Sorghum (Sorghum bicolor L.) was one of the most important crops in the world next to wheat, rice, maize, soybean and barley. Using the callus derived from immature inflorescence as the recipients, we efficiently transformed sorghum varieties 115, ICS21B and 5-27 with the insecticidal Bacillus thuringiensis (Bt) cry1Ab gene carried in the T-DNA of binary vectors which contained hygromycin resistance gene and gus gene via Agrobacterium tumefaciens. After gradient selection with hygromycin, a total of 21 independent transgenic plant lines, 52 transgenic plants were regenerated, and the average stably transformation efficiency was 1.9%. The integration and transcription of cry1Ab gene in transgenic sorghum was confirmed by PCR analysis, Southern blotting and RT-PCR analysis. The Bt proteins were expressed in most transgenic plants with different level from plant to plant by Western blotting and ELISA assay. According to insect bioassay in laboratory, the transgenic plants with a relatively high level of Bt gene expression displayed insect-resistance to pink rice borer (Sesamina inferens).
Agrobacterium tumefaciens ; genetics ; metabolism ; Bacterial Proteins ; genetics ; metabolism ; Endotoxins ; genetics ; metabolism ; Hemolysin Proteins ; genetics ; metabolism ; Pest Control, Biological ; Plants, Genetically Modified ; genetics ; Sorghum ; genetics ; Transformation, Genetic

Bakanae is an emerging rice disease caused by the seed-and soil-borne pathogen Fusarium fujikuroi.It is becoming a more serious threat to sustainable rice production throughout rice-growing regions.Bakanae disease infection is responsible for high yield losses ranging from 3%to 95%,and disease incidence varies based on the region and cultivars.Hence,understanding the nature of the pathogen,its pathogenicity,disease epidemiology,symptoms,host-pathogen interaction,and the role of secondary metabolites in the disease cycle will be helpful in the development of effective and sustainable management strategies.However,very few comprehensive studies have described the details of rice bakanae disease.Thus,in this review we summarize and discuss in detail the information available from 1898 to 2023 on various critical facets of bakanae disease,and provide perspectives on future research.