Objective To observe the clinical curative effect of the joint application of Vitapex paste and AH plus,which cured the combined periodontic -endodontic lesion .Methods 72 patients of 113 teeth with the com-bined periodontic-endodontic lesion were chosen ,which were randomly divided into the two groups ,with all complete periodontal initial therapy and routine root canal preparation and dressing at the same time .The observation group included 36 cases of 58 teeth,when the first time to see a doctor ,injected Vitapex paste to the periapical tissue and periodontal tissue sparse area for 1 -2weeks.The sceond time,the tap root canal was closely filled withc AH plus paste and gutta-percha.The control group concludes 36 cases of 55 teeth,with the traditional method,the tap root canal was filled with AH plus paste and gutta -percha directly .Then observation of postoperative emergency response within 1 week,of periodontal clinical indicators (including the PD,BOP,PLI) in 1 month,3 months,6 months after periodontal initial therapy ,and of the long-term curative effect after 1 year.Results 1 week after root canal filling , there were 48 cases no reaction , 9 cases mild reaction and 1 case severe reaction in the observation group , while 47cases no reaction ,7 cases mild reaction and 1 case severe reaction in the control group .There was no statistical difference of evaluation of emergency response between the two groups (χ2 =0.002,P>0.05).Compared with the situations before treatment ,PD,BOP and PLI were all reduced significantly in 1,3 and 6 months after periodontal ther-apy (P<0.05).There was no significant differences of PD ,BOP and PLI between the two groups before treatment (t=0.050,0.019,0.004,all P>0.05),while 1 month (t=2.581,5.984,1.870,all P<0.05),3 months (t=4.806,7.640,1.034,all P<0.05) and 6 months (t=4.612,8.043,1.167,all P<0.05) later,there was statistical difference of decreased value between the two groups .The 1 year follow up showed that significant efficiency in the observation group was 46.55%,and total efficiency was 91.38%,while significant efficiency 27.27% and total effi-ciency 70.91%in the control group ,and there were statistical differences of significant efficiency and total efficiency between the two groups (χ2 =16.761,21.061,all P<0.05).Conclusion With the joint application of Vitapex paste and AH plus ,and two method curing the combined periodontic -endodontic lesion ,there was no evident differ-ence compared with the traditional short -term emergency response .By long-term observation ,found that this method could effectively promote the healing of periapical tissue and periodontal tissue ,and that curative effect is remarkable compared with the traditional method .

OBJECTIVE To understand mecA gene distribution in Staphylococcus aureus and its role in antibiotics-resistance.METHODS In this study,a total of 47 S.aureus strains were isolated from hospitalized patients.Agar disk diffusion test was conducted to determine the resistance of S.aureus to antibiotics.The DNA of these strains were extracted and purified.The mecA gene was tested by PCR and the relation between the mecA gene and antibiotics-resistance was analyzed.RESULTS Of 47 strains,33(70.2%) were MRSA.Of 33 MRSA,only 3 strains were susceptible to glycopeptides antibiotics.Only 2 strains(14.3%) of 14 MSSA were susceptible to all of the 12 antibotics.The results of PCR revealed that 32 out of 33 MRSA(97.0%) carried mecA in their genome.One strain was mecA gene negative.Among 14 MSSA,3(21.4%)strains carried mecA gene.CONCLUSIONS The isolation rate of MRSA in S.aureus is high.The resistance to antibiotics of MRSA is popular Glycopeptides antibiotics.Most of MRSA carry mecA gene,which plays an important role in antibiotics-resistance.Fewer MSSA carry mecA gene.