OBJECTIVETo compare the contents and composition of volatile oil of Wenyujin (steamed root of Curcuma wenyujin) Wenezhu (steamed rhieome of Curcuma wenyujin) and Pianjianghuang (rhizome of Curcuma wenyujin).

METHODThe volatile oils of three herbal medicines obtained by steam distillation were determined according to the method described in the appendix XD in Chinese pharmacopoeia and identified by GC-MS.

RESULTThe average contents of volatile oil of Wenyujin, Wenezhu and Pianjianghuang were 0.56, 1.99 and 0.94 microL x g(-1), respectively. A total of 60 compounds were identified by GC-MS. The amounts of chemical components separated and identified in Wenyujin, Wenezhu and Pianjianghuang were 41, 41 and 40, respectively. Three medicines share a great deal of similarity, chemically and genetically. Low boiling point essential oil compositions Wenyujin and Wenezhu were similar. While the higher boiling point essential oil compositions of Wenyujin and Wenezhu were similar. And the essential oil compositions of Wenyujin and Pianjianghuang were distinct.

CONCLUSIONAlthough coming from the same origin, the composition of essential oil from different part of the plant or various process were discrepant, which lead to rather distinct clinical efficacy.

Curcuma ; chemistry ; Gas Chromatography-Mass Spectrometry ; methods ; Herbal Medicine ; Oils, Volatile ; chemistry ; Plant Extracts ; chemistry ; Plant Roots ; chemistry ; Quality Control ; Rhizome ; chemistry

OBJECTIVETo establish and revise the quality specification of Curcumae Radix.

METHODTLC identification was carried out by the method in Appendix VI B in Chinese pharmacopoeia (2005th Edition) and the contents of volatile oil was determined by the method describing in Appendix XD.

RESULTTwenty-four Samples from four different curcuma species were performed TLC qualitative analysis and volatile determination, respectively.

CONCLUSIONThe established method can be used for the quality control of Curcumae Radix.

Chromatography, Thin Layer ; Curcuma ; chemistry ; Drugs, Chinese Herbal ; analysis ; Oils, Volatile ; analysis

Objective To explore the effect and complications of ventricle peritoneal shunt n pediatric hydrocephalus. Methods The clinical data was analysed in 30 cases of pediatric hydrocephalus. Results A total of 27 cases (90%) of pediatric hydrocephalus were found reduction of ventricle. 3 cases were found no obvious reduction of ventricle. Post-operative complications in 3 cases (10%), subcutaneous liquid collection in 2 cases (6.67%), diverter pump in 1 case (3.33%). No patients were found infection. Conclusion Strict sterilizing procedures andhigh quality and attention-to-detail is the key to reduce postoperative complications and improve surgical outcomes.

Objective:To investigate the surgical method and curative effect of resection of craniopharyngioma through sub-frontal longitudinal fissure duramater-keyhole approach.Methods:The clinical data, imaging data of 179 patients with craniopharyngioma treated by sub-frontal longitudinal fissure duramater-keyhole approach from January 2017 to January 2021 in Sanbo Brain Hospital Capital Medical University were studied retrospectively.Results:Through this approach, total resection in 175 cases (97.77%), near total resection in 2 cases (1.12%), partial resection in 2 cases (1.12%); drilling of the tuberculum sellae in 31 cases (17.32%); the pituitary stalk was reserved in166 cases (92.74%), preserved in 13cases (7.26%); papillary craniopharyngioma in 27 cases (15.08%), ameloblastic craniopharyngioma in 152cases (84.92%); postoperative visual acuity improved in 65 cases (36.31%), not improved in 53 cases (29.61%), decreased in 6 cases (3.35%); electrolyte disorder in 101 cases (56.42%), polydipsia and polyuria in 65cases (36.31%), cerebrospinal fluid rhinorrhea in 3 cases (1.68%), hydrocephalus in 6 cases (3.35%). Postoperative hematoma in 2 (1.12%) cases and cerebral infarction in 6 cases (3.35%), intracranial infection in 8 cases (4.47%), postoperative disturbance of consciousness in 4 cases (2.23%) and death in 3cases (1.68%).Conclusions:Sub-frontallongitudinal fissure duramater-keyhole approach is an effective combination of minimally invasive and delicate surgery principle. It is a safe and effective method for the treatment of craniopharyngioma.

Objective:To analyze the surgical (anatomical) approach of craniopharyngioma based on the concept of anterior central space of brain base.Methods:The clinical data of MRI images of 10 cases of craniopharyngioma who treated in Sanbo Brain Hospital, Capital Medical University from January 2021 to December 2021 were analyzed retrospectively. The brain cisterns involved in the growth of craniopharyngioma were analyzed from the perspective of anterior central space of brain base. Meanwhile, the surgical approach and the basis of selection were discussed.Results:Among all the surgical approaches that can reach the anterior central space of the brain base, the fronto-basal interhemispheric approach was the best.Conclusions:The concept of anterior central space of the brain base is a new observation and interpretation of the anatomy of the brain base from a new perspective, and then create a new concept of brain base surgery. As a new method to analyze the surgical anatomy of this area, its inclusion has important clinical significance.

Objective:To study the cause of frontal sinusitis and the prevention methods after opened frontal sinus in the trans-frontal approach surgery.Methods:Retrospectively analyze 18 cases of frontal sinusitis secondary to the trans-frontal approach surgery in Sanbo Brain Hospital, Capital Medical University from January 2016 to December 2020. The main symptoms include headache, stuffy nose, skin redness and skin abscess in frontal. The mean history was 3.2 years, all the patients were performed surgical treatment, including 5 patients undergone trans-nasal trans-sphenoid sinus operation, 14 patients undergone debridement surgery following the original surgical approach. The cause of frontal sinusitis were analyzed statistically.Results:All the patients were totally cured post operation, the standard included no frontal sinus inflammation symptoms, no imaging characters of frontal sinusitis.Conclusions:Proper management of opened frontal sinus in trans-frontal approach is very important for preventing the frontal sinusitis. Completely remove the frontal sinus mucosa, avoid using bone wax, chemical glue stuffing, which can effectively prevent the frontal sinusitis.

Objective:To explore the surgical method and clinical effect of craniopharyngioma resection through the cockscomb approach.Methods:A retrospective case-control study was conducted from December 2010 to May 2018.The clinical data, imaging data and follow-up data of 101 patients with craniopharyngioma confirmed by pathology by pathology after resection of the frontal floor longitudinal fissure through the crow′s comb approach were performed in Department of Neurosurgery, Sanbo Brain Hospital Capital Medical University.The total tumor resection rate, mortality and complications were analyzed by statistical methods.Results:Through this approach, the total resection rate of tumor was 90.1%(91/101), and the mortality was 1.9%(2/101). The most common postoperative complications were disturbance of sodium metabolism (54.5%(55/101)).Conclusion:Sub-frontal longitudinal fissure crista galli approach can safely and thoroughly remove the tumor and avoid the injury of the hypothalamus and its surrounding tissues as far as possible.

Purpose: NUF2 has been implicated in multiple cancers recently, suggesting NUF2 may play a role in the common tumorigenesis process. In this study, we aim to perform comprehensive meta-analysis of NUF2 expression in the cancer types included in the Cancer Genome Atlas (TCGA). Materials and Methods: RNA-sequencing data in 31 cancer types in the TCGA data and 11 independent datasets were used to examine NUF2 expression. Silencing NUF2 using targeting shRNAs in hepatocellular carcinoma (HCC) cell lines was used to evaluate NUF2’s role in HCC in vitro and in vivo. Results: NUF2 up-regulation is significantly observed in 23 out of the 31 cancer types in the TCGA datasets and validated in 13 major cancer types using 11 independent datasets. NUF2 overexpression was clinically important as high NUF2 was significantly associated with tumor stages in eight different cancers. High NUF2 was also associated with significantly poorer patient overall survival and disease-free survival in eight and six cancers, respectively. We proceeded to validate NUF2 overexpression and its negative association with overall survival at the protein level in an independent cohort of 40 HCC patients. Compared to the non-targeting controls, NUF2 knockdown cells showed significantly reduced ability to grow, migrate into a scratch wound and invade the 8 μm porous membrane in vitro. Moreover, NUF2 knockdown cells also formed significantly smaller tumors than control cells in mouse xenograft assays in vivo. Conclusion NUF2 up-regulation is a common feature of many cancers. The prognostic potential and functional impact of NUF2 up-regulation warrant further studies.

Carcinogenesis ; Cell Transformation, Neoplastic ; Hepatoblastoma ; Humans ; Liver ; Liver Neoplasms ; Organoids ; YAP-Signaling Proteins