1.Analysis on Prescription Quality Using Access Software
China Pharmacy 2007;0(34):-
OBJECTIVE:To enhance the efficiency and the standardization degree of the statistical analysis of prescription quality. METHODS: Taking the Prescription Management Method as criterion, Access software management system was designed and developed for the statistical analysis of the outpatient prescriptions. RESULTS & CONCLUSIONS: The Access software is powerful in function, highly standard, easy to operate and transferable etc, and it greatly enhanced the efficiency and the quality of the statistical analysis of prescription quality and contributed to rational drug use.
2.Simultaneous Determination of the Contents of Salicylic Acid and Nitrofural in Fu-La Pastes by UV Spectrophotometry
Yanpeng WANG ; Qingyuan HOU ; Ting ZHANG
China Pharmacy 2001;0(07):-
OBJECTIVE: To establish a method for the simultaneous determination of the contents of salicylic acid and nitrofural in Fu-La pastes.METHODS: A dual-wavelength spectrophotometry was adopted for the determination of the contents of salicylic acid and nitrofural with water used as solvent.The content of salicylic acid was determined with the detective wavelength set at 296.6 nm and the reference wavelength set at 314.4 nm.The content of the nitrofural was determined directly with the detective wavelength set at 375.0 nm.RESULTS: The linear ranges of salicylic acid and nitrofural were 10.77~43.08 ?g?mL-1(r=0.999 0) and 2.58~10.32 ?g?mL-1(r=1.000 0),respectively;and their average recovery rates were 99.55%(RSD=0.47%) and 100.03%(RSD=0.80%),respectively.CONCLUSION: The method is simple,rapid and accurate,and suitable for the quality control of Fu-La pastes.
3.Experience of Flow Management on the Use of Anesthetic Drugs
Qingyuan HOU ; Jianrong LIU ; Jichuan WANG
China Pharmacy 2007;0(25):-
OBJECTIVE: To ensure legal,rational and safe use of anesthetic drugs.METHODS: Based on the related laws and regulations as well as the actuality,the regulatory regime on the use of anesthetic drugs was set and enforced.RESULTS: The regulations about the use of anesthetic drugs were able to be implemented satisfactorily,and the regulatory regime has changed the abstract management connotation into rapid process.CONCLUSION: It's operative and efficient to adopt flow management on the use of anesthetic drugs.
4.Applications of Phospho-histone H2AX Enzyme-Linked Immunosorbent Assay in Vitro Genotoxicity Evaluation of Cigarette Smoke
Liwei FU ; Huan CHEN ; Jin YANG ; Hongwei HOU ; Qingyuan HU
Chinese Journal of Analytical Chemistry 2014;(3):320-325
Phospho-histone H2AX(γH2AX) has been widely used in vitro genotoxicity evaluation of ionizing radiation, carcinogenic substances and cigarette smoke as an important biomarker of DNA double stranded breaks ( DSBs) . The study developed an enzyme-linked immunesorbent assay for detection of the content ofγH2AX in cells to evaluate the genotoxicity of cigarette smoke. The study exposed CHO cells with cigarette smoke total particulate matter ( TPM) and cigarette smoke condensate ( CSC) of different dose, dose-effect relationship between smoke exposure and DNA damage and the poisonous difference of cigarette smoke components has been investigated by detecting the content change ofγH2 AX in cells under different exposure time and dose. In addition, reactive oxygen species ( ROS) in cells were also detected to study the mechanism of cigarette smoke exposure induced DSBs. The experiment result showed that the required time for the level ofγH2AX in cells grew to the peak prolonged with increased exposure dose. Once the level of γH2AX in cells grew to the peak value, then it decreased slowly; the level of γH2AX in cells rised with increasing dose of cigarette smoke;The effect of TPM on the content change of γH2AX was more than CSC; Moreover, smoke exposure could induce concentration increase of ROS in cells, and a good correlation of content change of ROS and γH2AX in cells were found, free radicals in cigarette smoke may be one major cause of DSBs.
5.Progress on Molecular Mechanism of Phosphorylation/Dephosphorylation and Detection Technology of γH2 AX
Sen ZHANG ; Lujuan LIU ; Huan CHEN ; Hongwei HOU ; Qingyuan HU
Chinese Journal of Analytical Chemistry 2016;(8):1291-1299
Since γH2 AX was firstly found in 1998 , it has been one of the most important scientific topics and research tools in the related scientific fields. At present, a series of advanced testing methods and analytical technologies have been developed, which exhibited a quite attractive application prospect in the area of life science and medical science. This paper reviewed the latest progress about γH2AX in terms of molecular mechanism of phosphorylation/dephosphorylation, development of testing technologies, and the related applications.
6.Research Progress in Analysis of Small Molecule Metabolites inBio-matrices by Stable Isotope Coded Derivatization Combiningwith Liquid Chromatography-Tandem Mass Spectrometry
Yunlu HE ; Yanbo LUO ; Huan CHEN ; Hongwei HOU ; Qingyuan HU
Chinese Journal of Analytical Chemistry 2017;45(7):1066-1077
Stable isotope coded derivatization (ICD) is an isotope labeling technique for specific functional groups of the target analytes through chemical derivatization.ICD combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) enables systematically analyzing the biomolecules with the same labeling reaction characteristic.ICD technique can effectively solve the limited sensitivity in complex bio-matrices analysis and unavailability of isotope internal standards in quantitative analysis.In recent years, ICD technique has been widely used in metabolomics research field.Based on this background, the ICD technique and the design of ICD reagents are briefly described in this review.The recent advances in ICD reagents for carboxyl, amino, carbonyl, thiol and hydroxyl groups and their applications in the analysis of small molecule metabolites in bio-matrices with LC-MS/MS are reviewed.
7.Tightening Control on Prescriptions of Antibiotics and Narcotics Drugs with Hospital Information System
Qingyuan HOU ; Yanhong ZHAO ; Kai GU ; Yanpeng WANG
China Pharmacy 2005;0(22):-
OBJECTIVE:To facilitate the hierachical management and use of antibiotics and tighten control on prescription right on the narcotics drugs.METHODS:Hospital informational system has contributed to the informatization management on supervision of the prescriptions of antibiotics and narcotics.RESULTS:After half a year's practice of informatization management,remarkable outcome has achieved in that the practice of hierachical management and use of antibiotics has been improved significantly,and the management on the prescription right of narcotics drugs became more simple and reliable.CONCLUSIONS:Adopting hospital information system to tighten control on the prescriptions of antibiotics and narcotics drugs is a good management model in which various abnormal prescription behaviors can be effectively warned of and guard against from the very beginning.
8.Simultaneous Determination of N3-methyladenine and N3-ethyladenine in DNA by Liquid Chromatography-Tandem Mass Spectrometry
Yongfeng TIAN ; Hongwei HOU ; Xiaotao ZHANG ; Yong LIU ; An WANG ; Qingyuan HU
Chinese Journal of Analytical Chemistry 2014;(8):1201-1205
A liquid chromatography-tandem mass spectrometry ( LC-MSMS ) method has been developed for the simultaneous determination of N3-methyladenine ( N3-MeA ) and N3-ethyladenine ( N3-EtA ) in calf thymus DNA. The DNA samples has been purified and enriched by cation exchange cartridge ( Waters Oasis MCX) . d3-N3-MeA and d5-N3-EtA were used as isotope internal standard. The DNA samples were injected with autosampler. The injected volume was 3 μL and analysis time was 13 min. The sample separation was carried out on hydrophilic interaction chromatograph ( Waters XBridge HILIC ) with 10 mmol/L ammonium formate-acetonitrile (5:92, V/V, pH=4. 0) as mobile phase. The flow rate was set at 250 μL/min. Mass spectrometry was performed by electrospray ionization ( ESI ) with multi-reactions monitoring ( MRM ) . The optimized operation conditions of MS were as follows: nebulizer gas 369 Pa; curtain gas 185 Pa, turbo ionspray temperature 400 ℃, ionspray voltage 5500 V, dwell time 40 ms. The limits of detection were 0. 043 and 0. 007 μg/L for N3-MeA and N3-EtA, respectively. The recoveries were between 87. 8% and 103. 0%for N3-MeA and N3-EtA. This method was successfully applied to the determination of N3-MeA and N3-EtA in calf thymus DNA by cigarette smoke condensate ( CSC) exposure. This method is appropriate for routine analysis and accurate quantification of N3-MeA and N3-EtA by CSC exposure.
9.MRI features of Hirayama disease at different cervical flexion angles
Hui GAO ; Hongbin HAN ; Xiaojuan XU ; Chao HOU ; Qingyuan HE ; Dongsheng FAN ; Yu FU ; Yu SUN
Chinese Journal of Radiology 2010;44(6):653-656
Objective To analyze MRI features of different cervical flexion positions in Hirayama disease (HD) and discuss the effects on these features by different cervical flexion angles.Methods The cervical MR images of neutral and different flexion positions (20°, 25°, 30°, 35° ,40°) of 20 patients, who were clinically diagnosed as HD,were studied.At flexion positions, the appearance of anterior shifting of the posterior wall of the cervical dural canal and widening of epidural space was recorded.The maximum sagittal diameters (d) of widened cervical epidural space and the cervical canal sagittal diameters (D) on the same level were measured to calculate d/D value for quantitative evaluation of the two signs.Comparisons of appearance of the signs among different flexion positions were made using F/sher's exact test.Repeated measures analysis of variance (rmANOVA) was used to compare mean d/D values among groups with different positions, and paired comparisons were also performed.Results The appearance of anterior shifting of the posterior wall of the cervical dural canal were different between 20° group (70%, 14/20) and other 4 larger angles groups (100%) (χ2 =5.76, P=0.020).The d/D values were 0.51±0.06,0.54±0.08,0.57±0.09,0.61±0.09,0.59±0.07 respectively at abovementioned 5 flexion positions, which were different among groups( F = 3.450 ,P = 0.013 ).The value was greater at 35° than that at 20° and 25°( P <0.05 ), and it was also greater at 40° than that at 20° ( P < 0.05 ).Conclusion Cervical flexion angle has an effect on anterior shifting of the posterior wall of the cervical dural canal and widening of epidural space.
10.Expression of hsa-miR-20a in human glioma tissues and its effect on the proliferation of human glioma cells in vitro.
Xingjun YAO ; Wei LI ; Yuehua WANG ; Zhengbin DING ; Wenzhong HOU ; Minmin ZENG ; Guangce DENG ; Jinzhu ZHANG ; Hongfa YANG
Journal of Southern Medical University 2012;32(2):198-201
OBJECTIVETo investigate miR-20a expression in human glioma and normal brain tissues and its effect on the proliferation of glioma cells in vitro.
METHODSThe expression of miR-20a was detected in human normal brain tissues and glioma tissues by real-time RT-PCR. miR-20a mimics were synthesized and transfected into U251 cells via liposome, and the cell proliferation were detected using MTT assay and flow cytometry.
RESULTSThe glioma tissues showed significantly up-regulated expression of miR-20a compared with normal brain tissues (P=0.035). The expression level of miR-20a was higher in high-grade than in low-grade gliomas. miR-20a mimics significantly enhanced the proliferation of U251 cells and the percentage of S-phase cells.
CONCLUSIONmiR-20a shows potent effect in promoting the growth of glioma cells, suggesting its important role in the pathogenesis of human glioma.
Adult ; Brain Neoplasms ; genetics ; metabolism ; pathology ; Cell Line, Tumor ; Cell Proliferation ; Female ; Glioma ; genetics ; metabolism ; pathology ; Humans ; Male ; MicroRNAs ; genetics ; metabolism ; Middle Aged ; Real-Time Polymerase Chain Reaction ; Young Adult