Objective To investigate the selection of flaps for the reconstruction of facial skin defects.Methods A total of 40 patients with facial skin defects were treated from February 2011 to February 2015 in our hospital,and they were given different surgery methods ac-cording to the different facial skin defect sizes.Minor defects were primarily treated by direct suturing;medium-sized defects were treated by local skin flap transposition or island skin flap;and larger defects were treated by expanded skin flap.Results All patients were followed up for 1 to 2 years.All the flaps survived with good color and texture match.All the scars healed well which were almost invisible.There was no obvious deformity in the donor and recipient sites.In addition,there was no lesion recurred.Conclusion Select suitable flaps for the recon-struction of facial skin defects according to the face defect area could get reliable blood supply of the flap,inapparent incision scar and a high level of satisfaction.

Objective To sum up our clinical experience in interventional treatment of 143 cases of Budd-Chiari syndrome. Methods This study included 92 males and 51 females, aged from 6 to 65 years old with an average of 34.8 years. The pathologic types were composed of complete occlusion of inferior vena cava (IVC) (71), IVC stenosis (36), IVC membrane occlusion with a hole (29), membrane occlusion of hepatic vein (HV) (3), IVC thrombosis (4), and IVC lesions forementioned combined with HV occlusion (14). Therapeutic methods included that I: Percutaneous transinferior vena cava membranotomy and occlusion dilatation (PTA) (77); II: IVC PTA with stent (62); III: Percutaneous transhepatic vein recanalization (3); IV: IVC thrombolysis through a catheter (4); V: Additional operation after intervention (16). Results The range of reduced IVC pressure was (3 ～ 29) cmH 2O with the mean pressure being 12.1 cmH 2O. Complications occurred in 8 cases, including pulmonary embolism (PE), stent migration and HV occlusion after IVC stent (2 cases respectively), cardiac tamponade and hemothorax (1 case repectively). 2 cases died of PE and 3 cases died of hepatic coma after meso-caval shunt,the death rate being 3 5% . A follow-up study showed the recurrence rates were 10.4% in IVC PTA cases and 1.6% in IVC PTA with stent cases respectively, and no recurrence was found in other cases. Conclusions ① PTA is the first choice for localized lesions without fresh thrombus. ② For those with elastic recoil or recurrence, stent is suggested. ③ For those with both IVC lesions and HV occlusion, the additional operation to reduce portal hypertension is needed after IVC intervention.

AIM:To investigate the relationship between the number of dopaminergic neurons and the locomo-tor behavior of animals,and to provide a reference basis for the modeling of mice with different stages of Parkinson disease(PD)and different types of locomotor deficits based on 6-hydroxydopamine(6-OHDA)injection.METHODS:We in-duced lesions in the substantia nigra pars compacta(SNc)by administering various doses of 6-OHDA(3 g/L,6 g/L,and 12 g/L)to create PD mouse models with differing degrees of injury,thereby mimicking the various stages of PD progression observed in patients(early,moderate and advanced stages).On the 14th day post-surgery,we evaluated the behavioral deficits of the mouse models using the rotarod test,pole test,beam traversal test,open field test,and gait analysis.Fur-thermore,the quantification of tyrosine hydroxylase(TH)-positive neurons within the SNc and TH-stained dopaminergic terminals in the corpus striatum caudate-putamen(CPu)was conducted utilizing immunofluorescence staining techniques to assess brain tissue damage.RESULTS:Compared to the control group,the number of dopaminergic neurons in the SNc was significantly reduced in both the high-dose group(P<0.05)and the medium-dose group(P<0.05)following 6-OHDA injection,demonstrating a dose-dependent effect(Spearman correlation,P<0.01).Similarly,the dopaminergic terminals in the CPu were significantly diminished in the high-dose group(P<0.01)and the medium-dose group(P<0.05).Behavioral tests revealed that mice in the high-dose group exhibited severe impairments in motor coordination and hindlimb balance,as evidenced by reduced rotarod test times,gait abnormalities,and asymmetrical forelimb use in the cylinder test.In contrast,mice in the medium-and low-dose groups displayed only mild declines in limb coordination,while their autonomous motor abilities and gait indices remained largely unaffected.CONCLUSION:The results reveal a dose-dependent effect on dopamine neuron damage,with higher doses causing the severest damage.Unexpectedly,signifi-cant locomotion impairments were only manifested in the high-dose group.This suggests that a mouse model induced by higher 6-OHDA dose is effective for studying PD and associated dyskinesia.Conversely,animal models with low to medi-um doses can be useful for exploring the early stages of PD locomotion symptoms.