Background:There is growing evidence that cytokines are the key elements in the initiation,evolution,and,ultimately,the resolution of inflammatory bowel disease (IBD).Ahns:To investigate the differences of cytokine expressions in intestinal mucosa between IBD patients and normal controls.Methods:Thirty cases of colonoscopic or enteroscopic biopsied mucosal tissue were collected from 7 normal subjects,12 ulcerative colitis (UC),and 11 definite or suspicious Crohn's disease (CD) during Jan.2014 to Dec.2014 at Shanghai East Hospital South Branch.Using the suspension chip technology,expressions of 27 cytokines in mucosal tissues were detected.Results:Most of the cytokines in intestinal mucosa of patients with IBD were decreased as compared with the normal controls,whereas the expressions of interleukin-5 (IL-5) and IL-7 were increased.Among the 27 cytokines,statistically significant differences were seen in expressions of IL-1β,IL-2,IL-15,interferon-γ-induced protein-10 (IP-10),monocyte chemoattractant protein-1 (MCP-1),macrophage inflammatory protein-1α (MIP-1α) and MIP-1β between UC,CD and normal controls (P ＜ 0.05);in addition,significant differences were seen in expressions of IL-8 and platelet-derived growth factor-BB (PDGF-BB) between CD and normal controls (P ＜ 0.05).No significant differences were observed in expressions of all 27 cytokines between UC and CD (P ＞ 0.05),as well as between active and remission stages of IBD (P ＞ 0.05).Conclusions:Expressions of IL-1β,IL-2,IL-15,IP-10,MCP-1,MIP-1α and MIP-1β are significantly down-regulated in intestinal mucosa of IBD patients.It may help to further explore the role of cytokines in IBD.