, after YM155 treatment, KYSE170-RYBP showed more PAR accumulation in the nucleus, AIF translocation from mitochondria to the nucleus than control cells. 【Conclusion】 RYBP can activate PARP-1/PAR/AIF-dependent induced Parthanatos in esophageal squamous cell carcinoma and enhance response to YM155.
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