Objective To study the significance of microvascular invasion to the neogrow in clinically nonmetastatic renal cell carcinoma (RCC). Methods Between 1989 and 1996,70 patients (mean age 54) were followed up for 1 to 7 years after radical nephrectomy for clinically localized RCC. Among them,there were 5 PT 1,50 PT 2,14 PT 3 and 1 PT 4 and histologically 7 G 1,38 G 2,19 G 3 and 6 G 4.The mean tumor diameter was 7.2 cm.The slides were stained with hematoxylin and eosin,elastin stains and periodic acid Schiff. then,the presence or absence of clinically inapparent vascular invasion,the relevance of microscopic vascular invasion to conventional tumor stage,grade and tumor diameter,et al were studied. Results Of the 70 patients analyzed,24(34.3%) had microvascular invasion,while 46 had none on microscopic examination.Of the microscopic vascular invasion group (11/24,45.8%),7 subsequently died of cancer recurrence,2 noncancer related death,4 alive with metastatic disease while only 4/46(8.7%) without microscopic vascular invasion presented with disease progression.Chi-square test showed statisticaly significant difference between stage,grade,tumor diameter and presence or absence of microscopic vascular invasion. A multivarite analysis was performed considering the impacts of age,PT stage,tumor grade,tumor diameter and microscopic vascular invasion on disease progression,an increase in statistical significance was confirmed with Coxs proportional hazards model(P=0.0062,RR=0.378). So, microscopic vascular invasion seems to be the most important predictor of progression in RCC. Conclusions In patients underwent radical nephrectomy for clinically nonmetastatic RCC,microvascular invasion may be another important prognostic marker and this makes us considering the presence of microscopic vascular invasion in RCC might be another pathological subcategory to predict the prognosis of RCC and can be used to choose whether early adjuvant therapy is necessary.

Purpose:To evaluate angiogenesis and its correlation to recurrence of transitional cell carcinoma of the bladder.Methods:Specimens from 25 patients with transitional cell carcinoma of the bladder were assayed using immunohistochemical technique to examine the expression of factor Ⅷ related antigen, basic fibroblast growth factor(bFGF) and vessel endothelial growth factor(VEGF), as well as the microvessel density.Results:The positive rate of VEGF and bFGF was 48% and 28%. The intratumor microvessel density(iMV) of tissues with positive staining was significantly higher than those with negative staining(P＜0.05),and the iMV of patients with recurrence was significantly higher than those without recurrence(P＜0.05).Conclusions:iMV correlated positively with recurrence and the expression of VEGF and bFGF was related to the function of angiogenesis in transitional cell carcinoma of the bladder.

Objective To observe the anti-inflammatory and analgesic effects of CNZ,an optimized prescrition of Liushen Wan based on our previous studies,so as to provide some pharmacological evidence for its further clinical use. Methods Both acetic-acid-induced increased mouse vascular permeability and carrageenan-induced mouse footpad edema were used to study the anti-inflammatory activity of CNZ.Meanwhile,its analgesic activity was evaluated in mice model of pain induced by thermal stimulus and acetic acid.The above activities were compared with those of Liushen Wan.Results CNZ significantly reduced acetic-acid-induced dye leakage at the doses of 16,32 and 64mg/kg after single oral administration.Meanwhile,CNZ at the two higher doses also markedly inhibited carrageenan-induced foot- pad edema,which exerted the strongest effect at 3 hours after carrageenan injection,and lasted over 5 hours.On the other hand,CNZ remarkably suppressed acetic-acid-induced writhing response at low,moderate-and high-doses, and significantly prolonged pain threshold in hot plate assay at moderate-and high-doses 0.5 hour after oral adminis- tration,lasting over 3 hours.Overall,its potency was similar to that of Liushen Wan.Conclusion CNZ has significant anti-inflammatory and analgesic activities.

OBJECTIVETo explore the correlation between the content of lecithin mass and white blood cells (WBC) of the expressed prostatic secretion (EPS) and the concentration of serum PSA in patients with prostatitis, and to study the difference in serum PSA concentration between patients with bacterial prostatitis and those with nonbacterial prostatitis.

METHODSThe serum PSA concentration in 62 patients with prostatitis and 22 controls were measured with ELISA method. The correlation between the content of lecithin mass and WBC of the EPS and the elevation of serum PSA was analyzed. And the serum PSA concentration of bacterial prostatitis (9 patients) and that of nonbacterial inflammatory prostatitis (53 patients) were compared.

RESULTSThe mean concentrations of serum PSA in the prostatitis and the control groups were (1.79 +/- 0.68) microg/L and (0.63 +/- 0.29) microg/L, respectively. The difference of the serum PSA concentration was significant between the prostatitis and the control groups (P < 0.001) as well as between the groups with higher and lower WBC contents in EPS (P < 0.05), but not between the groups with higher (27 patients) and lower (35 patients) lecithin mass contents in EPS (P > 0.05), nor between the groups of bacterial prostatitis and nonbacterial prostatitis (P > 0.05).

CONCLUSIONProstatitis may cause the elevation of serum PSA concentration. The elevated serum PSA correlates with the content of white blood cells in EPS, but not with the content of lecithin mass in EPS, nor with the type of prostatitis, either bacterial or nonbacterial.

Adult ; Humans ; Leukocyte Count ; Male ; Middle Aged ; Phosphatidylcholines ; analysis ; Prostate ; secretion ; Prostate-Specific Antigen ; blood ; Prostatitis ; metabolism