Objective To measure serum surfactant protein (SP) A and D levels in patients with interstitial lung disease associated with rheumatoid arthritis (RA).Methods Serum SP-A and SP-D levels of RA,RA-ILD patients and healthy controls were assessed using a sensitive enzyme-linked immunosorbent assay (ELISA).The relationship between SP-A and SP-D and RA-ILD was analyzed.The serum SP-A and SP-Dpositive rate was calculated for the three groups.The correlation between SP-A and SP-D with RF,anti-CCP,antinuclear antibody,antikeratin antibody,anti-perinuclear factor,C-reactive protein,erythrocyte sedimentation rate,were analyzed.Mean value of groups were compared with variance analysis,Spearmam rank correlation test was used for correlation analysis.Results The levels of serum SP-A in RA-ILD patients and RA patients as well as in healthy controls were [ (51.2±9.2),(25.9±2.6),( 15A±0.3 ) μg/L] respectively.The level of serum SP-D of the three groups was [ ( 42.5 ±8.1 ),(20.8 ± 1.5 ),( 16.6±0.8 ) μg/L ] respectively.The levels of serum SP-A and SP-D in patients with RA complicated with ILD were higher than those simple RA patients and healthy controls (P＜0.05).The levels of serum SP-A and SP-D in patients with RA were not significantly higher than those in healthy controls (P＞0.05).The positive rate of serum SP-A and SP-D in RA-ILD patients were significantly higher than those in simple RA patients and healthy controls.The positive rate of serum SP-D of RA-ILD patients was higher than that of SP-A.The levels of serum SP-A and SP-D in patients with RA complicated with ILD were correlated positively with age,C-reactive protein.The level of serum SP-D was correlated positively with RF,anti-CCP,antikeratin antibody.There was no correlation between the level of serum SP-A and SP-D with RA-ILD and antinuclear antibody,antiperinuclear factor,erythrocyte sedimentation rate.Conclusion The levels of serum SP-A and SP-D are correlated with RA-ILD and may be useful markers for ILD in patients with RA.These two paramenters may be helpful to early diagnosis of RA-ILD.The Serum SP-D levels are more sensitive in predicting the development of RA-ILD than other parameters and can help in assessing the severity of lung damage.

OBJECTIVETo establish an effective protocol for plant generation and induce polyploidy of Morinda offcinalis.

METHODCallus was induced from immature embryo of M. officinalis and polyploidy was inducted by using colchicine treatment method. Chromosome was detected by flow cytometry.

RESULT AND CONCLUSIONThe highest induction rate of polyploidy was 18.40%, which was obtained with 500 mg x L(-1) colchicine treatment for 5 days. Roots of polyploid were bigger than diploid. Advantages of using immature embryo as explants are easy for sterilization, higher rate of callus induction and low degree dedifferentiation. The induced polyploidy of M. officinalis may have a value for spread of cultivation.

Chromosomes, Plant ; genetics ; Morinda ; genetics ; growth & development ; Polyploidy ; Tissue Culture Techniques ; methods

Objective To explore the incidence of chronic postsurgical pain (CPSP) of video assisted thoracic surgery(VATS) and its influencing factors.Methods Totally 216 patients who received elective VATS in a class Ⅲ grade A hospital between January and June 2016 were selected by convenience sampling. Their pain level was assessed with Visual Analogue Scale (VAS) in 1 to 3 days after surgery. The patients were then investigated with Simplified McGill Pain Questionnaire (SF-MPQ) in 1 to 3 months after surgery,with the features of CPSP analyzed and 11 related risk factors statistically analyzed.Results The incidence of CPSP in 3 months after surgery in the 199 patients who received follow-up visits was 49.20%,of which 18.37% suffered moderate pain,and 32.65% felt constant pain. Age (OR=2.16) and pain level in 1-3 days after surgery (OR=2.25) were independent risk factors to CPSP (P<0.05).Conclusions CPSP occurred in a certain proportion of patients who received VATS. Actively and effectively controlling acute post surgical pain in especially young patients may help to reduce the incidenc of CPSP.

Objective To explore the perioperative psychological behavior interventions for patients with postoperative anxiety and pain of gastrointestinal tumor. Methods We selected 110 patients with gastrointestinal cancer during surgical treatment from gastrointestinal surgery department of Anhui provincial hospital from April 2014 to October 2014 and divided them into experimental group (n=58) and control group (n=52). The patients of control group were used conventional care while the patients of experimental group received the psychological behavior therapy, relaxation therapy and music therapy within 72 hours after surgery for evaluation of postoperative pain and anxiety on the basis of conventional nursing, and we carried on the comparison of two groups of patients with postoperative hospital stay. Results Two groups of patients with SAI and TAI were no statistical difference at time of admitting to hospital (P>0. 05) ; the SAI and TAI of the experimental group patients in postoperative one day and three days after operation are lower than those of the control group, and the difference between the two groups after surgery was statistically significant (P<0. 05);the time effect on the result without statistical significance (P>0. 05);NRS score of different time points after surgery within two groups showed that the experimental group NRS scores were lower than those of the control group, and the differences were statistically significant between two groups (P<0. 05); the time effect on the result without statistical significance (P >0. 05); the experimental group patients in hospitalization days and postoperative hospitalization days were shorter than these of the control group, the difference between the two groups have statistical significance (P <0. 05). Conclusions Relaxation therapy and music therapy can significantly improve the patient′s perioperative anxiety, reduce the pain after surgery. For gastrointestinal cancer patients perioperative health of body and mind and postoperative rehabilitation, it has a positive role.