Objective:To investigate the preventive and therapeutic effects of intraperitoneal injectoin of IFN? on bronchial asthma in mice and the relevant mechanism. Methods: Thirty-six BALB/c mice were randomly equalized into 3 groups:group A (normal control group),group B (asthmatic model group) and group C (IFN? treated group). The asthmatic model was established in group B and C by immunization with ovalbumin (OVA) absorbed to aluminum hydroxide. Mice of group B and C received 0.25 ml PBS and 5 ?g IFN? intraperitoneally on days 23 to 30 once daily prior to ovalbumin challenge,respectively. Bronchoalveolar lavage fluid (BALF) was collected on day 31 for determining the cellular composition and the concentrations of IL-4 and IL-5. Meanwhile,IgE in serum was determined. The pathological changes and the expression of GATA-3 were investigated in the lungs of mice. Results: (1) BALF eosinophils was significantly decreased in group C compared with those in group B ( vs ,P

Objective To investigate the role of IFN-? in prevention and treatment of bronchial asthma and the mechanism of its effect. Methods BALB/C mice were randomly divided into three groups: group A (control group, n=12); group B (asthma model group, n=12); group C (IFN-? intraperitoneal treatment group, n=12). The asthma model was reproduced in group B and C with ovalbumin (OVA) adsorbed to aluminum hydroxide. PBS (0.25ml) and IFN-? 5?g was respectively injected intraperitoneally in group B and C on days 23 to 30 once daily prior to ovalbumin challenge. Bronchoalveolar lavage fluid (BALF) was collected on day 31 and its cellular composition was analyzed. The levels of IL-4, IL-5 and the IgE in serum were determined. The pathological changes in the lung and the expression of GATA-3 were observed. Results A notable decrease of eosinophils (0.3?0.2) in BALF was found in group C comparing with the group B (21.1?6.7) (P

In order to deepen the reform of public hospitals,Beijing Tongren Hospital has carried out the pilot effort of famous experts team service model in out-patient,established a new mode of interior hierarchical medical services in the hospital.Through the adjustment of outpatient service process and the change of service model,issues of difficult medical treatment and ticket scalpers were solved.The reform has achieved the optimal utilization of outpatient resources inside the hospital,has been the beneficial exploration and practice of hospital internal hierarchical diagnosis and treatment,truly reflected the hospital service concept of taking the patient as the center,and has laid a solid foundation for the future widely hierarchical diagnosis and treatment mode.

Objective To investigate the apoptosis induction effect of recombinant caspase-3 expression on osteosarcoma cell line SOSP-9901. Methods Recombinant caspase-3 gene was subcloned into the GFP reporter vector pEGFP-C1 to generate the expression vector pEGFP-caspase-3 by DNA recombinant technique. pEGFP-caspase-3 was transfected into human osteosarcoma cell line SOSP-9901 by Lipofectamine 2000. The expression of recombinant caspase-3 was determined by RT-PCR. The morphological changes of transfected cells were observed under flurescent and electronic microscope. The cell survival rate of transfected cells was assayed by MTT method. Results Recombinant caspase-3 gene could be stably expressed in the transfected SSOP-9901 cells. Recombinant caspase-3 could obviously induce SOSP-9901 apoptosis, and inhibit SSOP-9901 cell proliferation in vitro. Conclusion Recombinant caspase-3 could inhibit the growth of the osteosarcoma cell line SOSP-9901 and induce it into apoptosis.

Objective: To investigate the characteristics of lower respiratory tract infection caused by Chryseobacterium and its antimicrobial susceptibility in vitro . Methods: The clinical data of 52 cases of lower respiratory tract infection caused by Chryseobacterium were analyzed, and the antimicrobial susceptibility of commonly used agents was determined with the method of agar dilution. Also the 50% lethal dosage (LD 50 ) (as the marker of virulence) of 20 randomly selected Chryseobacterium strains for mice were determined. Results: (1) Thirty six was over 60 years old;all of 52 cases had underlying diseases, mainly were chronic obstruction pulmonary disease and malignant tumors. Seventeen cases had the history of incubation or tracheotomy for mechanical ventilation, and 35 had history of broad antibiotics treatment. The mean hospitalization time before infection were 35.6 d, and 38.5% of the cases had mixed infection with other bacteria. No specific clinical manifestations and chest X ray appearance revealed. (2) The in vitro activity of 25 agents showed that these strains were highly resistant. (3) The range of the LD 50 of tested strains was 4.11?10 6 5.68?10 8/mouse, suggesting low virulence of this kind of bacteria. Conclusion: The lower respiratory tract infection caused by Chryseobacterium has no unique features; the incidence of the infection increases in immunosuppressed old patients with various underlying diseases, although the virulence is relatively low. Because the clinical isolates are highly resistant, the antibiotics should be selected according to the results of bacterial sensitivity test.

Objective:To explore the effect of nontypeable Haemophilus influenzaen(NTHi)strain ATCC49247on pri-mary rabbit tracheal epithelial(TE)cells.Methods:TE cells were isolated with low temperature protease digestion and cul-tured on collagen gel-coated membranes at an air-liquid interface in serum-free medium.Under these conditions,TE cells were proliferated and differentiated into a pseudostratified mucociliary epithelium,which were infected by NTHi.Morphologic changes of the cells were examined by scanning electron microscopy(SEM)and transmission electron microscopy(TEM)after 24h.Results:SEM showed that bacteria adhered to non-ciliated cells;death or apoptosis occurred in90%of TE cells and cil-iaries were broken.TEM showed NTHi adhered to the cell surface on which there were many microvillus.Lamellipodia and microvilli surrounded bacteria within vacuoles of airway cells.Conclusion:NTHi can attach to non-ciliated cells,the latter de-vours the bacteria by lamellipodia and microvilli.NTHi is toxic to TE cells,resulting in the death or apoptosis of TE cells.

The relationship between subclinical hypothyroidism (SCH) and the components of metabolic syndrome (MS) was explored.A total of 2 252 subjects aged 20 to 79 years from medical examination were identified.The prevalence of SCH was 4.88％.The rate of 7.30％ in female was markedly higher than 2.17％ in male(P＜0.01).The highest SCH prevalence in adults was in the ≥70 years old group.The prevalence of SCH in female was increased with age(P＜0.01).Compared with the euthyroid group,body mass index,triglyceride,and low density lipoprotein-cholesterol were significantly elevated (P＜0.05),while high density lipoprotein-cholesterol was significantly decreased in SCH group(P＜0.05).The incidences of hypertension,dyslipidosis,and MS in SCH group were significantly higher than those in euthyroid group(P＜0.05 or P＜0.01) ; Serum TSH in the MS group was higher than that in the Non-MS group[(2.43 ± 1.55 vs 2.21 ± 1.48) mU/L,P＜0.01].The incidence of SCH in MS group was significantly higher than that in the Non-MS group (7.69％ vs 4.25％,P＜0.05).The prevalence of SCH in women was increased with age.Multiple factors of dysbolism were clustered in subjects with SCH.There is a correlation between SCH and MS.

Objective: To investigate the effect of CpG motif oligodeoxynucleotides (CpG ODN) on the antigen induced allergic airway reaction in mice. Methods: The asthma model was set up in the C57BL/6 mice with OVA, the CpG ODN in the dose of 30 ?g was co administered intraperitoneally with the antigen in sensitization stage to study its effect on the airway allergenic reactions. Results: (1)Compared with the control, coadministration of CpG ODN in sensitization phase significantly inhibited airway eosinophilia after antigen challenge( P

OBJECTIVE:To provide suggestions for the government decision of payment price of medicare drugs. METHODS:Investigation was conducted for the beginning of payment price policy of medicare drugs,effect of the implementation of policy in other countries and the encountered problems. The payment pricing mechanism was explored based on the combination of new medi-cal reform with theoretical analysis and empirical research. RESULTS:The reform of payment pricing mechanism of medicare drugs will have effect on current drug price,especially for R&D oriented pharmaceutical industries. The policy can decrease drug expendi-ture in medicare expenditure,and it is not sure whether it can decrease total medicare expenditure. CONCLUSIONS:It is suggest-ed to notice the related measures and government should keep balance between drug availability and encouraging R&D innovation.

Objective　To identify the regression of left ventricle after aortic valve replacement for aortic regurgitation complicated by extremely dilated left ventricle. Methods　34 cases were reviewed to evaluate the regression of left ventricle by UCG. Results　All patients survived after aortic valve replacement. The left ventricular end diastolic diameter (LVEDD) decreased dramatically 1－2 weeks and further decreased 6－12 months after operation, i.e. 87.2±6.6 mm (preoperation), 70.2±13.5 mm (1－2 weeks post-operation), and 62.0±12.52 mm (6－12 months post-operation), respectively, P<0.01. If the patients were grouped according to left ventricular ejection fraction (LVEF) before operation: group A(LVEF<50%) and group B (LVEF>50%), reduction of LVEDD and increase of LVEF in group B were more significant than in group A. Conclusion　LVEF is the key factor for the regression of left ventricle and can be used to predict the prognosis.