AIM: Based on the water-solubility of polyethylene glycol (PEG) with different relative molecular weight (RMW) and sodium alginate, we investigated the porous structure and property of calcium alginate scaffold with different RMW and dosages of PEG, which was used as porogenic agent. METHODS: The experiment was carried out in the Key Laboratory of Biomedical Engineering and Biomaterials, Jinan University from March 2006 to September 2007.①PEG at different RMWs (Mw=2 000, 4 000, 6 000, 8 000, 10 000, 20 000, 35 000) and different dosages (mass fraction=0, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06) were added into sodium alginate solution at 0.02 mass fraction. Then alginate was crosslinked, solidified and molded into indiscerptible film and graininess through Ca2+. PEG was dissolved by water. Therefore, a mass of porous structures could be formed in alginate.②Water content and swelling ratio of alginate was tested. Scanning electron microscope was used to observe the porous configuration. RESULTS: ①Porous alginate scaffold could be obtained through PEG and it exhibited good intensity and toughness, with water content reaching 92%. The scaffold could be formed into film and graininess.②Scanning electron microscope results revealed that the distribution of porous structure was uniformed. The aperture was 43.75 ?m-2.8 mm, and could be controlled by PEG's RMW and dosage. Inflated and uniform aperture structure was harvested when RMW of PEG was 4 000 and 6 000. CONCLUSION: Porous alginate structure can be obtained through regulating PEG's RMW and dosage. High water content and porosity of alginate scaffold material can be used in cell culture of tissue engineering and used as controlled release matrix of bio-active component.

Objective To fabricate a novel polyvinyl alcohol (PVA)-chitosan(Cs)-collagen (Col) composite material and to confirm the feasibility of its application as a scaffold in tissue engineering.Methods PVA was blended with chitosan and collagen.The water content,swelling ratio,and tensile strength of the scaffolds were tested.SEM was used to observe the histological modality of the cross section.Results Scaffolds which al'e composite with different molecular weight and different amount of Cs and Collagen ale made,with water content ranging from 60.15% to 72.50% and swelling ratio being from 185.33% to 317.57%.The tensile strength of the composite material is 5.70MPa.The inner modality and structure of the scaffolds varied as the proportion of the chemical components changed.Conclusion PVA-Cs-Col scaffolds have high water content and proper swelling ratio and ale rich in porous structure.When the blending proportion is Cs:PVA:COI=30:15:0.20,the scaffold performs best,which shows to be a suitable structure for tissue engineering scaffold.

Objective:To establish an HPLC method to determine the content of rosmarinic acid in Wuling capsules. Methods:The chromatographic column was Linksil-ODS (250 mm × 4. 6 mm,5 μm) with a gradient elution mode at the flow rate of 0. 8 ml· min-1,and the mobile phase was acetonitrile-water (32∶68) including 0. 5% acetic acid. The detection wavelength was 319 nm, the column temperature was 25 ℃ and the injection volume was 20 μl. Results: The linear range was 2-80 μg·ml-1 and the standard curve showed good linear regression within the range(r=0. 999 8). And the average recovery was 97. 07﹪ (n=6) with the inter-day precision (RSD) of 1. 35 %. Conclusion:The method is accurate and rapid with good reproducibility in the determination of ros-marinic acid in Wuling capsules, which can be used to control the quality of rosmarinic acid in Wuling capsules.

The qualitative and quantitative analysis of active constituents in Fructus Psoraleae is presented by high-performance liquid chromatography (HPLC) coupled with different detections.Extracts of Fructus Psoraleae were examined by HPLC with ion trap mass spectrometry (IT-MS) and 18 major compounds of coumarins,benzofuran glycosides,flavonoids,and meroterpene were identified.The determination of four major constituents including bavachin,isobavachalcone,bavachinin,and bakuchiol was accomplished by HPLC with UV,MS,and electrochemical detection (ECD).These methods were evaluated for a number of validation characteristics (repeatability,LOD,calibration range,and recovery).ECD obtained a high sensitivity for analysis of the four components; MS provided a high selectivity and sensitivity for determination of bavachin,isobavachalcone,and bavachinin in negative-ion mode.After optimization of the methods,separation,identification.and quantification of the four components in Fructus Psoraleae were comprehensively tested by HPLC with UV,MS,and ECD.

Objective To explore the clinical manifestation,laboratory tests,imaging and treatment of general paralysis of insane(GPI).Methods The clinical data of 2 patients with GPI analyzed retrospectively.Results 2 sufferers were both male,and mainly presented with memory decline and mental disorder.One case presented with visceral crisis.Both rapid plasma reagin test and treponema gelatin agglutination test in serum and CSF were positive.Brain MRI scanning showed ventriculomegaly.After antisyphilitic treatments,2 patients obviously improved.Conclusion GPI are often seen in middle-aged males and its misdiagnosis rate is high.The diagnosis of GPI should be considered in young patients with dementia without clear reason.Early diagnosis and active treatments are important for the prognosis of this disease.

OBJECTIVE:To establish a HPLC method for the determination of yohimbine in corynanthe yohimbe K.schum.METHODS:The analysis was performed on C18 column with column under room temperature.The mobile phase consisted of methanol-phosphoric acid buffer-triethylamine(30∶ 70∶ 0.5,pH5.0)with a flow rate of 1.0mL? min-1.The wavelength was set at 270nm.RESULTS:There was a good linear relationship when the sample size of yohimbine was over a range of 10～ 1 000? g? mL-1(r=0.999 8),with recovery at 96.5% and precision at 1.23%.CONCLUSION:This method was simple and reliable,and suitable for the determination of content of yohimbine.

BACKGROUND: Polyvinyl alcohol (PVA) has similar water content as human tissues, good biocompatibility and high mechanical strength, thereby being utilized as tissue-engineered matrix material. However, the modification in cellular affinity benefits the function as tissue regeneration scaffold materials. OBJECTIVE: To prepare chitosan (CS) / PVA composite scaffold and explore the feasibility of serving as a scaffold for tissue engineering. METHODS: Scaffolds of CS/PVA was fabricated by blending PVA-124 with various amounts of CS using film forming, particle forming, and free-drying methods. Water content, light transmittance, and swelling ratio were tested. Scanning electron microscope was used to observe the configuration of the cress section. RESULTS AND CONCLUSION: A total of 7 different proportional scaffolds were obtained with different PVA and CS dosage. The composite film showed light transmittance between 70%-80%, high water content between 121.2%-162.5% and swelling ratio between 60.3%-133.7%.Composita particle and sponge obtained water content between 82.0%-461.2% and 280.8%-1 939.0%. Composite scaffold with the amount ratio of PVA 0.75 g/CS 0.15 g exhibited the best performance. Scanning electron microscope showed that at a mass ratio of PVA 0.75 g/CS 0.15 g, the scaffold prepared by freeze-drying method had regular, tluffy inner structure, with good mechanics and high water content.

BACKGROUND:Polyvinyl alcohol(PVA)has similar water content as human tissues,good biocompatibility and high mechanical strength,thereby being utilized as tissue-engineered matrix material.However,the modification in cellular affinity benefits the function as tissue regeneration scaffold materials.OBJECTIVE:To prepare chitosan(CS)/PVA composite scaffold and explore the feasibility of serving as a scaffold for tissue engineering.METHODS:Scaffolds of CS/PVA was fabricated by blending PVA-124 with various amounts of CS using film forming,particle forming,and free-drying methods.Water content,light transmittance,and swelling ratio were tested.Scanning electron microscope was used to observe the configuration of the cross section.RESULTS AND CONCLUSION:A total of 7 different proportional scaffolds were obtained with different PVA and CS dosage.The composite film showed light transmittance between 70%-80%,high water content between 121.2%-162.5% and swelling ratio between 60.3%-133.7%.Composite particle and sponge obtained water content between 82.0%-461.2% and 280.8%-1 939.0%.Composite scaffold with the amount ratio of PVA 0.75 g/CS 0.15 g exhibited the best performance.Scanning electron microscope showed that at a mass ratio of PVA 0.75 g/CS 0.15 g,the scaffold prepared by freeze-drying method had regular,fluffy inner structure,with good mechanics and high water content.

Objective:To establish a method for determining osthole in Baicao Fuyanqing suppositories by HPLC. Methods:The de-termination was carried out on a DL-Cl8column (4.6 mm ×150 mm, 5 μm). The mobile phase consisted of acetonitrile-water (65 ∶35) with the detection wavelength at 321nm and the flow rate of 1. 00 ml·min-1 . Results:The linear range of osthole was 2. 0-80. 0 μg· ml-1(r=0. 999 1). The average recovery of osthole was 97. 5%(RSD=1. 95%,n=6). Conclusion:The method is simple, rapid and accurate, which can be used for the determination of osthole in Baicao Fuyanqing suppositories.

Objective:To establish a rapid and sensitive method for the determination of carbendazim in honeysuckle flowers by LC-MS/MS. Methods:The sample was extracted by acetic ether. The mass spectrometer was operated in the positive ionization elec-trospray ( ESI) mode using selection ion monitoring ( SIM) . The transition m/z 192→160 was used to quantify carbendazim. Results:The method had a satisfactory linearity within the range of 50-5 000 ng·ml-1 for carbendazim (r=0. 999 4), the limit of detection (LOD) was 2. 0 ng·ml-1, and the mean recovery was 93. 2%. Conclusion:The method of LC-MS/MS is sensitive, simple and ac-curate,which is proved to be suitable for the determination of carbendazim in honeysuckle flowers.