Objective To observe the effects of different doses of Sijunzi decoction(SJZD) on soleus(SOL) atrophy in tail-suspended rats.Methods Weightlessness was simulated by tail suspension in female Sprague-Dawley rats.Six rats in each group were assigned to a 5-group design randomly: control(CON);hind limb unloading(HLU) plus intragastric water instillation(HLU-W),as well as plus intragastric high dose(HLU-H),middle dose(HLU-M) and low dose(HLU-L) SJZD instillation respectively.Immunohistochemical method was used to observe the effects of them on expression of myosin heavy chain(MHC) isoforms in soleus muscle.Results Compared with HLU-W,muscle wet weight(MWW) of SOL in high and middle dose group increased by 33.11%and 37.57%respectively,as well as no significant difference with CON;three doses of SJZD groups increased the percentage of type Ⅰ myosin by 34.04%~54.90% and its cross sectional area(CSA) by 8.50%~36.86%,moreover SJZD of middle dose group showed no significant difference with that of CON group.Conclusion Three doses of SJZD groups can significantly prevent soleus atrophy induced by simulated weightlessness,and middle dose is the more effective one than high and low dose.

BACKGROUND: In our previous studies, zinc-calcium-phosphate (Zn-Ca-P) coating has been successful y prepared on the surface of AZ31 magnesium al oy, which improves the corrosion resistance of the al oy. OBJECTIVE: To analyze the hemocompatibility of Zn-Ca-P coated AZ31 magnesium al oy in vitro. METHODS: Zn-Ca-P coating was prepared on the surface of AZ31 magnesium al oy using chemical transformation. Afterwards, the characteristics of the Zn-Ca-P coating were investigated using scanning electron microscope, and X-ray diffraction analyzed its components. Besides, hemocompatibility in vitro was evaluated by platelet adhesion assay, dynamic clotting time test and hemolysis test. RESULTS AND CONCLUSION: Under scanning electron microscope, a flower-like coating was formed ont surface of the AZ31 magnesium al oy, with the main chemical component of insoluble phosphates Zn3(PO4)2?4H2O. And some round distortionless blood platelets adhered to the surface of AZ31 magnesium al oy, with no pseudopodia. In dynamic clotting time test, the long curve of Zn-Ca-P coating tended to descend indicating better anticoagulant activity. And the hemolysis rate was below 5%. In conclusion, Zn-Ca-P coating has good hemocompatibility in vitro.